Effect of Nanosecond Pulsed Currents on Directions of Cell Elongation and Migration through Time-Lapse Analysis

Tada, Hayato; Uehara, Satoshi; Chang, Chia-Hsing; Yano, Ken’ichi; Sato, Takehiko

doi:10.3390/ijms24043826

Cited by 4 publications

(4 citation statements)

References 31 publications

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“…Considering that all three conditions (ELE, POS, and NEG) receive the same amount of electricity, it is interesting to see the differences between the electrical effects and the chemical effects. Regarding cell motility, previous our study has shown that the nanosecond pulsed current without plasma discharge do not result in a constant change in the direction of cell elongation or motility [32]. Based on the similar response to nanosecond pulsed currents with and without plasma discharges, motility with plasma discharges probably shows the same trend as without plasma discharges.…”

Section: Electrical Effects Of Plasmasupporting

confidence: 55%

Cellular response to plasma-generated electrical and chemical stimulation of equal electricity

Nakayama,

Liu,

Yano

et al. 2024

J. Phys. D: Appl. Phys.

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Plasma medicine is a novel field of research that has the potential in life sciences, including cancer treatment. It has been believed that all of these potential applications depend on the chemical factor of the plasma. However, the electrical factors that are produced simultaneously with the chemical factors have not been researched thoroughly. To assess the effect of plasma on the response of cells, a system that enables simultaneous comparison of the effects of chemical and electrical factors of plasma with equal electricity was invented.This system separates the polarity of plasma and isolates the chemical species from the electric stimulation. Using this system, HT-1080 cells were exposed to plasma for 10 minutes, 1 hour, and 24 hours. The 10-minute plasma treatment showed a clear difference in the polarity of plasma, where cells under exposure to positive plasma died while cells in other conditions survived. An hour of plasma treatment affected the cells under the negative plasma, where the cell viability was reduced to half. Meanwhile, the electric stimulation did not affect the cell viability but did alter the cell membrane. Collectively, this study demonstrates the differential effects of three factors (electric factor, positive plasma, negative plasma) under the same condition: both the electrical and chemical effects of plasma-generated stimuli with equal amounts of electricity were successfully observed.

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Section: Electrical Effects Of Plasmasupporting

confidence: 55%

Cellular response to plasma-generated electrical and chemical stimulation of equal electricity

Nakayama,

Liu,

Yano

et al. 2024

J. Phys. D: Appl. Phys.

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“…Generally human or animal cells elongate perpendicularly to the electric field and move in the direction of the electric field. Recently, Tada et al, showed that even plasmabased nanosecond pulsed current elongates cells, however, the direction of the movement and elongation was not defined [2]. In this research article, they used observed cell physiological behaviors after applying plasma stimulated pulse current.…”

Section: Plasma-induced Cell Motility and Wound Healingmentioning

confidence: 98%

Plasma Bioscience and Medicine Molecular Research

Kaushik

Choi

2023

IJMS

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This special issue delivers an applied and basic platform for exchanging advanced approaches or research performance that link the plasma physics research in cell biology, cancer treatments, immunomodulation, stem cell differentiation, nanomaterial synthesis, and their applications, agriculture and food processing, microbial inactivation, water decontamination, and sterilization applications, including in vitro and in vivo research [...]

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“…Recently, Chang et al . and his colleagues constructed the system to evaluate the effect of plasma-induced nanosecond pulsed current and chemical factors on human HT-1080 cells activities and observed a significant increase in cell migration along with altered cell morphology 19 – 22 . Mendis et al .…”

Section: Introductionmentioning

confidence: 99%

Influence of electric potential-induced by atmospheric pressure plasma on cell response

Okumura,

Chang,

Koga

et al. 2023

Sci Rep

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Plasma irradiation leads not only active species, but also reactive chemical species, ultraviolet light, electric fields, magnetic fields, and shock waves. To date the effects of reactive chemical species have been mainly discussed. To understand the biological effect caused by an electric potential induced with an atmospheric-pressure plasma, the behavior of cell stimulated by electric potential was investigated using HeLa cell. The cell concentration assay revealed that less than 20% of cells inactivated by potential stimulation and the remained cells proliferate afterward. Fluorescent microscopic observation revealed that potential stimulation is appreciable to transport the molecules through membrane. These results show that potential stimulation induces intracellular and extracellular molecular transport, while the stimulation has a low lethal effect. A possible mechanism for this molecular transport by potential stimulation was also shown using numerical simulation based on an equivalent circuit of the experimental system including adhered HeLa cell. The potential formation caused by plasma generation is decisive in the contribution of plasma science to molecular biology and the elucidation of the mechanism underlying a biological response induction by plasma irradiation.

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Effect of Nanosecond Pulsed Currents on Directions of Cell Elongation and Migration through Time-Lapse Analysis

Cited by 4 publications

References 31 publications

Cellular response to plasma-generated electrical and chemical stimulation of equal electricity

Cellular response to plasma-generated electrical and chemical stimulation of equal electricity

Plasma Bioscience and Medicine Molecular Research

Influence of electric potential-induced by atmospheric pressure plasma on cell response

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