Effect of Nicotinic Acid on Sperm Characteristic and Oocyte Development after <i>In Vitro</i> Fertilization using Cryopreserved Boar Semen

Kim, Yujin; Lee, Sang‐Hee; Lee, Yeon-Ju; Oh, Hae-In; Cheong, Hyeonsook; Yang, Boo‐Keun; Lee, Seunghyung; Park, Choon‐Keun

doi:10.12750/jet.2015.30.1.7

Cited by 5 publications

(7 citation statements)

References 34 publications

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“…All experiments regarding freezing extender preparation and semen cryopreservation were carried out according to previously study (Kim et al, ). Nicotinic acid (NA; Sigma, MO, USA) was dissolved in phosphate‐buffered saline (PBS) until 1.0 M which stock solution was used to dilute NA in freezing extender.…”

Section: Methodsmentioning

confidence: 99%

“…In a previous study, the NA not only improved sperm survival and the intact acrosome during liquid preservation but also improved viability and mitochondrial activity, inhibited the acrosome reaction and increased the fertilization ability of frozen–thawed boar sperm (Kim et al, ; Lee et al, ). In addition, the NA has stabilizing of free radical and ROS and cell membrane protection ability; however, influence of the NA on functions and PUFAs composition of plasma membrane during cryopreservation in boar sperm has not yet been clearly demonstrated.…”

Section: Introductionmentioning

confidence: 94%

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Effect of nicotinic acid on the plasma membrane function and polyunsaturated fatty acids composition during cryopreservation in boar sperm

Lee

Kim

Kang

et al. 2019

Reprod Domestic Animals

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This study was conducted to evaluate the effect of nicotinic acid on plasma membrane integrity and fatty acid composition in frozen–thawed boar sperm. Boar semen was cryopreserved using freezing extender containing nicotinic acid (NA), then plasma membrane integrity, osmotic equilibration, lipid peroxidation and fatty acid were analysed. The plasma membrane integrity of frozen–thawed sperm was significantly higher in the 10 mM NA than in the 0 and 20 mM NA treatment groups (p < 0.05). Additionally, the osmotic equilibration ability was not different in treatment groups, but lipid peroxidation was significantly decreased in the 10 mM NA treatment group (p < 0.05). The saturated fatty acids were significantly decreased in the 10 mM NA treatment group, and C18:1n‐9, C18:2n‐6, C20:4n‐6, C22:5n‐6 and C22:6n‐3, and total polyunsaturated fatty acids (PUFAs) were significantly increased in the 10 mM NA treatment groups (p < 0.05). In summary, 10 mM NA improved plasma membrane integrity, inhibited lipid peroxidation and increased PUFAs in frozen–thawed boar sperm. These results suggest that NA may be useful to protect the plasma membrane and inhibit the loss of PUFAs for sperm cryopreservation in pigs.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

Effect of nicotinic acid on the plasma membrane function and polyunsaturated fatty acids composition during cryopreservation in boar sperm

Lee

Kim

Kang

et al. 2019

Reprod Domestic Animals

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“…A previous study carried a freezing extender [13,14]. First, the semen samples were centrifuged to remove seminal plasma for removing seminal plasma at 400 g for 5 min, and then, sperm fraction was only used for cryopreservation.…”

Section: Freezing and Thawing Of Semenmentioning

confidence: 99%

“…Sperm were diluted with 1 st freezing extender at 18℃ and was cooling at 5℃ for 120 min, then diluted with 2 nd freezing extender at 5℃ of the half volume of 1 st freezing extender until 1.0 × 10 9 sperm/mL. Sperm was packaged into 0.5 mL straw (20 straws/semen/pig) and cooled to -120℃ for 10 min before being plunged into liquid nitrogen for storage using static nitrogen vapor [12,13]. The frozen sperm into straw was thawed in a water bath at 37.0℃ for 45 seconds and centrifuged at 410 g for 5 min, and then the freezing extender was removed.…”

Section: Freezing and Thawing Of Semenmentioning

confidence: 99%

Effects of Melatonin and Silymarin on Reactive Oxygen Species, Nitric Oxide Production, and Sperm Viability and Motility during Sperm Freezing in Pigs

Lee¹,

Lee²

2023

Preprint

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Sperm during the freezing and thawing process is damaged by oxidative stress. Thus, its antioxidant scavenger is essential for sperm survival and death in frozen-thawed semen. We used melatonin and silymarin in experiments. Our study aimed to identify the effect of melatonin and silymarin on the motility and viability of sperm, reactive oxygen species (ROS), and nitric oxide (NO) production in frozen-thawed boar semen. Melatonin and silymarin were treated alone and co-treated in the fresh boar semen. Boar semen was collected using the gloved-hand method from ten crossbred pigs, and the age of the experimental ten male pigs was 28.7 ± 3.2 months. We evaluated sperm viability using SYBR-14 and PI kit, and ROS and NO production were detected by DCF-DA and DAF-2, respectively. The sperm motility was not significantly different between non-treatment and treatment. ROS and NO production in frozen-thawed sperm were decreased by melatonin and silymarin (P < 0.05). Moreover, silymarin significantly reduced NO production more than melatonin (P < 0.05). Melatonin (46.37 ± 1.10) and silymarin (46.13 ± 0.80) enhanced the viability of sperm (P < 0.05). We suggest that melatonin and silymarin are essential antioxidants in semen cryopreservation for protecting sperm damage and maintaining sperm viability. Melatonin and silymarin may be useful antioxidants in freezing boar sperm.

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“…First, the semen samples were centrifuged to remove seminal plasma for removing seminal plasma at 400× g for 5 min, and then, sperm fraction was only used for cryopreser-vation [30,31]. Briefly, the first freezing extender was composed of 11.0% α-lactose (Sigma, St. Louis, MO, USA) and 20.0% egg yolk, and second freezing extender was made from the first freezing extender supplemented with 9.0% glycerol (Sigma) and 1.5% OEP (Nova Chemical Sales Inc., Scituate, MA, USA).…”

Section: Freezing and Thawing Of Semenmentioning

confidence: 99%

Effects of Melatonin and Silymarin on Reactive Oxygen Species, Nitric Oxide Production, and Sperm Viability and Motility during Sperm Freezing in Pigs

Lee

2023

Animals

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Sperm during the freezing and thawing process is damaged by oxidative stress. Thus, its antioxidant scavenger is essential for sperm survival and death in frozen–thawed semen. We used melatonin and silymarin in experiments after the dose-dependent experiment. Our study aimed to identify the effect of melatonin and silymarin on the motility and viability of sperm, reactive oxygen species (ROS), and nitric oxide (NO) production in frozen–thawed boar semen. Melatonin and silymarin were treated alone and cotreated in the fresh boar semen. Boar semen was collected using the gloved-hand method from ten crossbred pigs, and samples were used in the experiments. We evaluated sperm viability using SYBR-14 and PI kit, and ROS and NO production were detected by DCF-DA and DAF-2, respectively. The sperm motility was not significantly different between non-treatment and treatment. ROS and NO production in frozen–thawed sperm were decreased by melatonin and silymarin. Moreover, silymarin significantly reduced NO production more than melatonin. Melatonin and silymarin enhanced the viability of sperm. We suggest that melatonin and silymarin are essential antioxidants in semen cryopreservation for protecting sperm damage and maintaining sperm viability. Melatonin and silymarin may be useful antioxidants in freezing boar sperm.

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Effect of Nicotinic Acid on Sperm Characteristic and Oocyte Development after In Vitro Fertilization using Cryopreserved Boar Semen

Cited by 5 publications

References 34 publications

Effect of nicotinic acid on the plasma membrane function and polyunsaturated fatty acids composition during cryopreservation in boar sperm

Effect of nicotinic acid on the plasma membrane function and polyunsaturated fatty acids composition during cryopreservation in boar sperm

Effects of Melatonin and Silymarin on Reactive Oxygen Species, Nitric Oxide Production, and Sperm Viability and Motility during Sperm Freezing in Pigs

Effects of Melatonin and Silymarin on Reactive Oxygen Species, Nitric Oxide Production, and Sperm Viability and Motility during Sperm Freezing in Pigs

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