Introduction: One of the most prevalent malignant tumors is osteosarcoma. The use of herbal medicines that can suppress or kill cancer cells through their antioxidant properties is growing in popularity nowadays as a result of the numerous negative effects of cancer medications. Different parts of M.emarginata have been mentioned to be therapeutically used as diuretic and neuralgia. The main aim of this study is to find out an antiproliferative activity of M.emarginataleaf extract on SAOS 2 cell lineMaterials and Methods: The Merremia emarginata(burm.F.) leaf were soxhlet extracted. Cultured Saos Cells were stimulated with various concentrations of M.emarginata(burm.F.) leaf extracts. To dissolve the formazan formed from MTT, the cells were resuspended in dimethyl sulfoxide (DMSO). The inhibitory rate of cell growth was calculated. mRNA expression levels were examined using real-time PCR.Results: As the concentration of leaf extract increases from 50μg/ml to 200μg/ml the cell viability is significantly decreased. Bcl2 and Bcl-xl expression were significantly negatively correlated with higher concentration of Saos -2 cells treated with M.emarginata.Conclusion: The study revealed that M.emarginatahas a significant role in controlling bone cancer cell proliferation by upregulating caspase 3 mRNA and down regulating the gene expression of Bcl 2 and Bcl xl.
Introduction: Bixaorellanais a species of tree in the family Bixaceae. The common usage of Bixaorellana include antipyretic, aphrodisiac, anti diarrhoeal, antidiabetic and insect repellant. HepG2 is an immortal cell line used as a model system for studies of liver metabolism and toxicity of xenobiotics, detection of environmental and dietary cytotoxic and genotoxic agents, understanding hepatocarcinogenesis and for drug targeting studies. The main aim of this study is to prepare Bixaorellana bark extract and to determine the cytotoxic effects of the extract on HepG2 cell line.Materials and Methods: The Bixaorellana bark extract was made using Soxhlet extraction and concentrated using Rotary evaporator. The cell viability of HepG2 cells was measured using MTT Assay. Then the gene expression analysis of the HepG2 cells was done by RNA isolation, complementary DNA synthesis followed by Real time PCR to analyze the anticancer activity.Results: As the concentration level increases, the cell viability dose dependently decreases. By modulating the expression of apoptotic signaling molecules, the drug was able to inhibit the proliferation of the cancer cells.Conclusion: This study demonstrated that bark extracts of Bixaorellanawere cytotoxic against BCl 2 mRNA and BCl-xL mRNA induced apoptosis on the human cells. Active components were probably responsible for anticancer activity. MTT assay showed that as the concentration of treated Bixaorellana bark extract increases, the cancer cell viability decreases.
Background: B.serrata is commonly known as Indian olibanum, it is mainly used in the production of Indian frankincense. B.serrata is used for many other purposes including asthma diabetes. The medicinal plant Boswelliaserrata has great potential for treating cancer. Since many cancer therapies have their origins in natural ingredients, this study aims to explore the anti-tumorigenic effect of B.serrata using KB cell line.Materials and methods: Seed of B.serrata was collected and extraction was done. Then the extract was added to the culture and the oral cancer cells were obtained from Pune. The cultured oral cells were incubated in a CO2 incubator. Treated oral cancer cell sample was done MTT assay and gene expression analysis.Result: The collected data from MTT assay and gene expression analysis tabulated using SPSS software. MTT assay results showed gradual decrease in cell viability with increase in concentration of Boswelliaserrata and the gene expression showed up regulation of caspase 3 and 9 expression.Conclusion: From the study, Boswelliaserrata has a significant effect on anti-tumorigenic activity; this might be due to the active bio components present in Boswelliaserrata extract.
Introduction: Cancer, one of the most researched diseases worldwide, researches is being done to develop new technology and more effective treatments. Still, there are many gaps in the cancer treatments that are now on the market that have a negative impact on patients' health in the form of side effects. A better approach to treat this awful disease is to use herbal drugs made from medicinal plants. Since ancient times, different illnesses have been treated with the well-known Ayurvedic medicinal plant Withania somnifera L. Dunal (Solanaceae).The aim of this study is to analyze the anti-cancer activity of With aniasomnifera against human liver cancer cells.Materials and methods: Withania somnifera root powder was extracted as viscous mass by undergoing soxhlet extraction and then rotary evaporation. After culturing of HepG2 cells and treatment with the extract, it was subjected to MTT assay and Real time PCR to identify the anti-cancer activity.Results: MTT assay showed that as the concentration of treated W.somnifera ethanolic extract increases, the cancer cell viability decreases. As the concentration of the extract increased, the cell viability decreased. The W.sominifera performed by altering the expression of the apoptotic signaling molecules Bcl 2 and BclXl to suppress the growth of cancer cells.Conclusion: The ethanolic extract of Withaniasomnifera down regulating the expression of Bcl 2 and Bcl XL mRNA on human liver cancer cells and induced apoptosis of the cells. The most effective anticancer substances are found in W.somnifera, which also induces apoptosis without causing any negative side effects.
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