Effect of morphokinetics and morphological dynamics of cleavage stage on embryo developmental potential: A time-lapse study

Yang, Shiao-Hsuan; Wu, Cheng-Hsuan; Chen, Yu-Ching; Yang, Chueh-Ko; Wu, T. H.; Chen, Pei-Chi; Tsai, Horng‐Der

doi:10.1016/j.tjog.2017.12.013

Cited by 34 publications

(25 citation statements)

References 31 publications

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“…This group also had poorer embryo quality [29]. Our findings are in agreement with previous data that demonstrated faster cleavage achieves more clinical pregnancies and that embryos that develop more quickly seem to exhibit more developmental potential [30].…”

Section: Discussionsupporting

confidence: 92%

Lipid droplets in granulosa cells are correlated with reduced pregnancy rates

et al. 2020

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Background: Lipids are an important source for energy production during oocyte maturation. The accumulation of intracellular lipids binds to proteins to form lipid droplets. This may lead to cellular lipotoxicity. The impact of lipotoxicity on cumulus and granulosa cells has been reported. This pilot study evaluated their correlation to oocyte and embryo quality. Design: Prospective case-control study. Setting: Referral IVF unit. Patients: Women younger than age 40, undergoing IVF with intracytoplasmic sperm injection. Interventions: 15 women with BMI > 30 (high BMI) and 26 women with BMI < 25 (low BMI) were enrolled. IVF outcomes were compared between groups based on BMI. Lipid content in cumulus and granulosa cells was evaluated using quantitative and descriptive methods. Lipid profile, hormonal profile and C-reactive protein were evaluated in blood and follicular fluid samples. Demographic and treatment data, as well as pregnancy rates were collected from electronic medical records. Results: Higher levels of LDL and CRP, slower cell division rate and lower embryo quality were found in the group with high BMI. There was no difference in pregnancy rates between groups. In light of these findings, treatment outcomes were reanalyzed according to patients who became pregnant and those who did not. We found that patients who conceived had significantly lower fat content in the granulosa cells, reflected by mean fluorescence intensity recorded by flow cytometry analysis (23,404 vs. 9370, P = 0.03). Conclusions: BMI has no effect on lipid content in cumulus and granulosa cells, and does not affect likelihood of pregnancy. However, women who achieved pregnancy, regardless of their BMI, had lower lipid levels in their granulosa cells. This finding is important and further study is needed to evaluate lipid content in granulosa cells as a potential predictor of IVF treatment success.

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Section: Discussionsupporting

confidence: 92%

Lipid droplets in granulosa cells are correlated with reduced pregnancy rates

et al. 2020

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“…The most striking example is a particularly short interval in the first cell cycle (<5 hours) that can indicate direct uneven cleavage (DUC). Direct uneven cleavage significantly reduces the chances of an embryo for implantation (4,8,16,20,22,36,45).…”

Section: Discussionmentioning

confidence: 99%

“…Diverse algorithms, using embryo cleavage kinetics available in TLS technology-based imaging, have been proposed in the literature for embryo selection (3,4,7,8,14,(21)(22)(23)(24)(25)(26)(27)(28). Rienzi et al (29) investigated the morphodynamic characterization of euploid blastocyst development under specific culture conditions at 2 different centers.…”

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confidence: 99%

Improving embryo selection by the development of a laboratory-adapted time-lapse model

et al. 2021

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Objective: To study whether a powerful, in-house, embryo-selection model can be developed for a specific in vitro fertilization (IVF) laboratory where embryos were already selected for transfer using general models. Design: In total, 12,944 fertilized oocytes were incubated in an EmbryoScope (Vitrolife, G€ oteborg, Sweden) at our laboratory. Embryos were selected for transfer or freezing using general models. There were 1,879 embryos with known implantation data (KID), of which 425 had positive KIDs. For the outcome, we set 3 endpoints for KID's definition: gestational sac, clinical pregnancy, and live birth. Results of a comparison between KID-positive and -negative embryos for cell division timings were analyzed separately for intracytoplasmic sperm injection (ICSI) and IVF embryos in patients aged 18-41 years. Setting: IVF center. Patients: The study included 1,075 women undergoing IVF or ICSI treatment between June 2013 and February 2019. Intervention(s): None. Main Outcome Measure(s): The KID-positive and -negative embryos were analyzed for statistical differences in cell division timing and cell cycle intervals. We used the EmbryoScope Stats software (Unisense FertiliTech, Aarhus, Denmark) for model development. The statistically different timing parameters were tested for their contribution to scoring in the model. The algorithms were tested for area under the receiver operating characteristic curve (AUC) in the KID embryos for developing day-2, -3, and -5 embryo-selection models. The validation of these algorithms was performed using calibration/validation procedures. Results: Because significant differences in morphokinetics were found between the KID-positive and KID-negative embryos in our laboratory, it was possible to use our specific KID data to develop an in-house model. The algorithms were developed for embryo selection on days 2, 3, and 5 in the ICSI embryos. In most cases, AUC was >0.65, which indicated that these models were valid in our laboratory. In addition, these AUC values were obtained from all gestational sac, clinical pregnancy, and live birth KID embryo databases tested. An increase in the predictability of the models was observed from days 2-3 to day 5 models. The AUC test results ranged between 0.657 and 0.673 for day 2 and day 3, respectively, and 0.803 for the day 5 model. Conclusion:A model based on laboratory-specific morphokinetics was found to be complementary to general models and an important additive tool for improving single embryo selection. Developing an in-house laboratory-specific model requires many stages of sorting and characterization. Many insights were drawn about the model developing process. These may facilitate and improve the process in other laboratories. (Fertil Steril Sci Ò 2021;2:176-97. Ó2021 by American Society for Reproductive Medicine.

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“…The recorded time‐lapse video was used to retrospectively annotate all the variables needed in the study. The definition of each parameter is presented in Table 1, which was cited from previous studies (Basile et al., 2015; Meseguer et al., 2011; Yang et al., 2018). Due to the varying times of each spermatozoa being injected into the mature oocytes, all cell kinetic time points were normalised according to previous studies to minimise standard deviations between the data obtained (Bodri et al., 2015; Kramer et al., 2014).…”

Section: Methodsmentioning

confidence: 99%

Morphokinetics of embryos after IMSI versus ICSI in couples with sub‐optimal sperm quality: A time‐lapse study

Boediono

Handayani²,

Sari³

et al. 2021

Andrologia

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An investigation was conducted to determine the influence of two sperm selection modalities, IMSI and ICSI, on the morphokinetics, dynamic development and ploidy status of embryos derived from males with sub‐optimal sperm profiles during IVF program. A total of 209 PGTA‐tested top‐quality blastocysts (IMSI = 129, ICSI = 80) from 84 couples (IMSI = 51, ICSI = 33) were assessed retrospectively. This study found that both IMSI and ICSI yielded comparable embryo morphokinetics, except for the T7, TEB and CC3 parameters (p < 0.05). A significant lower incidence of multinucleation was observed in the IMSI group when compared to the ICSI group (48.8% vs. 71.3%, p = 0.002), while other parameters of embryo development such as direct cleavage, distorted cytoplasmic movement, reverse cleavage and vacuole(s) appearance did not differ (p > 0.05). No differences were noticed in the proportion of generating chromosomally euploid embryos (44.2% vs. 51.3%, p = 0.394, respectively, for IMSI and ICSI). The implementation of IMSI or ICSI in couples with sub‐optimal sperm profiles resulted in embryos with comparatively similar morphokinetics. Furthermore, the incidence of multinucleation at the two‐ to four‐cell stage was lower following the practice of IMSI, although the method did not improve the proportion of gaining euploid embryos.

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Effect of morphokinetics and morphological dynamics of cleavage stage on embryo developmental potential: A time-lapse study

Abstract: We propose a hierarchical classification system for blastocyst formation prediction, which provides information for embryo selection by using a time-lapse imaging system.

Cited by 34 publications

References 31 publications

Lipid droplets in granulosa cells are correlated with reduced pregnancy rates

Lipid droplets in granulosa cells are correlated with reduced pregnancy rates

Improving embryo selection by the development of a laboratory-adapted time-lapse model

Morphokinetics of embryos after IMSI versus ICSI in couples with sub‐optimal sperm quality: A time‐lapse study

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