Influence of electric potential-induced by atmospheric pressure plasma on cell response

Abstract

Plasma irradiation leads not only active species, but also reactive chemical species, ultraviolet light, electric fields, magnetic fields, and shock waves. To date the effects of reactive chemical species have been mainly discussed. To understand the biological effect caused by an electric potential induced with an atmospheric-pressure plasma, the behavior of cell stimulated by electric potential was investigated using HeLa cell. The cell concentration assay revealed that less than 20% of cells inactivated by potential stimulation and the remained cells proliferate afterward. Fluorescent microscopic observation revealed that potential stimulation is appreciable to transport the molecules through membrane. These results show that potential stimulation induces intracellular and extracellular molecular transport, while the stimulation has a low lethal effect. A possible mechanism for this molecular transport by potential stimulation was also shown using numerical simulation based on an equivalent circuit of the experimental system including adhered HeLa cell. The potential formation caused by plasma generation is decisive in the contribution of plasma science to molecular biology and the elucidation of the mechanism underlying a biological response induction by plasma irradiation.

Plasma–liquid interactions in the presence of organic matter—A perspective

“… 103 Recently published results indicate that the application of a discharge generates electric potential in the solution covering a cell culture. 104,105 Cells exposed to this potential stimulation without any exposure to plasma-generated species showed an increased membrane permeability to large molecules (temporary electroporation) with low lethal effects. This suggests that the plasma-induced potential in the liquid may enhance the action of plasma-generated RONS.…”