Effect of NaF on cAMP Accumulation, cAMP-dependent Protein Kinase Activity in, and Amylase Secretion from, Rat Parotid Gland Cells

Shahed, Asha; Allmann, David W.

doi:10.1177/00220345880670020501

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…IP injections of NaF (15 mg F − /kg b.w) increased cAMP concentration in SM gland of rats after 30 and 60 min [55]. The same increased level of cAMP was observed for PA gland cells of rats incubated 0.01 mmol/L of NaF after 10 min [56]. Low concentrations stimulate LPO, and at high and very high concentrations may act as inhibitor of MDA generation [47].…”

Section: Discussionmentioning

confidence: 88%

Effects of Single Exposure of Sodium Fluoride on Lipid Peroxidation and Antioxidant Enzymes in Salivary Glands of Rats

Yamaguti

Simões

Ganzerla

et al. 2013

Oxidative Medicine and Cellular Longevity

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Many studies suggest that fluoride exposure can inhibit the activity of various enzymes and can generate free radicals, which interfere with antioxidant defence mechanisms in living systems. To further the understanding of this issue, this present study examined the effects of low-dose fluoride treatment on the activity of enzymatic antioxidant superoxide dismutase (SOD) and catalase (CAT), as well as the levels of lipid peroxidation (LPO) in the parotid (PA) and submandibular (SM) salivary glands of rats. Rats were injected with a single dose of sodium fluoride (NaF) (15 mg F−/kg b.w.) then euthanized at various time intervals up to 24 hours (h) following exposure. NaF exposure did not cause significant differences in SOD or CAT activity or LPO levels in PA glands compared to control. Conversely, SM glands presented increased SOD activity after 3 h and decreased SOD activity after 1, 12, and 24 h, while LPO was increased after 6, 12, and 24 h of the NaF injection. There were no significant differences in the CAT activity in the groups studied. Our results demonstrated that NaF intoxication caused oxidative stress in salivary glands few hours after administration. These changes were more pronounced in SM than in PA gland.

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Section: Discussionmentioning

confidence: 88%

Effects of Single Exposure of Sodium Fluoride on Lipid Peroxidation and Antioxidant Enzymes in Salivary Glands of Rats

Yamaguti

Simões

Ganzerla

et al. 2013

Oxidative Medicine and Cellular Longevity

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show abstract

“…Figura 2.3 -Curva de concentração plasmática do fluoreto após ingestão do íon. O pico ocorre normalmente após 1 hora e depende de diversos fatores como a absorção no trato gastro-intestinal, distribuição para o plasma, excreção através da urina e captação pelos tecidos mineralizados (WHITFORD, 1990) SRINIVASALU; SUBRAMANIAN, 2004;SINGH;KANWAR, 1981;SONI;KACHOLE;PAWAR, 1984), intestino (SHAYIQ; RAZA; KIDWAI, 1986;SONI;KACHOLE;PAWAR, 1984), pulmões (SONI;KACHOLE;PAWAR, 1984), glândulas salivares KLEINER, 1980;DA MOTTA;DE SOUZA;NICOLAU, 1999;NICOLAU;RIBEIRO, 1992;ALLMANN, 1988) BURT, 1996;WHITFORD, 1989).…”

Section: Introductionunclassified

Estudo da administração de flúor e o estresse oxidativo em glândulas salivares de ratos

Yamaguti¹

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Fluoride (F) is a toxicological axiom. Many reports have proposed that in varying concentrations, F induces increased reactive oxygen species generation, enhanced lipid peroxidation and impairs the antioxidant enzyme defence system in blood and tissues of experimental animals causing several biochemical alterations. Although the most pronounced effects of F intake are manifested in bones and teeth, soft tissues are also affected. Due to the paucity of reports investigating the short-and long-term effects of varied doses of F on soft tissues, especially salivary glands, this study aimed to evaluate the acute and long-term effects of sodium fluoride (NaF) exposure on antioxidant enzyme defence system and lipid peroxidation in the submandibular (SM) and parotid (PA) salivary glands of rats. For the acute investigation, the experimental groups of rats were injected intraperitoneally with NaF solution (15 mg F-/kg b.w.), while control groups were administered the same volume of sodium chloride solution (0,9%). The animals were euthanized in groups 1, 3, 6, 12 and 24 hours after injection. To evaluate long-term exposure effects, experimental groups of rats were administered 100 pmm Fin their drinking water for 30, 60 and 90 days. The control groups were provided with untreated tap water over the same periods. In all groups, after euthanization the SM and PA glands of each rat were extracted and analyzed for superoxide dismutase (SOD) and catalase (CAT) activities, malondialdehyde (MDA), protein and blood glycemia content. For both acute and long-term experiments, the experimental groups demonstrated higher levels of glycemia, altered levels of SOD and CAT in both glands, and increased levels of MDA in blood and salivary tissues of both glands. There were no differences in protein content for the acute experiment, but in the long-term experiment, increased protein levels were observed after 90 days in the SM gland and decreased protein levels were observed in the PA gland after 30 and 60 days. These results suggest that F impaired the antioxidant defence system and enhanced the levels of MDA in blood and SM and PA salivary glands of rats.

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GTP-binding proteins in cell survival and demise: the emerging picture in the pancreatic β-cell

Kowluru

Morgan

2002

Biochemical Pharmacology

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Effect of NaF on cAMP Accumulation, cAMP-dependent Protein Kinase Activity in, and Amylase Secretion from, Rat Parotid Gland Cells

Cited by 4 publications

References 25 publications

Effects of Single Exposure of Sodium Fluoride on Lipid Peroxidation and Antioxidant Enzymes in Salivary Glands of Rats

Effects of Single Exposure of Sodium Fluoride on Lipid Peroxidation and Antioxidant Enzymes in Salivary Glands of Rats

Estudo da administração de flúor e o estresse oxidativo em glândulas salivares de ratos

GTP-binding proteins in cell survival and demise: the emerging picture in the pancreatic β-cell

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