Effect of obstructive jaundice on wound healing

Arnaud, Jean‐Pierre; Humbert, W.; Eloy, M.R.; Adloff, M

doi:10.1016/0002-9610(81)90059-3

Cited by 30 publications

(16 citation statements)

References 7 publications

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“…There is evidence in the literature that jaundice interferes negatively with the healing process [6][7][8] . However, this was not evident in the present study when comparing groups L vs. M and LK vs. M, whether on histological analysis or tensile strength analysis of the sutured bowel segment.…”

Section: Discussionmentioning

confidence: 99%

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Effect of vitamin K1 supplementation on left colon healing in rats with extrahepatic biliary obstruction

Amaral¹,

Alecrim²,

Duarte³

et al. 2014

Acta Cir. Bras.

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Intellectual and scientific content of the study, protocol design, provision of guidelines for surgical intervention, supervision of all phases of the study, manuscript writing, critical revision. ABSTRACT PURPOSE:To evaluate the effects of vitamin K1 on wound healing in the left colon of rats with experimental biliary obstruction. METHODS:Sixteen male rats, divided into four groups of four animals each (L, M, LK, and MK), underwent colostomy followed by bowel suture in the left colon. Seven days before, animals in the L and LK groups had undergone common bile duct ligation. The animals in groups MK and LK received vitamin K1 supplementation. On day 7 after bowel suture, repeat laparotomy was performed for evaluation of colonic healing by burst pressure measurement and collection of samples for histopathological analysis. Changes in body weight were evaluated in the four groups. RESULTS:Weight loss was lower in animals supplemented with vitamin K. No significant differences were observed in burst pressure among the four groups (p>0.05). Histological analysis showed more hemorrhage and congestion in the biliary obstruction groups.Supplemented animals exhibited increased collagen formation and less edema and abscess formation. CONCLUSION:Vitamin K supplementation attenuated weight loss and improved colonic wound healing in rats.

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Section: Discussionmentioning

confidence: 99%

“…There is evidence that obstructive jaundice contributes to poor outcomes of anastomotic bowel healing [6][7][8] . In 1997, Brazilian investigators reviewed the literature on the subject and, after presenting data from their own experimental procedure, reported possible mechanisms responsible for failed healing in jaundiced conditions.…”

Section: Introductionmentioning

confidence: 99%

Effect of vitamin K1 supplementation on left colon healing in rats with extrahepatic biliary obstruction

Amaral¹,

Alecrim²,

Duarte³

et al. 2014

Acta Cir. Bras.

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“…Ligation of the common bile duct in rodents is associated with increased fibroblast proliferation and fibrosis (52). This condition is also associated with a delayed or impaired wound healing of the skin combined with a weaker scar (3,13). Bile and bile acids in particular have been suggested to be central to the pathologies associated to disorders of hepatic, coagulation, renal, skin, or immune function coincident with deep jaundice, which can be reduced via biliary drainage (36).…”

Section: Discussionmentioning

confidence: 99%

Biphasic regulation by bile acids of dermal fibroblast proliferation through regulation of cAMP production and COX-2 expression level

Jian

Ceryak²,

Aratsu³

et al. 2006

American Journal of Physiology-Cell Physiology

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-We have previously reported that the bile acids chenodeoxycholate (CDCA) and ursodeoxycholate (UDCA) decreased PGE1-induced cAMP production in a time-and dose-dependent manner not only in hepatocytes but also in nonhepatic cells, including dermal fibroblasts. In the present study, we investigated the physiological relevance of this cAMP modulatory action of bile acids. PGE1 induced cAMP production in a time-and dosedependent manner. Moreover, PGE1 (1 M), forskolin (1-10 M), and the membrane-permeable cAMP analog CPT-cAMP (0.1-10 M) decreased dermal fibroblast proliferation in a dose-dependent manner with a maximum inhibition of ϳ80%. CDCA alone had no significant effect on cell proliferation at a concentration up to 25 M. However, CDCA significantly reduced PGE1-induced cAMP production by 80 -90% with an EC50 of ϳ20 M. Furthermore, at concentrations Յ25 M, CDCA significantly attenuated the PGE-1-induced decreased cell proliferation. However, at concentrations of 50 M and above, while still able to almost completely inhibit PGE-1-induced cAMP production, CDCA, at least in part through an increased cyclooxygenase-2 (COX-2) expression level and PGE2 synthesis, produced a direct and significant decrease in cell proliferation. Indeed, the CDCA effect was partially blocked by ϳ50 -70% by both indomethacin and dexamethasone. In addition, overexpression of COX-2 cDNA wild type resulted in an increased efficacy of CDCA to block cell proliferation. The effects of CDCA on both cAMP production and cell proliferation were similar to those of UDCA and under the same conditions cholate had no effect. Results of the present study underline pathophysiological consequences of cholestatic hepatobiliary disorders, in which cells outside of the enterohepatic circulation can be exposed to elevated bile acid concentrations. Under these conditions, low bile acid concentrations can attenuate the negative hormonal control on cell proliferation, resulting in the stimulation of cell growth, while at high concentrations these bile acids provide for a profound and prolonged inhibition of cell proliferation. chenodeoxycholic acid; cyclic adenosine monophosphate cAMP, which can be induced by the prostaglandins (PG) PGE1 and PGE2, is known to have both proliferative and antiproliferative effects, according to cell type. For example, cAMP stimulates the proliferation of cells, such as hepatocytes and 3T3 cells, while inhibiting the proliferation of normal human skin fibroblasts, gastric cells, and hepatic stellate cells (20,30,38,55). The PGE2 is also a potent inhibitor of fibroblast migration, proliferation and collagen synthesis (23,24,54). PGs are derived from arachidonic acid through the cyclooxygenase (COX)

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“…Hyperbilirubinemia could have negative effects on healing as it impairs the proliferation of fibroblasts and immune cells 3 . Furthermore, cholestasis is associated with changes in liver function, which can affect the processes involved in healing 4 .…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have suggested that obstructive jaundice is associated with increased incidence of surgical complications, such as anastomotic dehiscence, incisional hernia, and surgical site infection [1][2][3] . Hyperbilirubinemia could have negative effects on healing as it impairs the proliferation of fibroblasts and immune cells 3 .…”

Section: Introductionmentioning

confidence: 99%

Effect of glutamine supplementation on left colon healing in rats with extrahepatic biliary obstruction

Alecrim¹,

Duarte²,

Amaral

et al. 2015

Acta Cir. Bras.

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Intellectual and scientific content of the study, designed the protocol, provided guidelines for the surgical interventions, supervised all phases of the study, manuscript writing, critical revision. ABSTRACT PURPOSE:To evaluate the influence of glutamine and obstructive jaundice on left colon healing in rats. METHODS:Sixteen male rats were allocated across four groups: LG -Common bile duct ligation followed by colotomy and bowel suture on postoperative day 7. Supplementation with glutamine 2% from day 4 after duct ligation until euthanasia. L -Common bile duct ligation followed by colotomy and bowel suture on postoperative day 7. No glutamine supplementation. M -Common bile duct manipulation followed by colotomy and bowel suture on postoperative day 7. No glutamine supplementation. MG -Common bile duct manipulation followed by colotomy and bowel suture on postoperative day 7. Supplementation with glutamine 2% from day 4 after duct manipulation until euthanasia. On the day of euthanasia, bursting pressure of the sutured bowel segment was measured and samples were collected for histopathological analysis. RESULTS:There were no differences in bursting pressure among groups : LG vs. M (110 ± 28 vs. 173 ± 12; p = 0.08). Groups L and MG were not different from group M (156 ± 12 and 118 ± 22. Glutamine supplementation was associated with less edema, polymorphonuclear lymphocyte infiltration, bacterial colonies, and abscess formation, as well as with increased collagen formation. CONCLUSION:Obstructive jaundice had no negative effect and glutamine supplementation had no positive effect on colonic scar strength in rats.

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Effect of obstructive jaundice on wound healing

Cited by 30 publications

References 7 publications

Effect of vitamin K1 supplementation on left colon healing in rats with extrahepatic biliary obstruction

Effect of vitamin K1 supplementation on left colon healing in rats with extrahepatic biliary obstruction

Biphasic regulation by bile acids of dermal fibroblast proliferation through regulation of cAMP production and COX-2 expression level

Effect of glutamine supplementation on left colon healing in rats with extrahepatic biliary obstruction

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