Effect of Mirasol pathogen reduction technology system on in vitro quality of MCS+ apheresis platelets

In vitro study of platelet function confirms the contribution of the ultraviolet B (UVB) radiation in the lesions observed in riboflavin/UVB‐treated platelet concentrates

“…A change of ATP consumption rate is another hypothesis for the increased glycolytic flux in riboflavin/UVB‐treated PLTs. Increased expression of P‐selectin and lower ATP content were reported in riboflavin/UVB‐treated PLTs . We also found an increased expression of CD62P, active GPIIbIIIa at the surface of the PLTs, and an increased adhesion to fibrinogen indicating that the riboflavin/UVB‐treated PLTs were somehow activated upon treatment.…”

“…The results from this study reveal that the riboflavin/UVB treatment significantly impacts the metabolism of PLTs as evidenced by higher glucose consumption and lactate production rates. This phenomenon has been observed by other groups in Mirasol‐treated PLTs . It has been hypothesized that the increased anaerobic glycolysis rate in riboflavin/UVB‐treated PLTs was either due 1) to an impaired mitochondrial functionality leading to a decrease of oxidative phosphorylation, 2) to an alteration of the PLT storage medium characteristics (pH, oxygen content, plasma protein content), or 3) to changes in ATP consumption rate …”

“…This phenomenon has been observed by other groups in Mirasol-treated PLTs. 21,36,[42][43][44][45][46][47][48][49][50][51][52][53][54][55][56] It has been hypothesized that the increased anaerobic glycolysis rate in riboflavin/UVB-treated PLTs was either due 1) to an impaired mitochondrial functionality leading to a decrease of oxidative phosphorylation, 2) to an alteration of the PLT storage medium characteristics (pH, oxygen content, plasma protein content), or 3) to changes in ATP consumption rate. 46 During storage at 22 C PLTs use the anaerobic pathway of glycolysis for energy production only to a minor extent (approx.…”

“…In vitro studies reported metabolic changes, impaired mitochondrial function, accelerated passive activation, and altered agonist‐induced PLT aggregation in INTERCEPT‐treated compared to untreated PLTs . Mirasol‐treated PLTs exhibit an increased expression of P‐selectin (CD62P), higher lactate production, and increased glucose and oxygen consumption, as well as lower ATP over storage time . The Mirasol PIT leads to hyperactive PLTs resulting in a reduced degranulation capacity upon acute stimulation …”

“…23,[35][36][37][38][39][40][41] Mirasol-treated PLTs exhibit an increased expression of P-selectin (CD62P), higher lactate production, and increased glucose and oxygen consumption, as well as lower ATP over storage time. 21,[42][43][44][45][46][47][48][49][50][51][52][53][54][55] The Mirasol PIT leads to hyperactive PLTs resulting in a reduced degranulation capacity upon acute stimulation. 56 Both amotosalen and riboflavin are photosensitizers able to directly oxidize other molecules and generate ROS thereby potentially triggering other damages to the cells.…”