Effect of non-steroidal anti-inflammatory drugs on pulpal and periapical inflammation induced by lipopolysaccharide

Ribeiro-Santos, Fernanda Regina; Arnez, Maya Fernanda Manfrin; Carvalho, Marcio Santos de; Politi, Marília Pacífico Lucisano; Queiroz, Alexandra Mussolino de; Nelson‐Filho, Paulo; Silva, Léa Assed Bezerra da; Faccioli, Lúcia Helena; Paula-Silva, Francisco Wanderley Garcia

doi:10.1007/s00784-021-03919-3

Cited by 7 publications

(12 citation statements)

References 34 publications

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“…Previous investigations have reported that at 28 days, periapical bone resorption occurs after inoculation of 10 µL of LPS suspension [10,11]. Therefore, in the present study, the period of 30 days after inoculation of LPS was investigated, which meant that apical periodontitis was established prior to the treatment with MK-886.…”

Section: Discussionmentioning

confidence: 99%

“…with 10% ketamine hydrochloride (150 mg/kg; National Pharmaceutical Chemistry Union Agener S/A, Embu-Guaçu, SP, Brazil) and xylazine (7.5 mg/kg; Dopaser, Labs Calier S/A, Barcelona, Spain). Anaesthesia was sustained during the operative procedures throughout the experimental period, and the animals were monitored by a veterinarian throughout the entire experimental period as previously described [10,11].…”

Section: Animalsmentioning

confidence: 99%

“…RNA extraction was performed with RNeasy Mini kit (RNeasy ® Mini, Qiagen Inc., Valencia, CA, USA) and samples were treated with DNAse I (RNase-Free DNase Set; Qiagen Inc.), according to manufacturer's protocol. RNA integrity was analyzed using 1% agarose electrophoresis and quantity was estimated in NanoDrop 1000 (Thermo Fisher Scienti c Inc., Wilmington, DE, USA) at 230, 260 and 280 ηm wavelengths [11].…”

Section: Quantitative Reverse Transcriptase-polymerase Chain Reaction...mentioning

confidence: 99%

“…In this sense, considering that AP is a multifactorial disease, to reduce failure in the NSRCT approaches that address microbiology, quality of treatment and host response particularities are needed [7]. Thus, recent research investigate coadjutant therapies to limit the AP development and establishment, or improve their healing rates after NSRCT, with promising results through the use of anti-in ammatory drugs and probiotics via systemic or topical administration, in order to control the in ammatory process during AP establishment and repair [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

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Systemic inhibition of 5-lipoxygenase by MK-886 exacerbates apical periodontitis bone loss in a mouse model

Petean

Sousa

Silva

et al. 2022

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Background: To investigate if 5-LO selective inhibitor (MK-886) could be used for systemic treatment of experimentally induced apical periodontitis in a mouse model. Methods: Twenty-four C57BL / 6 mice were used. After coronal opening, a solution containing E. coli LPS (1.0µg / µl) was inoculated into the root canals of the lower and upper right first molars (n= 72 teeth). After 30 days apical periodontitis was established, and the animals were treated with MK-886 (5 mg / kg), a 5-LO inhibitor, for 7 and 14 days. The tissues were removed for histopathological and histometric analyses, evaluation of osteoclast number and gene expression for RANK (Tnfrsf11a), RANKL (Tnfsf11), OPG (Tnfrsf11b), TRAP (Acp5), matrix metalloproteinase-9 (Mmp9), cathepsin K (Ctsk) and calcitonin receptor (Calcr). Statistical data analysis was performed using Kruskal Wallis followed by Dunn's tests (α = 0.05). Results: Administration of MK-886 for 7 days exerted no effect on apical periodontitis expansion compared to LPS inoculation without treatment (p = 0.3549), while treatment for 14 days exacerbated bone loss (p < 0.0001). Administration of MK-886 enhanced osteoclastogenesis signaling and osteoclast formation within 7 days (p = 0.0005), but no effect at 14 days (p > 0.9999). After 7 days of treatment, MK-886 induced mRNA expression for Acp5 (p = 0.0001), Calcr (p = 0.0003), Mmp9 (p = 0.0005) and Ctsk (p = 0.0008), however no effect in those gene expression was observed after 14 days (p > 0.05). Conclusion: Systemic treatment with MK-886 exacerbated LPS-induced apical periodontitis in a mouse model.

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Section: Discussionmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

Section: Quantitative Reverse Transcriptase-polymerase Chain Reaction...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Systemic inhibition of 5-lipoxygenase by MK-886 exacerbates apical periodontitis bone loss in a mouse model

Petean

Sousa

Silva

et al. 2022

Preprint

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“…Because AP is a host tissue inflammatory response to bacterial infection of the root canal and serves the purpose of restraining the progression of infection towards bone tissue, systemic therapy would be useful in cases of difficult healing apical periodontitis such as lesions in patients with comorbidities or dysbiosis. Thus, recent research investigates coadjutant therapies to limit the AP development and establishment, or improve their healing rates after NSRCT, with promising results through the use of anti-inflammatory drugs and probiotics via systemic or topical administration, in order to control the inflammatory process during AP establishment and repair [ 8 – 11 ].…”

Section: Introductionmentioning

confidence: 99%

Systemic inhibition of 5-lipoxygenase by MK-886 exacerbates apical periodontitis bone loss in a mouse model

et al. 2023

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Background To investigate if 5-LO selective inhibitor (MK-886) could be used for systemic treatment of experimentally induced apical periodontitis in a mouse model. Methods Twenty-four C57BL/6 mice were used. After coronal opening, a solution containing Escherichiacoli LPS (1.0 µg/µL) was inoculated into the root canals of the lower and upper right first molars (n = 72 teeth). After 30 days apical periodontitis was established, and the animals were treated with MK-886 (5 mg/kg), a 5-LO inhibitor, for 7 and 14 days. The tissues were removed for histopathological and histometric analyses, evaluation of osteoclast number and gene expression for receptor activator of nuclear factor kappa-B (Tnfrsf11a), receptor activator of nuclear factor kappa-B ligand (Tnfsf11), osteoprotegerin (Tnfrsf11b), tartrate-resistant acid phosphatase (Acp5), matrix metalloproteinase-9 (Mmp9), cathepsin K (Ctsk) and calcitonin receptor (Calcr). Statistical data analysis was performed using Kruskal Wallis followed by Dunn’s tests (α = 0.05). Results Administration of MK-886 for 7 days exerted no effect on apical periodontitis progression compared to LPS inoculation without treatment (p = 0.3549), while treatment for 14 days exacerbated bone loss (p < 0.0001). Administration of MK-886 enhanced osteoclastogenesis signaling and osteoclast formation within 7 days (p = 0.0005), but exerted no effect at 14 days (p > 0.9999). After 7 days of treatment, MK-886 induced mRNA expression for Acp5 (p = 0.0001), Calcr (p = 0.0003), Mmp9 (p = 0.0005) and Ctsk (p = 0.0008), however no effect in those gene expression was observed after 14 days (p > 0.05). Conclusion Systemic treatment with MK-886 exacerbated LPS-induced apical periodontitis in a mouse model.

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A sinalização tnf-alfa-tnfr1 regula diferencialmente a perda óssea periapical e a neoformação dentinária em modelo experimental murino

Almeida Júnior

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que estão presentes em minhas orações. Que nesta trajetória me abençoaram e continuam me abençoando sempre. A fé me permitiu chegar até este momento. Na alegria e tristeza, as orações que me fazem sentir a presença do divino em minha vida. Aos meus pais, Eliane Esteves e Luciano Aparecido de Almeida, pelo apoio incondicional das minhas escolhas, carinho, amor, companheirismo. A união entre a família é o mais sagrado aqui na Terra. Fico muito feliz por vocês serem meu porto seguro, minha razão pela qual estou conseguindo alcançar meus objetivos. Mesmo em outra cidade, continuamos unidos. Muito obrigado por tudo que fizeram e continuam fazendo por mim. Aos meus avôs Vicente de Paula Esteves (in memoriam) e Manoel Messias de Almeida (in memoriam), e minhas avós Maria Zélia Tercetti Esteves e Maria José Cézar de Almeida por todas as orações, torcida e dedicação na criação do seu neto.Às crianças e pacientes, por me fazer acreditar na pureza do ser humano e me despertar a cada dia mais a busca por conhecimento na área da Odontopediatria. Dedico esta conquista a todos vocês! Muito obrigado! AGRADECIMENTOS ESPECIAISAgradeço ao Prof. Dr. Francisco Wanderley Garcia de Paula e Silva, por ter me acolhido como seu orientado e me ensinado tantas coisas na vida profissional e pessoal.Não tenho palavras para descrever a gratidão que tenho. Obrigado professor, pela sua atenção, dedicação, paciência, amor pela profissão e seus ensinamentos. À Prof.ª Dra. Raquel Assed Bezerra Segato, coordenadora do programa de Pós-Graduação em Odontopediatria da FORP-USP, que me recepcionou de maneira afetuosa nesta instituição.

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Effect of non-steroidal anti-inflammatory drugs on pulpal and periapical inflammation induced by lipopolysaccharide

Cited by 7 publications

References 34 publications

Systemic inhibition of 5-lipoxygenase by MK-886 exacerbates apical periodontitis bone loss in a mouse model

Systemic inhibition of 5-lipoxygenase by MK-886 exacerbates apical periodontitis bone loss in a mouse model

Systemic inhibition of 5-lipoxygenase by MK-886 exacerbates apical periodontitis bone loss in a mouse model

A sinalização tnf-alfa-tnfr1 regula diferencialmente a perda óssea periapical e a neoformação dentinária em modelo experimental murino

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