The swim-up technique separates bovine sperm by metabolic rates, motility and tail length

Magdanz,; Boryshpolets, Sergii; Ridzewski, Clara; Eckel, Barbara; Reinhardt, Klaus

doi:10.1101/624502

Cited by 6 publications

(3 citation statements)

References 48 publications

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“…Effects of MitoQ on oxygen consumption rate of other cell types are controversial, ranging from no influence (Gottwald et al., 2018 ) to impairment of oxidative phosphorylation (Pokrzywinski et al., 2016 ; Sun et al., 2017 ). The lack of influence of MitoQ on sperm kinematics observed in our study could be explained by the capacity of bovine sperm to produce ATP using both anaerobic (glycolytic) and aerobic (oxidative phosphorylation) pathways (Losano et al., 2017 ; Magdanz et al., 2019 ).…”

Section: Discussionmentioning

confidence: 53%

Mitoquinone does not improve sperm cryo‐resistance in bulls

Câmara

Ibănescu

Siuda

et al. 2021

Reprod Domestic Animals

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Artificial insemination is a cattle breeding method implemented worldwide. Therefore, advances in bull sperm cryopreservation and subsequent fertility are a critical economic trait for breeding programmes (Grötter et al., 2019;Hitit et al., 2020). Increasing sperm cryosurvival persists as a major challenge, prompting ongoing research on new cryopreservation techniques and the addition of several constituents to semen extender (Chatterjee & Gagnon, 2001;Peris-Frau et al., 2020).Production of reactive oxygen species (ROS) and the subsequent generation of controlled oxidative stress (OS) are important to several physiological pathways of sperm (Bollwein & Bittner, 2018;Moraes & Meyers, 2018). However, OS associated with sperm cryopreservation can reduce sperm quality (Gibb et al., 2020;Hitit et al., 2020).Sperm alterations induced by OS include membrane changes (fluidity, lipoperoxidation and ion channel dysfunction), reduced motility, DNA damage, mitochondrial electron transport disruption, and lower in-vitro and in-vivo fertility (

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Section: Discussionmentioning

confidence: 53%

Mitoquinone does not improve sperm cryo‐resistance in bulls

Câmara

Ibănescu

Siuda

et al. 2021

Reprod Domestic Animals

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“…These results allowed us to validate the robustness of the technique, and to calculate, for the first time in mouse sperm, a range of relevant metabolic parameters that allow for a comparison between studies and species. Importantly, this is, to the best of our knowledge, the first study to achieve robust metabolic measurements using XF EFA technology in freely moving sperm, since previous work using these techniques Frontiers in Cell and Developmental Biology frontiersin.org necessitated the use of attached cells (Tourmente et al, 2015a;Magdanz et al, 2019;Balbach et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Capacitation promotes a shift in energy metabolism in murine sperm

Tourmente

Sansegundo

Rial

et al. 2022

Front. Cell Dev. Biol.

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In mammals, sperm acquire fertilization ability after a series of physiological and biochemical changes, collectively known as capacitation, that occur inside the female reproductive tract. In addition to other requirements, sperm bioenergetic metabolism has been identified as a fundamental component in the acquisition of capacitation. Mammalian sperm produce ATP through two main metabolic processes, oxidative phosphorylation (OXPHOS) and aerobic glycolysis that are localized to two different flagellar compartments, the midpiece, and the principal piece, respectively. In mouse sperm, the occurrence of many events associated with capacitation relies on the activity of these two energy-producing pathways, leading to the hypothesis that some of these events may impose changes in sperm energetic demands. In the present study, we used extracellular flux analysis to evaluate changes in glycolytic and respiratory parameters of murine sperm that occur as a consequence of capacitation. Furthermore, we examined whether these variations affect sperm ATP sustainability. Our results show that capacitation promotes a shift in the usage ratio of the two main metabolic pathways, from oxidative to glycolytic. However, this metabolic rewiring does not seem to affect the rate at which the sperm consume ATP. We conclude that the probable function of the metabolic switch is to increase the ATP supply in the distal flagellar regions, thus sustaining the energetic demands that arise from capacitation.

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“…To collect 165 sperm, we agitated the tissue at 300rpm (ThermoMixer F1.5, Eppendorf, Germany) at 37ºC for ten 166 minutes, inverting the tube at the five-and ten-minute mark, then incubated the tissue undisturbed 167 for two minutes. Using pipette tips cut to create a wider opening, we collected live sperm cells for 168 analysis from just below the meniscus of the solution to enrich for the most motile sperm 169 (Magdanz et al 2019). Next we estimated sperm density using a computer-assisted sperm analysis…”

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confidence: 99%

Post-copulatory sexual selection is associated with sperm aggregate quality in Peromyscus mice

Hook

Weber

Fisher

2020

Preprint

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Sperm of some species form motile, coordinated groups as they migrate through the female 49 reproductive tract to the site of fertilization. This collective motion is predicted to improve sperm 50 swimming performance and therefore may be beneficial in a competitive context, but limited 51 evidence supports this theory. Here we examine sperm aggregates across closely-related species of 52 Peromyscus mice that naturally vary by mating system, and thus sperm competition intensity. We 53 find that phylogenetic history predicts the likelihood that sperm will aggregate, and that relative 54 testis size is negatively associated with variation in number of aggregated cells, suggesting that 55 sperm competition has a stabilizing effect on sperm group size. Moreover, we show that 56 aggregates are kinematically beneficial for some species but costly for others, and these 57 differences are largely dependent on the orientation and composition of sperm within the groups. 58In addition, when we compared sperm of the two sister-species that aggregate most frequently, we 59 find that sperm from the species that evolved under intense sperm competition forms aggregates 60 with more efficient geometry more frequently than sperm from its monogamous congener. These 61 results are consistent with the prediction that sperm aggregation evolved to improve motility in a 62 competitive context; however, when monogamy evolved secondarily, relaxed sexual selection 63 allowed for less efficient strategies to persist. Together, our findings in Peromyscus reveal that 64 collective sperm behavior is likely to evolve rapidly and is shaped by changes in the selective 65 regime. 66 67 widely across taxa (7). In mammals alone, sperm of some species assemble during epididymal 100 transport and are molecularly "glued" to one another as bi-flagellate pairs in grey short-tailed 101 opossums (25), as bundles of roughly 100 cells in monotremes (26), or as organized rouleaux of 102 five or more cells in guinea pigs (Cavia porcellus, 24). Conversely, mammalian sperm may also 103 assemble after ejaculation to form variably-sized groups. For instance, sperm may form temporary 104 clusters of up to sixteen cells in bulls (Bos Taurus, 15) or more fixed groups of up to 30 cells in 105 house mice (5), 50 cells in the Norway rat (5), or thousands of cells in the wood mouse (Apodemus 106 sylvaticus, 4) whereby the hook-shaped heads interlock or attach to the flagella of other sperm. For 107 these latter species in which sperm groups form after ejaculation, both single and aggregated 108 sperm typically co-occur, thus allowing for direct comparisons between collective and solitary 109 sperm movements within an ejaculate while controlling for within-male variability. 110Closely-related species in the rodent genus, Peromyscus, produce sperm that naturally vary in 111 their collective behavior. Sperm of some species assemble temporary groups after ejaculation by 112 adhering to one another at their head region (27) and disassemble prior to fertilization (10). In P. 1...

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The swim-up technique separates bovine sperm by metabolic rates, motility and tail length

Cited by 6 publications

References 48 publications

Mitoquinone does not improve sperm cryo‐resistance in bulls

Mitoquinone does not improve sperm cryo‐resistance in bulls

Capacitation promotes a shift in energy metabolism in murine sperm

Post-copulatory sexual selection is associated with sperm aggregate quality in Peromyscus mice

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