Effect of Myomectomy on Endometrial Glutathione Peroxidase 3 (GPx3) and Glycodelin mRNA Expression at the Time of the Implantation Window

Sanoee, Marzieh Farimani; Alizamir, Tahereh; Faramarzi, Shamila; Saidijam, Massoud; Yadegarazari, Reza; Shabab, Nooshin; Haghi, Alireza Rastgoo; Alizadeh, Zohreh

doi:10.6091/ibj.1222.2013

Cited by 5 publications

(1 citation statement)

References 32 publications

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“…GPX3 plays a major role in reducing ROS during decidualization with its expression peaks during the implantation window. While inhibition of GPX3 is associated with a reduced pregnancy rate (27)(28)(29). TAGLN expression is significantly upregulated in the receptive endometrium compared to the pre-receptive endometrium (28) and is involved in regulating cell invasion, migration, and differentiation (30).…”

Section: Discussionmentioning

confidence: 99%

Integrated Analysis of Multiple Microarrays Based on Raw Data Identified Novel Gene Signatures in Recurrent Implantation Failure

Zeng

Shen

et al. 2022

Front. Endocrinol.

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BackgroundRecurrent implantation failure (RIF) is an intricate complication following IVF-ET, which refers to the situation that good-quality embryos repeatedly fail to implant following two or more IVF cycles. Intrinsic molecular mechanisms underlying RIF have not yet been fully elucidated. With enormous improvement in high-throughput technologies, researchers screened biomarkers for RIF using microarray. However, the findings of published studies are inconsistent. An integrated study on the endometrial molecular determinants of implantation will help to improve pregnancy outcomes.ObjectiveTo identify robust differentially expressed genes (DEGs) and hub genes in endometrium associated with RIF, and to investigate the diagnostic role of hub genes in RIF.MethodsRaw data from five GEO microarrays regarding RIF were analyzed. Integrated genetic expression analyses were performed using the Robust Rank Aggregation method to identify robust DEGs. Enrichment analysis and protein-protein interaction (PPI) analysis were further performed with the robust DEGs. Cytohubba was used to screen hub genes based on the PPI network. GSE111974 was used to validate the expression and diagnostic role of hub genes in RIF.Results1532 Robust DEGs were identified by integrating four GEO datasets. Enrichment analysis showed that the robust DEGs were mainly enriched in processes associated with extracellular matrix remodeling, adhesion, coagulation, and immunity. A total of 18 hub genes (HMGCS1, SQLE, ESR1, LAMC1, HOXB4, PIP5K1B, GNG11, GPX3, PAX2, TF, ALDH6A1, IDH1, SALL1, EYA1, TAGLN, TPD52L1, ST6GALNAC1, NNMT) were identified. 10 of the 18 hub genes were significantly differentially expressed in RIF patients as validated by GSE111974. The 10 hub genes (SQLE, LAMC1, HOXB4, PIP5K1B, PAX2, ALDH6A1, SALL1, EYA1, TAGLN, ST6GALNAC1) were effective in predicting RIF with an accuracy rate of 85%, specificity rate of 100%, and sensitivity rate of 88.9%.ConclusionsOur integrated analysis identified novel robust DEGs and hub genes in RIF. The hub genes were effective in predicting RIF and will contribute to the understanding of comprehensive molecular mechanisms in RIF pathogenesis.

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Section: Discussionmentioning

confidence: 99%

Integrated Analysis of Multiple Microarrays Based on Raw Data Identified Novel Gene Signatures in Recurrent Implantation Failure

Zeng

Shen

et al. 2022

Front. Endocrinol.

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Diagnostic Office Vaginohysteroscopy in Evaluation of Infertility Prior to IVF: A Retrospective Analysis of 1000 Cases

Kumar

Mohan

Talwar

et al. 2017

J Obstet Gynecol India

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Potential causes of subfertility in patients with intramural fibroids

Pier

Bates

2015

Fertil Res and Pract

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BackgroundIntramural leiomyomas have been long debated as a potential cause of infertility and pregnancy loss.FindingsPrevious research has linked intramural fibroids to defective implantation, as well as to abnormal peristaltic events of the uterine smooth muscle. Previous reports describe the effects of intramural fibroids on normal human fertility and early pregnancy loss, specifically in regards to implantation failure.Conclusion A thorough understanding of prior research may direct new research focus, leading to better understanding of leiomyoma-associated infertility.

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Effect of Myomectomy on Endometrial Glutathione Peroxidase 3 (GPx3) and Glycodelin mRNA Expression at the Time of the Implantation Window

Cited by 5 publications

References 32 publications

Integrated Analysis of Multiple Microarrays Based on Raw Data Identified Novel Gene Signatures in Recurrent Implantation Failure

Integrated Analysis of Multiple Microarrays Based on Raw Data Identified Novel Gene Signatures in Recurrent Implantation Failure

Diagnostic Office Vaginohysteroscopy in Evaluation of Infertility Prior to IVF: A Retrospective Analysis of 1000 Cases

Potential causes of subfertility in patients with intramural fibroids

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