Effect of non-genetic factors on paraoxonase 1 activity in patients undergoing hemodialysis

Rajković, Marija Grdić; Rumora, Lada; Juretić, Dubravka; Grubišić, Tihana Žanić; Flegar-Meštrić, Zlata; Vrkić, Nada; Sinjeri, Željko; Barišić, Karmela

doi:10.1016/j.clinbiochem.2010.08.024

Cited by 21 publications

(22 citation statements)

References 38 publications

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“…According to Gugliucci and colleagues, these results suggest another type of mechanism related to uremic toxins retention that possibly causes the Journal of Biophysical Chemistry impairment of HDL's PON-1 antioxidant function [3]. Rajković et al [20] also found a reduced PON-1 activity in patients with ESRD when replicating the in vitro experiments performed by Gugliucci et al [3]. The obtained results show that high serum concentrations of urea, creatinine and uric acid had no impact on PON-1 activity.…”

Section: Discussionmentioning

confidence: 99%

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Low-Flow Polysulfone Hemodialysis Alters Lipoprotein Parameters, Paraoxonase Activity and <i>in Vitro</i> Incorporation of Phospholipids

Marques¹,

Silva²,

Mattoso³

et al. 2017

JBPC

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End stage-renal-disease (ESRD) is associated with dyslipidemia and premature atherosclerosis. The study evaluates the effect of hemodialysis (HD) on HDL-remodeling between pre-and post-HD. Was conducted a cross-sectional study with 50 ESRD male patients, undergoing HD at Ana Neri Hospital, Salvador, Brazil. All individuals were on HD for at least 3 months, into a three sessions protocol for 3 -4 hours per week, with a polysulfone low-flow basicdialyzing-membrane and unfractionated-heparin. HDL Phospholipid-incorporation was measured by 14 C-PL-scintillation-counting, expressed as % 14 C-PL/mL/hour. Paraoxonase (PON-1) activity was measured by spectrophotometry using paraoxon as substrate. Cardiovascular risk ratios and atherogenic index of plasma were calculated. Total cholesterol, HDL-C and non-HDL-C increased at post-HD on all age groups, but without triglycerides (TG) changes. TG/HDL-C decreased in 30 -39 and 40 -49 year (y) at post-HD (p < 0.05). LDL-C/apoB increased in >60 y, after HD (p < 0.05). HDL-C/apo-AI increased in 40 -49 y (p < 0. C-PL-incorporation was reduced in >60 y (p < 0.05), when compared to pre-and post-HD. ESRD patients undergoing HD shows important changes on lipid-profile, PON-1-activity, cardiac risk ratios and HDL-remodeling. These results demonstrate the influence of HD with a polysulfone low-flow basic-dialyzing-membrane and unfractionated-heparin on lipoprotein metabolism.

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Section: Discussionmentioning

confidence: 99%

“…PON-1 activity has been cited as the major antioxidant component of HDL [10], and the loss of its reductive capacity has been noticed on HD patients as a contributing factor for atherosclerosis development [20]. PON-1 is mentioned to be present at various steps of systemic oxidative stress, both in human and animal models [21].…”

Section: Discussionmentioning

confidence: 99%

Low-Flow Polysulfone Hemodialysis Alters Lipoprotein Parameters, Paraoxonase Activity and <i>in Vitro</i> Incorporation of Phospholipids

Marques¹,

Silva²,

Mattoso³

et al. 2017

JBPC

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“…ApoA1, the major protein in HDL, stabilizes PON1, and binds it with a very high affinity [9,10]. In recent years, interactions between drugs and human paraoxonase interested many researchers that have tested different xenobiotics effects in vitro on PON1 [11]. Antibiotics including ceftriaxone, ceftazidime and sulfonamides and furosemide, were confirmed to decrease the in vitro enzyme activity at different concentrations [12,13].…”

Section: Discussionmentioning

confidence: 99%

In vitro Effects of Salicylic Acid on Plasma Paraoxonase 1 Activity

Mouhamed¹

2013

J Drug Metab Toxicol

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Objectives: Our study aimed to examine the effects of salicylic acid on paraoxonase activity in healthy volunteers. Methods: Plasma from healthy volunteers was spiked with salicylic acid drug. The working solutions were then diluted with plasma to obtain concentrations that covered the therapeutic margin. Specimens were incubated at 25 and 37°C, and we measured PON1 activities in the presence of different drug concentrations after 1 h, 3 h, and 24 h and after one week.Results: After incubation in vitro, a significant increase of paraoxonase 1 activity according to salicylic acid concentration and incubation duration, regardless of temperature incubation was showed. This increase was marked especially after 24 h of incubation. The stimulatory effect of salicylic acid is concentration and incubation durationdependent and does not seem to be related to temperature incubation. Conclusion:The tested Salicylic acid significantly stimulated PON1 activity in a concentration dependent manner. This result shows that acetyl salicylic acid treatments have a beneficial effect dose and time dependent that may contribute to prevent atherosclerosis.

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“…PON1 activity also declines in schizophrenic conditions (104 -105). It was noted that PON1 acti vity decreased in patients with chronic renal failure (106)(107)(108)(109)(110) and that it was restored following kidney transplantation (106).…”

Section: Clinical Significancementioning

confidence: 99%

The Past and Present of Paraoxonase Enzyme: Its Role in the Cardiovascular System and Some Diseases

Aydın¹,

Şahin²,

Aydın³

et al. 2012

Journal of Medical Biochemistry

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Summary: Although paraoxonase is synthesized in many tissues including the heart, colon, kidneys, lungs, small intestines and brain, its major locus of synthesis is the liver. PON1 is in close association with apolipoproteins and protects LDL against oxidation. It was reported that PON1 quantities dropped to 40 times lower than normal in cardiovascular diseases and diseases like diabetes, ulcerative colitis, Crohn's disease, chronic renal failure, SLE, Behcet's disease, cancer, hepatitis B, obesity, metabolic syndrome, Alzheimer's and dementia. It is speculated that the concerning decline in serum PON1 amount results from single nucleotide polymorphism in the coding (Q192R, L55M) and promoter (T-108C) sites of the PON1 gene. Additionally, circulating amounts of PON1 are affected by vitamins, antioxidants, fatty acids, dietary factors, drugs, age and lifestyle. This collection attempts to review and examine the past and present studies of paraoxonase and its relation with the cardiovascular system and some relevant diseases.Keywords: paraoxonase, polymorphism, cardiovascular system diseases Kratak sadr`aj: Iako se paraoksonaza sinteti{e u mnogim tkivima, uklju~uju}i srce, debelo crevo, bubrege, plu}a, tanko crevo i mozak, glavno mesto njene sinteze je jetra. PON1 je blisko povezana sa apolipoproteinima i {titi LDL od oksidacije. Koli~ine PON1 ~ak 40 puta ni`e od normalnog nivoa zabele`ene su u kardiovaskularnim bolestima i oboljenjima kao {to su dijabetes, ulcerozni kolitis, Kronova bolest, hroni~na bubre`na insuficijencija, sistemski eritemski lupus, Beh~etov sindrom, kancer, hepatitis B, gojaznost, metaboli~ki sindrom, Alchajmerova bolest i demencija. Spekuli{e se da pomenuti pad nivoa PON1 u serumu poti~e od polimorfizma pojedina~nih nukleotida na kodnim (Q192R, L55M) i promoterskim (T-108C) podru~jima gena PON1. Pored toga, na koli~inu PON1 u cirkulaciji uti~u vita mini, antioksidansi, masne kiseline, faktori ishra ne, le kovi, godine i na~in `ivota. Ovaj pregled predstavlja poku{aj da se izlo`e i razmotre pro{le i sada{nje studije o paraoksonazi i njenom odnosu sa kardiovaskularnim sistemom i nekim relevantnim bolestima.

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Effect of non-genetic factors on paraoxonase 1 activity in patients undergoing hemodialysis

Cited by 21 publications

References 38 publications

Low-Flow Polysulfone Hemodialysis Alters Lipoprotein Parameters, Paraoxonase Activity and <i>in Vitro</i> Incorporation of Phospholipids

Low-Flow Polysulfone Hemodialysis Alters Lipoprotein Parameters, Paraoxonase Activity and <i>in Vitro</i> Incorporation of Phospholipids

In vitro Effects of Salicylic Acid on Plasma Paraoxonase 1 Activity

The Past and Present of Paraoxonase Enzyme: Its Role in the Cardiovascular System and Some Diseases

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Effect of non-genetic factors on paraoxonase 1 activity in patients undergoing hemodialysis

Cited by 21 publications

References 38 publications

Low-Flow Polysulfone Hemodialysis Alters Lipoprotein Parameters, Paraoxonase Activity and &lt;i&gt;in Vitro&lt;/i&gt; Incorporation of Phospholipids

Low-Flow Polysulfone Hemodialysis Alters Lipoprotein Parameters, Paraoxonase Activity and &lt;i&gt;in Vitro&lt;/i&gt; Incorporation of Phospholipids

In vitro Effects of Salicylic Acid on Plasma Paraoxonase 1 Activity

The Past and Present of Paraoxonase Enzyme: Its Role in the Cardiovascular System and Some Diseases

Contact Info

Product

Resources

About

Low-Flow Polysulfone Hemodialysis Alters Lipoprotein Parameters, Paraoxonase Activity and <i>in Vitro</i> Incorporation of Phospholipids

Low-Flow Polysulfone Hemodialysis Alters Lipoprotein Parameters, Paraoxonase Activity and <i>in Vitro</i> Incorporation of Phospholipids