Effect of Modified Nonequilibrium Plasma with Chlorhexidine Digluconate against Endodontic Biofilms In Vitro

Du, Tianfeng; Shi, Qi; Shen, Ya; Cao, Yingguang; Ma, Jia; Lü, Xinpei; Xiong, Zilan; Haapasalo, Markus

doi:10.1016/j.joen.2013.06.027

Cited by 48 publications

(41 citation statements)

References 34 publications

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“…Studies have shown that atmospheric pressure cold plasma had a high efficiency in killing the bacteria in Enterococcus faecalis biofilms in in vitro root canal treatment [14,15] . Du et al [3] reported that modified nonequilibrium plasma with 2% CHX was more effective in disinfecting the in vitro bacterial biofilms than the conventional nonequilibrium plasma. However, the biosecurity of the plasma was still unknown.…”

Section: Discussionmentioning

confidence: 99%

“…However, studies have shown that it was difficult to completely eradicate microbes in the root canal via endodontic treatment [1] . For this reason, lots of new methods such as laser, ultrasound, antimicrobial photodynamic therapy, and nanoparticles were introduced, including nonequilibrium plasma that was widely used in oral clinic [2][3][4][5][6] . Studies have shown that atmospheric pressure non-equilibrium plasmas (APNPS) can effectively kill the most common pathogenic bacteria in Enterococcus faecalis biofilms in root canal [2] , meanwhile, a research about combination of nonequilibrium plasma with 2% chlorhexidine digluconate (CHX) indicated that modified nonequilibrium plasma was more effective in killing bacteria in the single biofilms (Enterococcus faecalis biofilms) and multispecies biofilms than the conventional nonequilibrium plasma [3] .…”

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confidence: 99%

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Cytotoxicity of modified nonequilibrium plasma with chlorhexidine digluconate on primary cultured human gingival fibroblasts

Chen

Shi

Qing

et al. 2016

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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The aim of this study was to investigate the cytotoxicity of modified nonequilibrium plasma with chlorhexidine digluconate (CHX) on human gingival fibroblasts (HGFs), and to evaluate the biosecurity of modified nonequilibrium plasma with 2% CHX as a new method of root canal treatment. Tissue samples taken from human gingiva were primarily cultured and passaged. Cells from passages 3-7 were used. HGFs were treated by modified nonequilibrium plasma with 2% CHX for 0 min (control group), 30 s, 1 min, 1.5 min, 3 min, 5 min, and 10 min, respectively, and then they were incubated for 0, 24, and 48 h. After that, cell counting kit-8 (CCK-8) assay was applied to analyze the cytotoxicity of modified nonequilibrium plasma with 2% CHX on HGFs. There was no significant difference between the 0 h group treated with the modified nonequilibrium plasma for 1 min and the control group (P>0.05). However, there were significant differences between all the other treated groups and the control group (P<0.05). When treated for 1.5 min or shorter, the cell viability was obviously increased; while treated for 3 min or longer, it was obviously reduced. Moreover, when successively cultured for 0, 24, and 48 h, cell viability was decreased at first and then increased in the 3-min-treated and 5-min-treated groups. The modified nonequilibrium plasma with 2% CHX was of no influence on cell viability in 1.5 min treatment, and it could be safely used on root canal treatment.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Cytotoxicity of modified nonequilibrium plasma with chlorhexidine digluconate on primary cultured human gingival fibroblasts

Chen

Shi

Qing

et al. 2016

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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“…1) 11,12) . However, there are few reports about its application for sterilization in the field of dentistry [13][14][15][16] . Yamazaki et al 17) have previously reported the sterilization effects of plasma exposure in an acidic environment (the reduced-pH method) for a wide variety of oral pathogenic microorganisms, both in suspension and in biofilms.…”

Section: Introductionmentioning

confidence: 99%

Plasma-treated water eliminates <i>Streptococcus mutans</i> in infected dentin model

et al. 2017

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Non-mechanical procedures for removing caries-infected dentin are warranted in dentistry. We previously demonstrated the marked sterilization effect for direct irradiation of low-temperature plasma using dentin model infected with Streptococcus mutans. However it requires 180 s of intraoral plasma irradiation to eliminate bacteria. We alternatively investigated whether plasma-treated water (PTW), i.e., pure water exposed to plasma in an atmosphere, has a same bactericidal activity with the plasma irradiation. In the infected dentin model, the viable S. mutans counts recovered by bur at depth of 0.8-2.4 mm from the cavity floor were 10 4 -10 6 CFU/ round bur. After PTW application for only 10 s, the count was significantly decreased to below the detection limit (2.5 CFU/round bur) or 3.0±5.0 CFU/round bur. Since the bactericidal activity of PTW is rapidly deactivated at body temperature (37°C), PTW is likely to be biocompatible and holds significant potential for non-mechanical procedures for removing caries-infected dentin.

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“…Its dental applications, including oral microorganism inactivation [7][8][9][10][11][12][13][14], tooth whitening [15][16][17][18][19], dental material modification [20][21][22] and oral cancer treatment [23] have been reported. Its efficacy in inactivating microorganisms reminds us of its potential application in dealing with oral inflammatory process.…”

Section: Introductionmentioning

confidence: 99%

Inactivation of Candida albicans Biofilms on Polymethyl Methacrylate and Enhancement of the Drug Susceptibility by Cold Ar/O2 Plasma Jet

Wang

Sun

Pan

et al. 2015

Plasma Chem Plasma Process

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The antimicrobial effects of the cold plasma for the dental pathogenic microorganism propose a promising approach to the Denture Stomatitis (DS) treatment. However, it is crucial to understand that the complexity of the biofilm microenvironment may compromise the efficiency of the therapy. As one of the major issue for DS, Candida albicans biofilms (ATCC10231) formed on denture base resins were treated by cold Ar/O 2 (2 %) plasma jet. Spatial viability of the biofilms was investigated with confocal scanning laser microscopy through evaluating their inside cross-section properties. Results showed Candida albicans biofilms with thickness of *100 lm was completely inactivated by 8 min plasma treatment. Morphology change of the fungi was also observed by the scanning electron microscopy. Drug susceptibilities, the sessile minimum inhibitory concentration (SMIC50) of the biofilm for amphotericin B and fluconazole were decreased from [32 and [256 lg/mL to 8 and 64 lg/mL after 1 min's plasma treatment, respectively. The reactive species produced from plasma were monitored by optical emission spectroscopy. The successfully inactivation of Candida albicans biofilms and the significant enhancement of its drug susceptibilities induced by the plasma released reactive species propose a promising strategy for the treatment of DS caused by drug-resistant Candida albicans biofilms.

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Effect of Modified Nonequilibrium Plasma with Chlorhexidine Digluconate against Endodontic Biofilms In Vitro

Cited by 48 publications

References 34 publications

Cytotoxicity of modified nonequilibrium plasma with chlorhexidine digluconate on primary cultured human gingival fibroblasts

Cytotoxicity of modified nonequilibrium plasma with chlorhexidine digluconate on primary cultured human gingival fibroblasts

Plasma-treated water eliminates <i>Streptococcus mutans</i> in infected dentin model

Inactivation of Candida albicans Biofilms on Polymethyl Methacrylate and Enhancement of the Drug Susceptibility by Cold Ar/O2 Plasma Jet

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