Effect of Molecular Weight and Molar Ratio of Dextran on Self‐Assembly of Dextran Stearate Polymeric Micelles as Nanocarriers for Etoposide

Abstract

Amphiphilic polymer surfactants are composed of hydrophilic and hydrophobic polymers and are widely used in targeted drug delivery. The purpose of this study was the evaluation of the effect of molecular weight and molar ratio of dextran on physicochemical properties of dextran stearate polymeric micelles. Dextran stearate was synthesized by acylation of dextran with stearoyl chloride. Etoposide loaded polymeric micelles were prepared by dialysis method. The resulting micelles were evaluated for particle size, zeta potential, critical micelle concentration (CMC), drug loading capacity, and release efficiency. Cytotoxicity and cellular uptake of micelles were studied in CT-26 colorectal carcinoma cell line. Molecular weight and molar ratio of dextran-stearate were impressive on zeta potential, CMC, drug loading capacity, and release efficiency. Unlike polymer molecular weight, molar ratio of stearate had a significant effect on cytotoxicity and particle size of etoposide loaded micelles. Although molecular weight of dextran had no significant effect on cytotoxicity of micelles on CT-26 cells, it had drastic attributes for stability of polymeric micelles. Consequently, both variables of molecular weight of dextran and molar ratio of stearate should be taken into account to have a stable and effective micelle of dextran-stearate.

Uptake of Etoposide in CT-26 Cells of Colorectal Cancer Using Folate Targeted Dextran Stearate Polymeric Micelles

“…The method reported in our previous work was used for this synthesis [ 15 ]. Briefly, 1 g of dextran molecular weight (Mw) 6000 (0.166 mmol) was added to 20 mL DMF and stirred at 120°C for 2 h under nitrogen atmosphere.…”

“…Stearate grafted dextran has been synthesized by Du et al [ 14 ] via an esterification reaction and is used to carry doxorubicin to nude mice bearing A549 human lung adenocarcinoma. The effect of molecular weight and molar ratio of dextran on self-assembly of dextran stearate polymeric micelles was further studied by Varshosaz et al [ 15 ]. In vitro cytotoxicity tests showed good toxic effect against drug-sensitive tumor cells while the polymer itself was nontoxic [ 15 ].…”

“…The effect of molecular weight and molar ratio of dextran on self-assembly of dextran stearate polymeric micelles was further studied by Varshosaz et al [ 15 ]. In vitro cytotoxicity tests showed good toxic effect against drug-sensitive tumor cells while the polymer itself was nontoxic [ 15 ]. Moreover, these micelles presented reversal activity against doxorubicin-resistant cells.…”

Self‐Assembly, Interfacial Nanostructure, and Supramolecular Chirality of the Langmuir‐Blodgett Films of Some Schiff Base Derivatives without Alkyl Chain

“…This might be related to the different stereo packing structures of the metal complexes. In addition, these results will expand the research areas for the special amphiphiles without alkyl chain and give some clues to prepare new nanomaterials and nanocomposites [35][36][37][38].…”

Self-assembling polymer systems for advanced treatment of cancer and inflammation

“…A review of this was reported by Goodarzi et at [257]. In recent years, DEX nanocarriers for cancer therapy have been developed based on the modification of DEX to obtain amphiphilic derivatives that self--assemble into micellar or nanoparticulate systems.Varshosaz et al[315] prepared DEX stearate PM as carriers of etoposide. The composition was tuned by changing MW of DEX and molar ratio of stearate.…”