Effect of Molybdenum Nanoparticles on Blood Cells, Liver Enzymes, and Sexual Hormones in Male Rats

Abstract

Despite an increasing surge in application of nanoparticles in industries, there is a serious lack of information concerning their impact on human health and the environment. The present study investigated effects of molybdenum nanoparticles (Mo NPs) injected intraperitoneally into Sprague-Dawley rats at different doses of Mo NPs (5, 10, and 15 mg/kg BW per day) during a period of 28 days. Hematological and biochemical parameters as well as sexual hormones and histopathological examinations of the liver and testis were assessed and compared with control group. The results showed that the serum levels of testosterone decreased significantly in both groups of 10 and 15 mg (Mo NPs)/kg BW in comparison with the control group (p < 0.05). However, there were insignificant differences observed in luteinizing hormone (LH) levels and hematological parameters when compared with the control group (p > 0.05). The results of liver enzymes showed that serum levels of aspartate aminotransferase (AST) decreased significantly in both dosage groups of 5 and 10 mg/kg BW (Mo NPs) when compared with the control group (p < 0.05), and significant decrease obtained in lactate dehydrogenase (LDH) levels at dose of 5 mg/kg BW in comparison with the control group (p < 0.05). The histopathological examination of testis showed a decrease in number of Leydig cells. Also, the number of chronic inflammatory cells increased in portal triad and parenchyma in liver tissue of rats exposed to Mo NPs.

Effect of Molybdenum Trioxide Nanoparticles (MoO3 NPs) on Thyroid Hormones in Female Rats

“…According to recent publications, Mo NPs could induce cytotoxicity, genotoxicity, and oxidative stress in mouse skin fibroblast cells and show cytoprotective effects in MCF-7 and HT-1080 cells [11,12]. Also in our previous work we found that the Mo NPs induced a reduction in the serum levels of testosterone in male rats, and histopathology of testis and liver showed a decrease in number of Leydig cells and an increase in chronic inflammatory cells respectively [13]. The aim of this study was to investigate the effect of MoO3 NPs exposure on serum levels of thyroid hormones in female rats.…”

“…Furthermore, it has been reported that Mo NPs significantly protect these and ZnO NPs In our previous study, our in vivo results, suggest that Mo NPs are toxic effect on serum levels of testosterone, AST, and LDH. However, there seemed to be no significant impact on the serum LH levels and hematological parameters [13]. Nano structures can move from body's entry portals into the circulatory and lymphatic systems, and finally into tissues and organs because of their very small sizes.…”

Biological effects of molybdenum compounds in nanosized forms under <i>in vitro</i> and <i>in vivo</i> conditions

“…The cytotoxicity of nanoparticles was assessed using the CCK-8 kit. After 24-h incubation, no cytotoxic effect was IJOMEH 2020;33 1Asadi et al [69] investigated the influence of MoNPs (no physicochemical characteristics of the nanoparticles was provided) on male Sprague-Dawley rats. The NPs were administered intraperitoneally in three concentrations: 5, 10 and 15 mg/kg daily for 28 days.…”

3D printing of Mo-containing scaffolds with activated anabolic responses and bi-lineage bioactivities

“…When Mo enters organisms, it can make up several Mo-enzymes such as aldehyde oxidase (AO), xanthine oxidase (XO), sulfite oxidase (SO), and nitrate reductase (NR). These Mo-enzymes are involved in various biochemical reactions through combining with respective substrates and play important roles in physiology 29 - 33 . In recent years, some metal alloys containing Mo such as Co-Cr-Mo have been found to possess excellent mechanical properties and good biocompatibility, and have been widely used as orthopedic and dental implants 34 - 36 .…”