Effect of non-Newtonian fluid properties on bovine sperm motility

Hyakutake, Toru; Suzuki, Hiroki; Yamamoto, Satoru

doi:10.1016/j.jbiomech.2015.08.005

Cited by 31 publications

(31 citation statements)

References 40 publications

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“…Thus, the averaged viscosity of mouse uterus and oviduct is 81 and 1.8 cP respectively (Miki & Clapham 2013), in the range of viscosity obtained with the percentages of PVP (Hyakutake et al 2015) used in our study. The effects of viscosity on mouse sperm kinetics observed here including a reduced velocity but more regular trajectory are in agreement with observations in hamster (Suarez et al 1991), mice (Suarez & Dai 1992) and cattle ( Hyakutake et al 2015). The different behaviour shown by spermatozoa in low-viscous compared with viscous media (Fig.…”

Section: Discussionmentioning

confidence: 56%

“…Complementary studies would include analyses of the response to P 4 under other rheological conditions of the surrounding medium such as the viscoelasticity that characterize some fluids present along the female genital tract (Suarez & Dai 1992). It has been reported that at similar viscosity, the VSL and flagellar beat frequency of cattle spermatozoa are higher in non-Newtonian fluids leading to a more linear motility type (Hyakutake et al 2015) compared with Newtonian fluids. Accordingly, it has been shown that under viscoelastic conditions, hyperactive samples of mouse spermatozoa linearize the motility, whereas non-hyperactive spermatozoa behave in an opposite way (Suarez & Dai 1992).…”

Section: Discussionmentioning

confidence: 99%

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Progesterone effects on mouse sperm kinetics in conditions of viscosity

2016

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The spermatozoa delivered to the female genital tract need to swim towards the oocyte through viscous secretions. Once close to the oocyte, the spermatozoa are guided by a gradient of progesterone (P 4 ) and other unknown chemoattractants via a process known as chemotaxis. Using polyvinylpyrrolidone to establish the conditions of viscosity, we examined the response of mouse spermatozoa to P 4 . Herein, we show that in low-viscous media, P 4 induces hyperactive-like motility whereby sperm show erratic trajectories and non-progressive movement. However, an opposite response is produced in viscous medium in that trajectories are linear and motility is more progressive and less erratic. Our observations provide a behavioural explanation for the chemotaxis of spermatozoa swimming under viscous conditions in a spatial gradient of the chemoattractant P 4 . They also highlight the importance of using viscous solutions to mimic in vivo conditions when analysing sperm behaviour in response to any stimulus.Reproduction (2016) 151 501-507

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Progesterone effects on mouse sperm kinetics in conditions of viscosity

2016

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“…Adding 1% methyl cellulose to a conventional assay medium results in viscosity values several orders of magnitude higher (at shear rate 5 s −1 ) 24,25 . This viscosity mimics that of fluids of the reproductive tract, reported to be 0.1-1.0 Pa s 30 and has previously been used to study sperm motion in viscous environment similar to the in vivo conditions 31–34 .…”

Section: Resultsmentioning

confidence: 99%

The swim-up technique separates bovine sperm by metabolic rates, motility and tail length

Magdanz¹,

Boryshpolets

Ridzewski³

et al. 2019

Preprint

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18Swim-up is a sperm purification method that is being used daily in andrology labs around the 19 world as a simple step for in vitro sperm selection. This method accumulates the most motile 20 sperm in the upper fraction and leaves sperm with low or no motility in the lower fraction but 21 the underlying reasons are not fully understood. In this article, we compare metabolic rate, 22 motility and sperm tail length of bovine sperm cells of the upper and lower fraction. The 23 metabolic assay platform reveals oxygen consumption rates and extracellular acidification rates 24 simultaneously and thereby delivers the metabolic rates in real time. Our study confirms the 25 upper fraction of bull sperm has improved motility compared to the cells in the lower fraction 26 and shows higher metabolic rates. This pattern was consistent across media of two different 27 levels of viscosity. Sperm with longer flagella are selected in the upper fraction. We conclude 28 that the motility-based separation of the swim-up technique is based on metabolic differences. 29Metabolic assays could serve as additional or alternative, label-free method to evaluate sperm 30 quality, which is likely particularly useful in cases of asthenozoospermia and teratospermia. 31Furthermore, metabolic measurements of sperm cells can reveal differences in metabolic 32 pathways in different environments. 33 2 1 Keywords 2 swim-up, bovine sperm, metabolism, sperm selection, sperm quality 3

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“…properties of mucus on sperm motility (Hyakutake, et al 2015b). Additionally, the microgrooves of the bovine cervical wall may influence sperm migration (Suarez, 2016).…”

Section: Discussionmentioning

confidence: 99%

Experimental study on the effect of a surrounding fluid on bovine sperm motility in three dimensions

Hyakutake

ORIHARA

Mezaki

2017

JBSE

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We constructed an experimental system that can observe bovine sperm motility in three dimensions, simultaneously from the vertical and horizontal directions. The comparison of the experiments conducted using cover glass and square tubing indicates that the effect of the cover glass on the sperm motility is not negligible. Based on this result, we investigated a three-dimensional trajectory of a motile sperm in the square tubing by simultaneously observing through two high-speed cameras. In addition, we investigated the effect of different viscosities of the surrounding fluid on the sperm motility. The experimental results indicate that the increasing viscosity causes a decline in the sperm motility. Furthermore, we focus on the width of the sperm head and the flagellar shape. At low viscosity, the time variation of the head widths is relevant with the two directions and varies periodically. In contrast, at high viscosity, an almost constant value is maintained and periodical time variations are not observed for both directions. Moreover, a difference in the viscosity is observed for the tortuosity of the flagellum shape. These results suggest that the three-dimensional flagellar structure of the bovine sperm is a helix whose cross-sectional shape is an ellipse with quite a large aspect ratio. Further, the results state whether the rotation or nonrotation of the sperm is dependent on the balance between the viscous resistance acting on the sperm and the torque due to the flagellar shape. The experimental results obtained will be useful in clarifying the mechanics of sperm motility under their actual environmental conditions at high viscosity.

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Effect of non-Newtonian fluid properties on bovine sperm motility

Cited by 31 publications

References 40 publications

Progesterone effects on mouse sperm kinetics in conditions of viscosity

Progesterone effects on mouse sperm kinetics in conditions of viscosity

The swim-up technique separates bovine sperm by metabolic rates, motility and tail length

Experimental study on the effect of a surrounding fluid on bovine sperm motility in three dimensions

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