Effect of moisture content on vitreous matrix formation of maize kernel in normal, opaque-2 and qpm germplasm

Sethi, Mehak; Kaur, Harmanjot; Chaudhary, Dharam Paul

doi:10.5958/2395-146x.2020.00002.2

Cited by 2 publications

(3 citation statements)

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“…The modification score represents the degree of opaqueness, namely, 1 for normal lines (0% opaqueness), 5 (100%) for opaque‐2 , and 2 (25%) for QPM lines (Tandzi et al, 2017). Similarly, the moisture content varied from 12.5% to 26.7% among normal, opaque‐2 , and QPM lines, with the least moisture content in QPM and the maximum moisture content in opaque‐2 (Sethi, Kaur, et al, 2020). Specific gravity, 100 kernel weight, and yield potential were the highest in normal, intermediate in QPM, and the lowest in opaque‐2 lines.…”

Section: Resultsmentioning

confidence: 99%

“…Physiological traits of normal, opaque‐2 , and QPM lines were studied, and it was reported that normal lines have high yield potential, 100 kernel weight, and specific gravity with 0% opaqueness, whereas the opaque‐2 mutation leads to 100% opaqueness, which distorts the starch protein matrix and subsequently increases the moisture content (Sethi et al, 2020); however, 100 kernel weight and specific gravity decrease in opaque‐2 lines and the opaque‐2 mutation is also known to negatively influence the yield potential. In QPM, introgression of endosperm modifiers leads to retention of the vitreous kernel texture and moisture content decreases, whereas 100 kernel weight, specific gravity, and yield potential are higher than those of opaque‐2 .…”

Section: Discussionmentioning

confidence: 99%

“…Concomitantly, biochemical parameters including total protein, zein content, nonzein content, and prolamin‐like content were also recorded for 2 years in three replicates. The procedure for all the physiological and biochemical parameters mentioned above was followed according to previous studies (Sethi, Kaur, et al, 2020; Sethi, Kumar, et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

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Elucidation of zein isoforms associated with high protein quality traits for targeted improvement in maize‐based nutrition

Sethi,

Singh,

Garg

et al. 2023

Cereal Chem

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Background and ObjectivesZein proteins of maize endosperm are nutritionally poor, but important for vitreous kernel texture. The Opaque‐2 mutation enhances the protein quality but downregulates zein expression, distorting the kernel texture. Quality protein maize (QPM) is nutritionally improved, hard endosperm maize developed by introgression of endosperm modifiers under an opaque‐2 background. The present study aims to analyze the variability in zein expression patterns in normal, opaque‐2, and QPM lines through sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and two‐dimensional gel electrophoresis analyses.FindingsResults revealed that the total number of zein isoforms is almost similar in opaque‐2, normal, and QPM lines. Overall, in this study, it is concluded that the opaque‐2 mutation specifically affects the expression of high‐molecular‐weight zeins, which are being counterbalanced by the expression of low‐molecular‐weight zein isoforms and nonzein proteins.ConclusionGenomic data retrieval studies revealed the possible presence of multiple 27 kDa isoforms with varied isoelectric points (pI), which is a subject for further investigation. The differential expression of 15 kDa zein in QPM emerged as a novel player in endosperm modification.Significance and NoveltyThe present study provides insight into the diversified expression of zeins in different maize types. The variable isoform expression has the potential to generate stable QPM lines by targeting effective isoforms, ensuring endosperm modification without hampering the nutritional quality.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%