Effect of Nilvadipine on adrenocortical cell proliferation and steroidogenesis in early stage of adrenal regeneration

Zieleniewski, Wojciech

doi:10.1016/s0940-2993(11)80400-8

Cited by 5 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, it has been postulated that a G-protein may modulate the activity of calcium channels and thus alter intracellular calcium concentration (Bergamaschi et al, 1990). This mechanism should strongly corroborate with our previous finding that nilvadipine, a calcium channel antagonist, inhibits cell growth and steroidogenic capacity of regenerating adrenal cortex (Zieleniewski, 1993).…”

Section: Discussionsupporting

confidence: 87%

Effect of interleukin-la, IL-lb and IL-1 receptor antibody on the proliferation and steroidogenesis of regenerating rat adrenal cortex

Zieleniewski

Zieleniewski²,

Stępień³

2009

Exp Clin Endocrinol Diabetes

View full text Add to dashboard Cite

Several data suggest that the endocrine and immune systems are closely interconnected, therefore, we investigated the effect of interleukin-1 on adrenocortical regeneration. The study was performed on adult rats subjected to left adrenal enucleation combined with contralateral adrenalectomy. The animals were given purified human recombinant IL-1 alpha (5 micrograms/kg), IL-1 beta (5 micrograms/kg) anti-human IL-1 receptor antibody (10 micrograms/kg) and combination of interleukins and antibodies. The cell proliferation measured by bromodeoxyuridine incorporation and plasma corticosterone determined by a RIA kit were evaluated six days after operation. The mean cell growth ratio expressed as number of BrdU-positive cells per 1,000 cells was 11.86 +/- 0.45 in the control group. IL-1b significantly stimulated the proliferation ratio (15.30 +/- 0.88; p < 0.01) and this effect could be prevented by addition of IL-1rAb. The antibody caused also a well-marked inhibition of cell growth ratio (8.23 +/- 0.67; p < 0.01). The plasma corticosterone concentration was higher in IL-1b-treated animals than in the control group, 107.79 +/- 2.82 vs. 61.08 +/- 2.0 ng/ml, respectively, p < 0.01. On the other hand, IL-1rAb decreased corticosterone secretion (31.53 +/- 2.58 ng/ml, p < 0.01) and reversed the stimulatory effect of of IL-1b. In contrast, IL-1a seemed to have no influence on adrenocortical regeneration. In conclusion, this study supported the thesis of close interconnection between immune and endocrine systems and revealed that IL-1b could act as a growth-promoting factor on regenerating adrenal cortex in the rat.

show abstract

Section: Discussionsupporting

confidence: 87%

Effect of interleukin-la, IL-lb and IL-1 receptor antibody on the proliferation and steroidogenesis of regenerating rat adrenal cortex

Zieleniewski

Zieleniewski²,

Stępień³

2009

Exp Clin Endocrinol Diabetes

View full text Add to dashboard Cite

show abstract

“…On the other hand, it is believed that cells require calcium ions during both the S phase and mitosis (11). The presence in the adrenal cortex of voltage-dependent calcium channels sensitive to dihydropyridines has been clearly demonstrated (12,13).…”

Section: Discussionmentioning

confidence: 99%

The stimulatory effect of endothelin-1 on regenerating adrenal cortex is reversed by nifedipine

Zieleniewski¹

1997

European Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

Endothelin-1 (ET-1), a potent vasoconstrictor, was found to act in non-vascular tissues, for example it enhanced aldosterone output from adrenal zona glomerulosa. As the adrenal cortex is capable of regeneration after enucleation, it seemed of interest to study the effects of ET-1 on adrenocortical regeneration. The study was performed on adult rats subjected to left adrenal enucleation combined with contralateral adrenalectomy. Mitotic index was employed to assess the proliferation of regenerating adrenal cortex cells. Plasma corticosterone was measured by a standard RIA kit. ET-1 significantly raised the mitotic index of regenerating rat adrenal cortex by six days after surgery. On the other hand, nifedipine reduced the proliferation ratio and abolished the stimulatory influence of ET-1. Similarly, ET-1 enhanced corticosterone output from the regenerating adrenal cortex, and this could be prevented by the addition of nifedipine. This study has shown that ET-1 might act as a regulatory factor on the regenerating adrenal cortex via calcium channels.

show abstract

Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part II: Oncology, chemotherapy and carcinogenesis

Dolbeare¹

1995

Histochem J

View full text Add to dashboard Cite

show abstract

Effect of Nilvadipine on adrenocortical cell proliferation and steroidogenesis in early stage of adrenal regeneration

Cited by 5 publications

References 22 publications

Effect of interleukin-la, IL-lb and IL-1 receptor antibody on the proliferation and steroidogenesis of regenerating rat adrenal cortex

Effect of interleukin-la, IL-lb and IL-1 receptor antibody on the proliferation and steroidogenesis of regenerating rat adrenal cortex

The stimulatory effect of endothelin-1 on regenerating adrenal cortex is reversed by nifedipine

Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part II: Oncology, chemotherapy and carcinogenesis

Contact Info

Product

Resources

About