Effect of Nigella sativa L. Seed on the Kidney of Monosodium Glutamate Challenged Rats

Abstract

Monosodium glutamate (MSG) consumption is responsible for a wide spectrum of health hazards including nephrotoxicity. The search for phytochemical strategies having broad safety profile to counter MSG toxicity is worthwhile. Nigella sativa L. seed (NSS) is very promising in this regard owing to its antioxidant and cytoprotective nature. Therefore, we attempted to investigate the potential protective effect of NSS on MSG-induced renal toxicity in rats. To accomplish this objective, fifteen adult Wistar albino rats were randomly and equally divided into three groups for 21 days: the control group received no treatment, MSG group supplemented with MSG at a dose of 30 g/kg feed, and MSG + NSS group supplemented with MSG at the same previous dose in conjugation with NSS at a dose of 30 g/kg feed. MSG and its combination with NSS failed to cause any significant difference in the kidney function parameters in comparison with the control. A significant elevation in lipid peroxides (LPO) level, glutathione-S-transferase activity and total antioxidant capacity (TAC) and a significant reduction in superoxide dismutase activity were found in MSG group. LPO level and TAC in MSG intoxicated rats significantly normalized by NSS ingestion. NO level showed absence of significant difference among all experimental groups. MSG elicited histopathological lesions such as depleted glycogen content and fibrosis however, NSS succeeded in enhancing all these features. MSG group showed positive glutathione reductase and superoxide dismutase two immuno-expression whereas, MSG + NSS group showed weak immunostaining. A significant increase in the number of apoptotic cells was observed in MSG group compared to the control. On the other hand, MSG + NSS group exhibited a significant decrease in the number of apoptotic cells. NSS mitigated MSG-induced renal impairments by ameliorating oxidative stress and exerting anti-apoptotic effect.

Potential Ameliorative Effect of Crude Honeybee on Monosodium Glutamate Induced Nephrotoxicity to Male Rats

“…Rats were reared under the standard conditions of feeding, light/dark ratio (12:12 hrs), temperature (24±2 o C), and humidity (50±10%). The animals were allocated randomly into four groups (n= 6): the control group was administrated 1ml of saline daily orally, MSG group was administrated (30 gm/kg in diet), according to Abd-Elkareem et al [4] . The MSG was obtained from the Morgan Chemical industry, Egypt with a purity of 99%.…”

“…Glutamate, not sodium, is the component of MSG that negatively affects the body. Due to the kidney's abundance of glutamate receptors, MSG consumption makes the kidney a target for the glutamate [4] .…”

“…Elbassuoni et al [5] found that there were areas of renal interstitial hemorrhage and congested blood capillaries in rats treated with high-dose MSG. Longterm MSG administration has harmful properties on the internal organs such as renal upsetting redox homeostasis and encouraging histological lesions as well as lipid peroxidation [4] .…”

Effects of Apocynin against Monosodium Glutamate-Induced Oxidative Damage in Rat Kidne

“…The tissues were embedded in paraffin. Sections (at 5 µm) were then stained with hematoxylin-eosin (H&E) for general histopathologic assessment of renal tissue and with Periodic Acid-Schiff Reaction (PAS) to assess changes in the basement membrane and tubule brush border (14). The H&E-stained sections were semi-quantitatively scored for proximal and distal tubule damage, inflammatory cell infiltration, and vascular congestion (15).…”

Novel Umami-, Salty-, and Kokumi-Enhancing γ-Glutamyl Tripeptides Synthesized with the Bitter Dipeptides from Defatted Peanut Meal Protein Hydrolysate

“…1−4 Based on the rats model, consumption of pure monosodium glutamate (MSG) in an excessive overdose is associated with a wide range of adverse health effects, including nephrotoxicity, cardiotoxicity, hepatotoxicity, neurotoxicity, low-grade inflammation, metabolic disarray, and premalignant alterations. 5 In this context, it is commonly accepted and recommended to reduce the usage dose of sodium salt and MSG in foods. Researchers are looking for natural taste alternatives that simultaneously reduce sodium or MSG intake and maintain the sensory quality of food products.…”