Effect of mycophenolic acid on proliferation of dermal papilla cells and induction of anagen hair follicles

Hair disorders such as hair loss (alopecia) and androgen dependent, excessive hair growth (hirsutism, hypertrichosis) may impact the social and psychological well-being of an individual. Recent advances in understanding the biology of hair have accelerated the research and development of novel therapeutic and cosmetic hair growth agents. Preclinical models aid in dermocosmetic efficacy testing and claim substantiation of hair growth modulators. The in vitro models to investigate hair growth utilize the hair follicle Dermal Papilla cells (DPCs), specialized mesenchymal cells located at the base of hair follicle that play essential roles in hair follicular morphogenesis and postnatal hair growth cycles. In this review, we have compiled and discussed the extensively reported literature citing DPCs as in vitro model to study hair growth promoting and inhibitory effects. A variety of agents such as herbal and natural extracts, growth factors and cytokines, platelet-rich plasma, placental extract, stem cells and conditioned medium, peptides, hormones, lipid-nanocarrier, light, electrical and electromagnetic field stimulation, androgens and their analogs, stress-serum and chemotherapeutic agents etc. have been examined for their hair growth modulating effects in DPCs. Effects on DPCs' activity were determined from untreated (basal) or stress induced levels. Cell proliferation, apoptosis and secretion of growth factors were included as primary end-point markers. Effects on a wide range of biomolecules and mechanistic pathways that play key role in the biology of hair growth were also investigated. This consolidated and comprehensive review summarizes the up-to-date information and understanding regarding DPCs based screening models for hair growth and may be helpful for researchers to select the appropriate assay system and biomarkers. This review highlights the pivotal role of DPCs in the forefront of hair research as screening platforms by providing insights into mechanistic action at cellular level, which may further direct the development of novel hair growth modulators.

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“…In addition, various other compounds have demonstrated stimulatory effect on DPCs at molecular level .…”

Section: Hair Growth Promoting Effectmentioning

confidence: 99%

Review of Hair Follicle Dermal Papilla cells asin vitroscreening model for hair growth

Madaan

Verma

Singh

et al. 2018

Intern J of Cosmetic Sci

113

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“…In another animal study assessing the hair growth effect of mycophenolic acid (MP), microneedling was found to accelerate the stimulatory growth of topical MP on anagen follicles [48].…”

Section: Evidence For Efficacymentioning

confidence: 99%

Cosmetic Procedures in the Treatment of Alopecia

Kartal¹,

Altunel²,

Gençler³

2017

Hair and Scalp Disorders

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Alopecia has a significant negative impact on the quality of life. Unfortunately, there is no satisfactory cure for most types of alopecia. Alopecia is divided into cicatricial and noncicatricial types. Androgenetic alopecia, alopecia areata, and telogen effluvium are common forms of noncicatricial alopecias. In order to treat or improve the appearance, various procedures that are being applied for different types of alopecia including mesotherapy, microneedling, platelet-rich plasma, low-level light therapy, and stem-cell therapy with variable outcomes are reviewed in this chapter.

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“…The protocol for western blot analysis was described in a previous report 9 . Briefly, protein lysates from hDPCs were prepared using radio immunoprecipitation assay cell lysis buffer containing protease inhibitor cocktail.…”

Section: Western Blot Analysismentioning

confidence: 99%

Synthesized Ceramide Induces Growth of Dermal Papilla Cells with Potential Contribution to Hair Growth

Jeong

Kim

et al. 2019

Ann Dermatol

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Background: The ceramide is known to play an important role in the formation of intracellular lipids, and play a crucial role as a barrier for skin and hair cuticle. Recent study has revealed that ceramide has potential effect on hair growth in a mouse model. However, the role of ceramide in human dermal papilla cells (hDPCs) known to play an important role in hair growth is not well understood yet. Objective: The goal of this study was to investigate the effect of synthetic ceramides (oleyl and stearyl ceramides) on hair growth using hDPCs. Methods: hDPCs were treated with synthesized ceramides. hDPCs viability was evaluated by MTT assay. The expression of hair growth related factors were investigated by western blot, real-time polymerase chain reaction and growth factor array. The expression of β-catenin was confirmed by immunofluorescence. Results: Treatment with ceramides increased the expression of proteins affecting cell proliferation such as Bcl-2, BAX, phosphorylated-ERK and Cyclin D1. Also, ceramides treatment were increased the expression of several growth factors, including epidermal growth factor family, and promote the expression of Wnt/ β-catenin and BMP2/4 signaling. Conclusion: Our data suggest that synthetic ceramides stimulates hair growth by induction proliferation of hDPCs via modulation of Wnt/β-catenin and BMP2/4 signaling. (Ann Dermatol 31(2) 164∼

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Effect of mycophenolic acid on proliferation of dermal papilla cells and induction of anagen hair follicles

Abstract: MPA promotes proliferation of DPCs and induction of anagen hair follicles in mice. This finding raises the possibility that MPA could be used as a treatment option for hair-loss disorders.

Cited by 16 publications

References 20 publications

Review of Hair Follicle Dermal Papilla cells asin vitroscreening model for hair growth

Review of Hair Follicle Dermal Papilla cells asin vitroscreening model for hair growth

Cosmetic Procedures in the Treatment of Alopecia

Synthesized Ceramide Induces Growth of Dermal Papilla Cells with Potential Contribution to Hair Growth

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