Effect of Novel Marine Nutraceuticals on IL-1<i>α</i>-Mediated TNF-<i>α</i>Release from UVB-Irradiated Human Melanocyte-Derived Cells

Muthusamy, Visalini; Hodges, Lynn D.; Macrides, Theodore A.; Boyle, Glen M.; Piva, Terrence J.

doi:10.1155/2011/728645

Cited by 13 publications

(16 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Apart from changes to cell signaling activity, UV radiation can alter cytokine levels in melanocyte-derived cells [17]. Of interest is tumor necrosis factor-α (TNFα), a proinflammatory cytokine, which may be involved in anti- or pro-tumor activities in melanoma development [11,18].…”

Section: Introductionmentioning

confidence: 99%

UVB-Stimulated TNFα Release from Human Melanocyte and Melanoma Cells Is Mediated by p38 MAPK

Muthusamy

Piva

2013

IJMS

Self Cite

View full text Add to dashboard Cite

Ultraviolet (UV) radiation activates cell signaling pathways in melanocytes. As a result of altered signaling pathways and UV-induced cellular damage, melanocytes can undergo oncogenesis and develop into melanomas. In this study, we investigated the effect of UV-radiation on p38 MAPK (mitogen-activated protein kinase), JNK and NFκB pathways to determine which plays a major role in stimulating TNFα secretion in human HEM (melanocytes) and MM96L (melanoma) cells. MM96L cells exhibited 3.5-fold higher p38 activity than HEM cells at 5 min following UVA + B radiation and 1.6-fold higher JNK activity at 15–30 min following UVB+A radiation, while NFκB was minimally activated in both cells. Irradiated HEM cells had the greatest fold of TNFα secretion (UVB: 109-fold, UVA + B: 103-fold & UVB+A: 130-fold) when co-exposed to IL1α. The p38 inhibitor, SB202190, inhibited TNFα release by 93% from UVB-irradiated HEM cells. In the UVB-irradiated MM96L cells, both SB202190 and sulfasalazine (NFκB inhibitor) inhibited TNFα release by 52%. Although, anisomycin was a p38 MAPK activator, it inhibited TNFα release in UV-irradiated cells. This suggests that UV-mediated TNFα release may occur via different p38 pathway intermediates compared to those stimulated by anisomycin. As such, further studies into the functional role p38 MAPK plays in regulating TNFα release in UV-irradiated melanocyte-derived cells are warranted.

show abstract

Section: Introductionmentioning

confidence: 99%

UVB-Stimulated TNFα Release from Human Melanocyte and Melanoma Cells Is Mediated by p38 MAPK

Muthusamy

Piva

2013

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, the steroids also inhibit STAT3 proteins (signal transducers and activators of transcription), which are important in cancer cell survival and proliferation (Aggarwal and Shishodia 2006). In addition, the mussel oil extract did not affect the release of TNF-a, an inflammatory and cancer mediator, by suppressing p38 MAPK pathway in human melanocyte cell (Muthusamy et al 2011). On the contrary, cholesterol treatment increased endothelial nitric oxide synthase (eNOS) expression (synthesis of nitric oxide) (Peterson et al, 1999), which was similar to the results of this study that cholest-7-en-3-ol did not show both cell toxicity and anti-inflammatory activity determined through the NO inhibition and cytokine assays on COX-2 and TNF-a (data not shown).…”

Section: Purification Of Anti-inflammatory and Anticancer Compoundsmentioning

confidence: 89%

“…New Zealand green-lipped mussel powder (lyprinol) had significant anti-inflammatory activity when given to animals and humans (Halpern 2000), where much of this activity was associated with lipid (omega-3 PUTAs) and carotenoids. 5b-Scymnol from mussel oil Perna canaliculus prevented the UV damage to melanocytes and regulated the proinflammatory cytokine and TNF-a (Muthusamy et al 2011). However, there is no detailed information on the anti-inflammatory compounds of horse mussel.…”

Section: Anti-inflammatory Activitymentioning

confidence: 99%

“…The molluscs have become one of the most important sources of marine natural products in the last decades, and a significant number of compounds with unusual structures and bioactivities have been isolated from various molluscs. There are a few reports on the biological activities of shellfishes origin steroid compounds including cholesterols: a mediator of inflammation (Antonio et al 2011), proinflammatory cytokine and TNF-a (Muthusamy et al 2011), anti-inflammatory activity of New Zealand green-lipped mussel (Halpern 2000), antibacterial activity of horse mussel Modiolus modiolus (Haug et al 2004), and antioxidant activities of black mussel Mytilus galloprovincialis (Gorinstein et al 2003). In particular, molluscs are considered to be one of the most promising sources due to their variety of species and applications.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nitric oxide synthesis inhibition and cytotoxicity of Korean horse mussel Modiolus modiolus extracts on cancer cells in culture

Wikarta

Kim

2015

Cytotechnology

View full text Add to dashboard Cite

The Korean horse mussel extract was purified and fractionated by a bioassay-guided purification step. The final fraction contained seven steroid and one polycyclic aromatic compounds, in which cholest-7-en-3-ol, (3b,5a)-(58.7 %) was a main component followed by ergosta-7,22dien-3-ol (3b,5a,22E) (13.0 %). This extract exhibited strong anti-inflammatory activity determined solely through the nitric oxide inhibition assay in a dose-dependant manner with the IC 50 value of 9.6 lg/mL and no cytotoxic effect on the macrophages. Moreover, it also exhibited strong cytotoxicity with the IC 50 values of 21.4, 36.4, and 37.1 lg/mL against AGS, DLD-1, and HeLa cells, respectively. These results indicated that the horse mussel extract might be a functional ingredient in the prevention of inflammation and human cancers.

show abstract

“…This is particularly the case in the public maternity care setting, in which around two thirds of Australian women receive care [1,2]. The impetus to introduce these new models is likely to be multifactorial, and ‘driven’ by different stakeholders, i.e.…”

Section: Introductionmentioning

confidence: 99%

Exploring implementation and sustainability of models of care: can theory help?

et al. 2011

View full text Add to dashboard Cite

ObjectiveResearch on new models of care in health service provision is complex, as is the introduction and embedding of such models, and positive research findings are only one factor in whether a new model of care will be implemented. In order to understand why this is the case, research design must not only take account of proposed changes in the clinical encounter, but the organisational context that must sustain and normalise any changed practices. We use two case studies where new models of maternity care were implemented and evaluated via randomised controlled trials (RCTs) to discuss how (or whether) the use of theory might inform implementation and sustainability strategies. The Normalisation Process Model is proposed as a suitable theoretical framework, and a comparison made using the two case studies – one where a theoretical framework was used, the other where it was not.Context and approachIn the maternity sector there is considerable debate about which model of care provides the best outcomes for women, while being sustainable in the organisational setting. We explore why a model of maternity care – team midwifery (where women have a small group of midwives providing their care) – that was implemented and tested in an RCT was not continued after the RCT’s conclusion, despite showing the same or better outcomes for women in the intervention group compared with women allocated to usual care. We then discuss the conceptualisation and rationale leading to the use of the ‘Normalisation Process Model' as an aid to exploring aspects of implementation of a caseload midwifery model (where women are allocated a primary midwife for their care) that has recently been evaluated by RCT.DiscussionWe demonstrate how the Normalisation Process Model was applied in planning of the evaluation phases of the RCT as a means of exploring the implementation of the caseload model of care. We argue that a theoretical understanding of issues related to implementation and sustainability can make a valuable contribution when researching complex interventions in complex settings such as hospitals.Conclusion and implicationsApplication of a theoretical model in the research of a complex intervention enables a greater understanding of the organisational context into which new models of care are introduced and identification of factors that promote or challenge implementation of these models of care.

show abstract

Effect of Novel Marine Nutraceuticals on IL-1α-Mediated TNF-αRelease from UVB-Irradiated Human Melanocyte-Derived Cells

Cited by 13 publications

References 49 publications

UVB-Stimulated TNFα Release from Human Melanocyte and Melanoma Cells Is Mediated by p38 MAPK

UVB-Stimulated TNFα Release from Human Melanocyte and Melanoma Cells Is Mediated by p38 MAPK

Nitric oxide synthesis inhibition and cytotoxicity of Korean horse mussel Modiolus modiolus extracts on cancer cells in culture

Exploring implementation and sustainability of models of care: can theory help?

Contact Info

Product

Resources

About