Effect of Novel Bioactive Glass-Containing Dentin Adhesive on the Permeability of Demineralized Dentin

Abstract

This study aimed to evaluate the effect of a novel bioactive glass (BAG)-containing dentin adhesive on the permeability of demineralized dentin. Bioactive glass (85% SiO2, 15% CaO) was fabricated using the sol-gel process, and two experimental dentin adhesives were prepared with 3 wt% silica (silica-containing dentin adhesive; SCA) or BAG (BAG-containing dentin adhesive; BCA). Micro-tensile bond strength (μTBS) test, fracture mode analysis, field-emission scanning electron microscopy (FE-SEM) analysis of adhesive and demineralized dentin, real-time dentinal fluid flow (DFF) rate measurement, and Raman confocal microscopy were performed to compare SCA and BCA. There was no difference in μTBS between the SCA and BCA (p > 0.05). Multiple precipitates were evident on the surface of the BCA, and partial occlusion of dentinal tubules was observed in FE-SEM of BCA-approximated dentin. The DFF rate was reduced by 50.10% after BCA approximation and increased by 6.54% after SCA approximation. Raman confocal spectroscopy revealed an increased intensity of the hydroxyapatite (HA) peak on the dentin surface after BCA application. The novel BAG-containing dentin adhesive showed the potential of both reducing dentin permeability and dentin remineralization.

Effect of Dentin Desensitizer Containing Novel Bioactive Glass on the Permeability of Dentin

“…In addition, the ∆DFF of those was higher than SNP group after two weeks of storage. The short-term effect of BAG on dentin permeability was also verified by Kim et al [12]. In their study, a BAG-containing dentin adhesive without an acidic functional monomer was used.…”

“…The reason was that the interface of the desensitizer-dentin was likely to deform or destruct during specimen preparation. Although this method has been used in other studies [12,14,46] and is also valid in this study, it still lacks clinical relevance. Cross-sectional interface analysis with hydraulic conductance should be necessary in a future study.…”

“…A total of 20 teeth randomly assigned to 4 experimental groups (n = 5) were used for the DFF rate measurements. The specimens were prepared as described in a previous study [12]. Briefly, the flat dentin surface was exposed, the root portion was removed 5 mm from the cemento-enamel junction, and the remaining pulp tissue was completely removed.…”

“…Thus, the supplementation of BAG into various dental restorative materials has been investigated. Dental composite resin or adhesive materials incorporating BAG have been reported to provide reduction of biofilm formation in pre-existing marginal gaps [8], remineralization of demineralized dentin [10][11][12], and prevention of demineralization and remineralization of enamel [13,14]. In addition, BAG showed ability to occlude dentinal tubule through the formation of HCA layer [15][16][17][18].…”

Bioactive Inorganic Materials for Dental Applications: A Narrative Review

“…It also enhanced the tooth enamel remineralization process due to its bioactivity [ 187 ]. In other studies, bioactive glass-based adhesives were applied to demineralized dentin, and dentin was observed to remineralize successfully [ 188 , 189 , 190 ]. Sauro et al [ 191 ] probed the bonded-dentin interface healing response to applying pure Bioglass 45S5 and Zinc-polycarboxylated bioactive glass-based dental composite.…”