Synthesized Ceramide Induces Growth of Dermal Papilla Cells with Potential Contribution to Hair Growth

Oh, Jee Hye; Jeong, Kwan; Kim, Jung Eun; Kang, Hoon

doi:10.5021/ad.2019.31.2.164

Cited by 8 publications

(6 citation statements)

References 35 publications

(40 reference statements)

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“…Specifically, we found that the levels of DAG and ceramide at the plasma membrane decreased after activation of Wnt/β‐catenin signaling with Wnt3a treatment (Table S1 ). Both DAG and ceramide have been associated with Wnt signaling in developmental, homeostatic, and tumorigenic processes [ 19 , 45 , 46 , 47 ]. The binding of the canonical Wnt ligand to the Fz receptor at the plasma membrane causes endocytic internalization of the Wnt‐receptor complex and co‐internalization of the membrane lipids that are found in the vicinity of the Wnt‐receptor complex [ 17 ].…”

Section: Resultsmentioning

confidence: 99%

Comparative membrane lipidomics of hepatocellular carcinoma cells reveals diacylglycerol and ceramide as key regulators of Wnt/β‐catenin signaling and tumor growth

Azbazdar,

Demirci,

Heger

et al. 2023

Molecular Oncology

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Hepatocellular carcinoma (HCC) is largely associated with aberrant activation of Wnt/β‐catenin signaling. Nevertheless, how membrane lipid composition is altered in HCC cells with abnormal Wnt signaling remains elusive. Here, by exploiting comprehensive lipidome profiling, we unravel the membrane lipid composition of six different HCC cell lines with mutations in components of Wnt/β‐catenin signaling, leading to differences in their endogenous signaling activity. Among the differentially regulated lipids are diacylglycerol (DAG) and ceramide, which were downregulated at the membrane of HCC cells after Wnt3a treatment. DAG and ceramide enhanced Wnt/β‐catenin signaling by inducing caveolin‐mediated endocytosis of the canonical Wnt‐receptor complex, while their depletion suppressed the signaling activity along with a reduction of caveolin‐mediated endocytosis in SNU475 and HepG2 cells. Moreover, depletion of DAG and ceramide significantly impeded the proliferation, tumor growth, and in vivo migration capacity of SNU475 and HepG2 cells. This study, by pioneering plasma membrane lipidome profiling in HCC cells, exhibits the remarkable potential of lipids to correct dysregulated signaling pathways in cancer and stop abnormal tumor growth.

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Section: Resultsmentioning

confidence: 99%

Comparative membrane lipidomics of hepatocellular carcinoma cells reveals diacylglycerol and ceramide as key regulators of Wnt/β‐catenin signaling and tumor growth

Azbazdar,

Demirci,

Heger

et al. 2023

Molecular Oncology

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“…Cer is an essential component of the physical barrier of skin. It has been reported that synthetic Cer may induce the proliferation of human dermal papilla cells by regulating the Wnt/β-catenin and BMP2/4 signaling pathways, thereby stimulating hair growth [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Serum lipidomic changes and sex differences in androgenetic alopecia

Wang,

Li,

Qin

et al. 2024

Heliyon

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“…Moreover, the IHC results showed that CGE topical treatment increased β-catenin expression in HF tissue compared to the Veh group. Previous studies elaborated on the crosstalk between β-catenin and Akt/ERK1/2 pathways, and their involvement in hair growth promotion [ 42 – 44 ], and β-catenin expression is promoted by pGSK3β-pAKT-pERK signaling in hDPCs [ 45 ]. A previous study reported that β-catenin regulates SHH expression, and the deletion of β-catenin inhibited SHH expression and inhibited anagen induction [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

The effects of centipedegrass extract on hair growth via promotion of anagen inductive activity

et al. 2022

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To investigate the CGE on hair growth and to explore the mechanism that is involved in the acceleration of anagen induction, we investigated the effects of CGE studied on cell proliferation and molecular mechanism in human hair dermal papilla cells (hDPCs) and keratinocytes (HaCaT cells). Additionally, hair growth evaluation was carried out following topical treatment of the dorsal skin of telogen C57BL/6 mice with CGE for 14 days. As result, CGE increased cell viability and ALP activity in hDPCs. Moreover, CGE increased the expression of catenin beta 1 (CTNNB1), ALP, sex-determining region Y-box 2 (SOX2), insulin-like growth factor 1 (IGF1), and vascular endothelial growth factor A (VEGFA) genes in hDPCs. CGE increased the expression of proteins such as ALP, β-catenin, and phosphorylation of glycogen synthase kinase 3β (pGSK3β), and protein kinase B (pAKT) in hDPCs. Furthermore, CGE induced the proliferation of HaCaT cells and up-regulated AKT-ERK-GSKβ-β-catenin signaling in HaCaT cells. Additionally, the anagen induction effects of CGE were confirmed on the telogen-anagen transition mice model. these findings demonstrated that CGE promoted the entering the growth phase of hair follicle via activation of β-catenin signaling pathways in vivo. Thus, this study suggests that CGE might be a potential therapeutic reagent for hair growth.

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Synthesized Ceramide Induces Growth of Dermal Papilla Cells with Potential Contribution to Hair Growth

Cited by 8 publications

References 35 publications

Comparative membrane lipidomics of hepatocellular carcinoma cells reveals diacylglycerol and ceramide as key regulators of Wnt/β‐catenin signaling and tumor growth

Comparative membrane lipidomics of hepatocellular carcinoma cells reveals diacylglycerol and ceramide as key regulators of Wnt/β‐catenin signaling and tumor growth

Serum lipidomic changes and sex differences in androgenetic alopecia

The effects of centipedegrass extract on hair growth via promotion of anagen inductive activity

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