Effect of nicotinic acid on the plasma membrane function and polyunsaturated fatty acids composition during cryopreservation in boar sperm

Lee, Sang-Hee; Kim, Yujin; Kang, Byeong Ho; Park, Choon‐Keun

doi:10.1111/rda.13508

Cited by 9 publications

(4 citation statements)

References 26 publications

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“…When ROS production exceeds goat sperm’s ROS scavenging ability, sperm are subjected to oxidative stress and their functioning deteriorates [ 42 ]. It is known that sperm membrane contains a high amount of polyunsaturated fatty acids (PUFA) [ 43 ]. The structure of PUFA is susceptible to ROS induced damage and the initiation of LPO leads to a change in sperm membrane composition and biophysical properties [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Beneficial Effect of Proline Supplementation on Goat Spermatozoa Quality during Cryopreservation

Zhang

Min

Lang

et al. 2022

Animals

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Sperm cryopreservation contributes to the extensive utilization of artificial insemination (AI) in the daily livestock industry. However, due to the presence of few sperm with good biological function in post-thaw goat sperm, its use has been limited for AI purposes. Hence, its improvement has been the focus of many research studies. This study aimed to investigate the effects of proline supplementation of the freezing medium on goat sperm. The goat semen was cryopreserved with freezing medium supplementation of different concentrations of proline (0, 0.5, 1, 2 and 4 mM). The post-thaw sperm motility patterns, membrane integrity, acrosome integrity, lipid peroxidation (LPO) levels, malondialdehyde (MDA) levels, total antioxidant capacity (T-AOC), proline dehydrogenase (PRODH) activity, superoxide dis-mutase (SOD) activity, glutathione (GSH) levels and GSH/GSSG were evaluated. Likewise, the expression and immunofluorescent localization of PRODH in post-thaw goat sperm was also detected. It was observed that addition of 2 mM proline to the freezing medium significantly enhanced post-thaw goat sperm total motility, progressive motility, straight-linear velocity (VSL), curvilinear velocity (VCL), average path velocity (VAP), straightness (STR), linearity (LIN), membrane integrity and acrosome integrity. Interestingly, PRODH was expressed in post-thaw goat sperm, especially in the post-acrosome and sperm tail. Addition of 2 mM proline also significantly increased the post-thaw sperm PRODH activity compared to the control. Moreover, post-thaw goat sperm LPO levels and MDA levels were reduced by supplementation of 2 mM proline. Furthermore, compared to the control, the values of post-thaw goat sperm T-AOC, SOD activity, GSH level and GSH/GSSG were also significantly increased in 2 mM proline treatment. Reduction of post-thaw goat sperm apoptosis in 2 mM proline treatment was also observed as the levels of Caspase3 and Caspase9 were decreased by the supplementation with 2 mM proline. These observations suggest that the addition of 2 mM proline to the freezing medium increased post-thaw goat sperm quality by reducing oxidative stress during cryopreservation. These findings also provide novel insights into the use of proline as an efficient additive to enhance post-thaw goat sperm quality during cryopreservation.

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Section: Discussionmentioning

confidence: 99%

Beneficial Effect of Proline Supplementation on Goat Spermatozoa Quality during Cryopreservation

Zhang

Min

Lang

et al. 2022

Animals

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“…In this study, camellia oil with its high constituent of omega 6, 9, palmitic acid and stearic acid also revealed its antioxidant capacity by protecting sperm from cryodamage associated with sperm freezing and thawing. However, too high a concentration of camellia oil showed a slightly cytotoxic effect on sperm qualities, which have also been reported in other antioxidants [ 45 , 46 ]. The reason might be that an excess of antioxidants may disturb the balance between free radicals and antioxidants in mammalian cells [ 47 ].…”

Section: Discussionmentioning

confidence: 58%

Camellia oil with its rich in fatty acids enhances post-thawed boar sperm quality

Khophloiklang,

Chanapiwat,

Kaeoket

2024

Acta Vet Scand

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Background Boar sperm are highly susceptible to specific conditions during cryopreservation, leading to a significant decrease in their fertilizing potential due to damage to their membranes. Camellia oil, known for its fatty acids with antioxidant and biological properties, has not been previously explored for the cryopreservation of boar semen. This study aimed to examine the effects of camellia oil on post-thawed boar sperm quality. Boar semen ejaculates (n = 9) were collected and divided into six equal aliquots based on camellia oil concentrations (0, 0.5, 1, 1.5, 2 and 2.5% v/v) in the freezing extender. Semen samples were processed and cryopreserved using the liquid nitrogen vapor method. Thereafter, frozen semen samples were thawed at 50 °C for 12 s and evaluated for sperm morphology by scanning electron microscope, sperm motility using a computer-assisted sperm analyzer, sperm viability, acrosome integrity, mitochondrial function, MDA level and total antioxidant capacity. Results The results demonstrated that the supplementation of 1.5% (v/v) camellia oil showed superior post-thaw sperm qualities such as improved sperm morphology, motility, acrosome integrity and mitochondrial function by 14.3%, 14.3% and 11.7%, respectively, when compared to the control group. Camellia oil at a concentration of 1.5% (v/v) showed the lowest level of MDA (18.3 ± 2.1 µmol/L) compared to the other groups. Conclusions In conclusion, adding 1.5% (v/v) camellia oil in the freezing extender reduced the oxidative damage associated with cryopreservation and resulted in a higher post-thawed sperm quality.

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“…The boar sperm is more sensitive to lipid peroxidation than other species because the polyunsaturated fatty acids (PUFAs) content of the plasma membrane is higher than that of other species. consequentially, they are particularly vulnerable to attack by free radicals and ROS and are thus more susceptible to lipid peroxidation (Lee et al, 2019). In this sense, the accumulation of reactive oxygen species (ROS) can cause gradual oxidative damage, affect cellular function, and cause alterations in DNA, proteins and lipids (Gulcin, 2020).…”

Section: Introductionmentioning

confidence: 99%

Low‐density lipoproteins, resveratrol and quercetin as alternative additives to improve boar semen cooling

González,

Rojas,

Rojano

et al. 2023

Reprod Domestic Animals

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Low‐density lipoproteins (LDL), quercetin (Q) and resveratrol (R) have been used for sperm preservation to improve sperm quality in different species. To evaluate the effects of LDL, Q and R during the cooling of boar semen. Fifteen boar semen samples were diluted in a BTS extender supplemented with the treatments: LDL at 6%, Q at 10 μM (Q10), 30 μM (Q30) and 50 μM (Q50), or R at 10 μM (R10), 30 μM (R30) and 50 μM. A control without supplementation was included. The semen was stored by cooling at 16°C for 96 h. Every 24 h, sperm motility and kinetics were evaluated using a computer‐assisted sperm analyzer (IVOS). At 24 and 96 h of cooling, functional membrane integrity and mitochondrial membrane potential (ΔΨM) of sperm were evaluated by the hypoosmotic swelling test (HOST) an flow cytometry with JC‐1 probe, respectively, LDL improved progressive motility of sperm during cooling. Likewise, LDL increased average path velocity (VAP) and straight‐line velocity (VSL) and/or curvilinear velocity (VCL) during the first 48 h of cooling. The use of Q between 10 and 30 μM caused a reduction in total motility, progressive motility and amplitude of the lateral head displacement during the entire cooling period, as well as a decrease in VAP, VSL and VCL at 96 h of cooling. LDL, Q10, Q30 and Q50 modulated mitochondrial activity by reducing high‐ΔΨM sperm at 0 and 96 h of cooling. During the cooling of the boar semen prior to artificial insemination, the parameters of sperm quality that could influence fertility decrease; however, the inclusion of antioxidants and additives that protect the plasma membrane, such as LDL, could mitigate the damaging effects on spermatozoa. It is concluded that LDL can improve the motility and kinetics of boar semen during cooling while it could modulating the sperm's mitochondrial activity. On the contrary, Q could alter the motility and kinetics of boar sperm during the cooling period.

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Effect of nicotinic acid on the plasma membrane function and polyunsaturated fatty acids composition during cryopreservation in boar sperm

Cited by 9 publications

References 26 publications

Beneficial Effect of Proline Supplementation on Goat Spermatozoa Quality during Cryopreservation

Beneficial Effect of Proline Supplementation on Goat Spermatozoa Quality during Cryopreservation

Camellia oil with its rich in fatty acids enhances post-thawed boar sperm quality

Low‐density lipoproteins, resveratrol and quercetin as alternative additives to improve boar semen cooling

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