Abstract:The effect of natural selection on microsatellite simple sequence repeat (SSR) alleles was investigated in two distinct common bean (Phaseolus vulgaris) generations (F 8 and F 24 ) derived from the cross between the P. vulgaris cultivars Carioca MG x ESAL 686. The F 2 segregant population was propagated by the bulk method and 107 plants were sampled in two generations (F 8 and F 24 ). Each plant generated one family which was replicated by the bulk method to F 8:11 and F 24:27 families from which DNA was extr… Show more
“…Nevertheless, this did not occur, probably due to the occurrence of a varietal mixture or cross pollination in the experimental stations of the institutions supplying the cultivars. It is necessary to consider, however, that in various SSR loci in common bean, there is heterozygosis even after 24 generations of self-pollination (Rodrigues and dos Santos, 2006).…”
Section: Resultsmentioning
confidence: 99%
“…Additionally, the kinship observed may also be due, in part, to the cultivars having loci in heterozygosis (H O ) in an amount greater than expected for autogamous plants, at least partially reflecting the occurrence of a mixture and the heterozygosis maintained through natural selection in autogamous plants like common bean (Rodrigues and dos Santos, 2006) (Table 4). This may also be observed in the dendrogram (Figure 2).…”
“…Nevertheless, this did not occur, probably due to the occurrence of a varietal mixture or cross pollination in the experimental stations of the institutions supplying the cultivars. It is necessary to consider, however, that in various SSR loci in common bean, there is heterozygosis even after 24 generations of self-pollination (Rodrigues and dos Santos, 2006).…”
Section: Resultsmentioning
confidence: 99%
“…Additionally, the kinship observed may also be due, in part, to the cultivars having loci in heterozygosis (H O ) in an amount greater than expected for autogamous plants, at least partially reflecting the occurrence of a mixture and the heterozygosis maintained through natural selection in autogamous plants like common bean (Rodrigues and dos Santos, 2006) (Table 4). This may also be observed in the dendrogram (Figure 2).…”
“…DNA was extracted from the parents, CNFC 9506 and RP-2, and from the 186 F 2 progenies according to the procedures described by Rodrigues and Santos (2006). Nucleic acids were rehydrated in TE buffer and quantified by 1% agarose gel electrophoresis using DNA markers of known concentrations.…”
ABSTRACT. In this study, we identified simple sequence repeat, amplified fragment length polymorphism, and sequence-related amplified polymorphism markers linked to quantitative trait loci (QTLs) for resistance to white mold disease in common bean progenies derived from a cross between lines CNFC 9506 and RP-2, evaluated using the oxalic acid test and using Bayesian analysis. DNA was extracted from 186 F 2 plants and their parental lines for molecular analysis. Fifteen experiments were carried out for phenotypic analysis, which included 186 F 2:4 progenies, the F 1 generation, the F 2 generation, and the lines CNFC 9506, RP-2, and G122 as common treatments. A completely randomized experimental design with 3 replications was used in controlled environments. The adjusted means for the F 2:4 generation were to identify QTLs by Bayesian shrinkage analysis. Significant differences were observed among the progenies for the reaction to white mold. The moving away method under the Bayes- Identification of QTLs for resistance to white mold ian approach was effective for identifying QTLs when it was not possible to obtain a genetic map because of low marker density. Using the Wald test, 25 markers identified QTLs for resistance to white mold, as well as 16 simple sequence repeats, 7 amplified fragment length polymorphisms, and 2 sequence-related amplified polymorphisms. The markers BM184, BM211, and PV-gaat001 showed low distances from QTLs related white mold resistance. In addition, these markers showed, signal effects with increasing resistance to white mold and high heritability in the analysis with oxalic acid, and thus, are promising for marker-assisted selection.
“…Microsatellites have been used for plant genetic analysis such as to measure the effects of natural selection (Rodrigues and Santos 2006), to unveil the genetic diversity (Vieira et al 2009, Guan et al 2010, to measure population structure (Ribeiro et al 2010, Albertini et al 2011, to integrate the genetic, physical and sequence-based physical maps (McClean et al 2010) and for marker assisted selection (Benchimol et al 2005, Chen et al 2011). …”
Section: Application Of Microsatellites In Plant Breedingmentioning
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.