Effect of Mineral Salts Addition on the Behaviour of an Ethanol Biofilter

Perez, Waleska; Domenech, F.; Röger, Petra; Christen, Pierre

doi:10.1080/09593332308618347

Cited by 13 publications

(3 citation statements)

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“…The alkane degradation was confirmed by measuring the CO 2 release in the headspace of the microcosms, using thermal conductivity detection gas chromatograph (Shimadzu 8A, Champs‐sur‐Marne, France) with a concentric CTR1 column (Alltech). Operating conditions were as reported by Teran Perez et al. (2002).…”

Section: Methodsmentioning

confidence: 99%

Identification of different alkane hydroxylase systems inRhodococcus ruberstrain SP2B, an hexane-degrading actinomycete

Amouric

Quéméneur

Grossi

et al. 2009

Journal of Applied Microbiology

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Aims: To investigate the alkane‐hydroxylating system of isolate SP2B, closely related to Rhodococcus ruber DSM 43338T and uncharacterized so far for its alkane degradation genes. Methods and Results: Although isolate SP2B and reference strain can grow on by‐products from hexane degradation, the type strain R. ruber was unable, unlike SP2B isolate, to use short‐chain alkanes, as assessed by gas chromatography. Using PCR with specific or degenerated primers, inverse PCR and Southern blot, two alkane hydroxylase encoding genes (alkB) were detected in both bacteria, which is in agreement with their alkane range. The first AlkB was related to Rhodococcus AlkB7 enzymes and contains a nonbulky residue at a specific position, suggesting it might be involved in medium‐ and long‐chain alkane oxidation. The second partial alkB gene potentially belongs to alkB5‐type, which was found in bacteria unable to use hexane. Moreover, a partial P450 cytochrome alkane hydroxylase, thought to be responsible for the hexane degradation, was detected only in the isolated strain. Conclusions: Rhodococcus ruber SP2B should prove to be a promising candidate for bioremediation studies of contaminated sites because of its large degradation range of alkanes. Significance and Impact of the Study: This is the first thorough study on R.ruber alkane degradation systems.

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Section: Methodsmentioning

confidence: 99%

Identification of different alkane hydroxylase systems inRhodococcus ruberstrain SP2B, an hexane-degrading actinomycete

Amouric

Quéméneur

Grossi

et al. 2009

Journal of Applied Microbiology

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“…The CO 2 concentration contained in the outlet of the biofilter and in the headspaces of the microcosms was measured with thermal conductivity detector gas chromatography (Shimadzu, Duisburg, Germany) using a concentric CTR1 column (Alltech ) . Operating conditions were as already reported (Teran Perez et al , 2002). The production rate of CO 2 was expressed as g per m 3 gas phase per h.…”

Section: Methodsmentioning

confidence: 99%

Study of a hexane-degrading consortium in a biofilter and in liquid culture: biodiversity, kinetics and characterization of degrading strains

Amouric

Verhé²,

Auria

et al. 2006

FEMS Microbiology Ecology

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A gasoline-degrading consortium, originating from a Mexican soil, was used to study its hexane-degradation kinetics in liquid culture and in a biofilter with mineral support. The biodiversity of the consortium depending on the culture conditions and electron and energy source (gasoline, hexane in liquid or hexane in the biofilter) was analyzed using a 16S rRNA-based approach. Significant differences between the populations were observed, indicating a probable adaptation to the substrate. Two strains, named SP2B and SP72-3, isolated from the consortium, belonged to Actinomycetes and demonstrated a high metabolic potential in hexane degradation. Even though the SP2B strain was related to Rhodococcus ruber DSM 43338(T) by phylogenetic studies, it displayed enlarged metabolic properties in hexane and other short-alkane degradation compared with the collection strain.

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“…An important criterion that has not been fully resolved is the importance of the form and concentration of the nitrogen source used in enriching for methylotrophs or supporting their growth and activity in liquid culture or in biofiltration applications. The intermittent addition of nitrogen in a mineral salts mixture has been demonstrated to improve the capacity of biofilters in removing ethanol (Teran Perez et al 2002) and toluene (Prado et al 2002). Addition of greater concentration of nutrients appears to be most important when the biofilter is subjected to high contaminant loading rates (Gribbins and Loehr 1998).…”

Section: Introductionmentioning

confidence: 98%

Effect of nitrogen source on methanol oxidation and genetic diversity of methylotrophic mixed cultures enriched from pulp and paper mill biofilms

Babbitt

Lindner

2010

Biodegradation

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Methanol-oxidizing bacteria may play an important role in the development and use of biological treatment systems for the removal of methanol from industrial effluents. Optimization of methanol degradation potential in such systems is contingent on availability of nutrients, such as nitrogen, in the most favorable form and concentration. To that end, this study examined the variation in growth, methanol degradation, and bacterial diversity of two mixed methylotrophic cultures that were provided nitrogen either as ammonium or nitrate and in three different concentrations. Methanol-degrading cultures were enriched from biofilms sampled at a pulp and paper mill and grown in liquid batch culture with methanol as the only carbon source and either ammonium or nitrate as the only added nitrogen source. Results indicate that growth and methanol removal of the mixed cultures increase directly with increased nitrogen, added in either form. However, methanol removal and bacterial diversity, as observed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) methods, were higher when using nitrate as the nitrogen source for enrichment and growth, rather than ammonium. Based on results described here, nitrate may potentially be a better nitrogen source when enriching or working with mixed methylotrophic cultures, and possibly more effective when used as a nutrient addition to biofilters.

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Effect of Mineral Salts Addition on the Behaviour of an Ethanol Biofilter

Cited by 13 publications

References 0 publications

Identification of different alkane hydroxylase systems inRhodococcus ruberstrain SP2B, an hexane-degrading actinomycete

Identification of different alkane hydroxylase systems inRhodococcus ruberstrain SP2B, an hexane-degrading actinomycete

Study of a hexane-degrading consortium in a biofilter and in liquid culture: biodiversity, kinetics and characterization of degrading strains

Effect of nitrogen source on methanol oxidation and genetic diversity of methylotrophic mixed cultures enriched from pulp and paper mill biofilms

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