Effect of Nutrients on Environmental Sex Determination and Size of Gametophytes in Culcita macrocarpa

Ghosh, Lipika; Jiménez, Ares; Quintanilla, Luís G.

doi:10.3329/jles.v7i0.20130

Cited by 4 publications

(2 citation statements)

References 30 publications

(18 reference statements)

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“…Hermaphroditic gametophytes of C. barometz were larger than male gametophytes, which were larger than asexual gametophytes. These results are similar to the gametophyte observations on Culcita macrocarpa reported by Ghosh et al (2012). , asexual); B. Ribbon-like shape (male); C-D. Spatulate shape (male); E-F. Heart shape (bisexual); G-H. Long heart shape (male), white arrow showing antheridia at abaxial surface.…”

Section: High Population Density Affects Gametophyte Growth and Morphsupporting

confidence: 78%

Short Communication: Selected medicinal plants in East and North Kalimantan (Indonesia) against Propionibacterium acnes

et al. 2017

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Abstract. Praptosuwiryo TNg, Isnaini Y. 2017. Morphological variations and sex expression in gametophytes of . Characteristics of gametophytes in ferns have been shown to be phylogenetically significant. Studies of fern gametophytes have become essential to fill the gaps in our knowledge of fern morphology, ecology, reproduction, evolution and distribution. The purposes of this study were: (i) to observe morphological variations and sex expression in gametophytes of Cibotium barometz (L.) J. Sm., and (ii) to understand how gametophyte densities affect sex expression in the species. Spores of five collection numbers of C. barometz from Sumatra, Indonesia, were sown on half-strength Murashige & Skoog (½MS) basal medium. Eleven weeks after germination of spores, prothalli were subcultured on ½MS medium along with sugar (30 g/L) and Naphthalene Acetic Acid (NAA, 0.5 mg/L). After eight months subculturing of prothalli (prothallus density: 100-150 individuals per cm 2 ), 100 gametophytes were observed for each collection number to determine their shapes and sex expression. Between 9-12 months after subculturing, gametophytes growing at different population densities (between 100-500 individuals per cm 2 ) were sampled. One hundred prothalli were selected among the ten replicates for each collection number. The percentage of each identified gametophyte shape and their genders were recorded. Eight morphological types of adult gametophyte were recorded: (i) Branching filament (asexual), (ii) ribbon-like shape (male), (iii) spatulate shape (asexual, male, female), (iv) heart shape (male, female, bisexual), (v) gemmiferous heart shape (asexual, female, bisexual), (vi) long heart shape (male, bisexual); (vii) gemmiferous long heart shape (asexual, male, bisexual), and (viii) gemmiferous irregular shape (asexual, male, female, bisexual). We conclude that gametophyte morphology is simply "too plastic" to be used in suporting species delimitation in ferns if the prothalli is to be cultured in a high population density. There is a correlation between gametophyte size, shape and sex expression that is related to the population density. The presence of unisexual and bisexual gametophytes indicates that both intergametophytic and intragametophytic selfing occur in C. barometz.

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Section: High Population Density Affects Gametophyte Growth and Morphsupporting

confidence: 78%

Short Communication: Selected medicinal plants in East and North Kalimantan (Indonesia) against Propionibacterium acnes

et al. 2017

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“…The filamentous gametophyte is a very simple morphological structure, consisting of a few cells dividing in the same orientation before switching to two-dimensional growth. In addition, its vulnerable morphology is strongly influenced by the environment [75].…”

Section: Response To Stimulus and Defense Processes Are Enriched In O...mentioning

confidence: 99%

Differential gene expression profiling of one- and two-dimensional apogamous gametophytes of the fern Dryopteris affinis ssp. affinis

Wyder

Rivera

Valdés

et al. 2020

Plant Physiology and Biochemistry

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Effects of variation in carbon, nitrogen, and phosphorus molarity and stoichiometry on sex determination in the fernCeratopteris richardii

Goodnoe

Hill

Aho

2016

Botany

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Carbon (C), nitrogen (N), and phosphorous (P) are needed by all organisms for basic biological processes. When an individual macronutrient is not accessible, nutrient limitation occurs. The stochiometric balance between multiple nutrients and individual concentrations are both vital for normal growth and development. Labile sex expression in plants is a phenotypic trait predicted to be sensitive to local nutrient conditions because males and females differ in their nutritional demands. We applied concepts from ecological stoichiometry to assess the effects of variation in individual nutrient concentration and multiple macronutrient stoichiometry on sexual development in the fern Ceratopteris richardii Brongn. Manipulation of N, P, and organic and inorganic C was expected to yield variation in the ratio of males to females, consistent with environmental sex-determination theory. Our results suggest nutrient stoichiometry, not strictly concentration, influences sex determination at ambient CO2. However, an early response to population density preempted nutrient effects in elevated CO2 environments with exogenous glucose, in which C. richardii gametophytes presumably grow naturally. Although sex determination is not nutrient-dependent in the latter environment, C:N in the dry mass of meristic gametophytes is influenced by the external nutrient context, suggesting sex determination takes place before abiotic environmental factors subsequently influence plant nutrient uptake.

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Effect of Nutrients on Environmental Sex Determination and Size of Gametophytes in Culcita macrocarpa

Cited by 4 publications

References 30 publications

Short Communication: Selected medicinal plants in East and North Kalimantan (Indonesia) against Propionibacterium acnes

Short Communication: Selected medicinal plants in East and North Kalimantan (Indonesia) against Propionibacterium acnes

Differential gene expression profiling of one- and two-dimensional apogamous gametophytes of the fern Dryopteris affinis ssp. affinis

Effects of variation in carbon, nitrogen, and phosphorus molarity and stoichiometry on sex determination in the fernCeratopteris richardii

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