Effect of Mg&lt;sup&gt;2+&lt;/sup&gt; Addition during HA Nanoparticles Synthesis Using Ultrasonic Irradiation

Gouveia, D.S.; Coutinho, Adriana; Bressiani, Ana Helena A.; Bressiani, José Carlos

doi:10.4028/www.scientific.net/kem.361-363.203

Cited by 4 publications

(5 citation statements)

References 9 publications

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“…11 12 These substitutions do not lead to change the basic structural characteristics of HAp but affects the mechanical properties, crystallization, thermal stability, solubility and bone bonding capability of the apatite materials. [13][14][15][16][17][18][19][20] Generally, two main parameters could affect the solubility of hydroxyapatite at physiological state including: (1) …”

Section: Introductionmentioning

confidence: 99%

Effect of magnesium substitution on structural and biological properties of synthetic hydroxyapatite powder

Mehrjoo¹,

Javadpour²,

Shokrgozar³

et al. 2015

Mat Express

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The calcium phosphate compounds because of the wide variety in medical application, especially in orthopedics and dental surgery, they have generated a great deal of interest. One of these compounds is hydroxyapatite (HAp). In this regard, numerous strategies have been designed to increase the structural properties of HA. Consequently, in the present study, magnesium (Mg 2+ ) ions were incorporated to HA using wet chemical precipitation route in order to form the MgxHAp. In this experiment, Ca(NO 3 ) 2 · 4H 2 O, (NH 4 ) 2 HPO 4 and Mg(NO 3 ) 2 · 6H 2 O were used as starting raw materials. The obtained powders were characterized utilizing X-ray Diffraction (XRD), Fourier Transform Infra-Red (FTIR) spectroscopy and Scanning Electron Microscopy (SEM). The X-ray results demonstrated that the addition of Mg 2+ ions into apatite lattice has decreased the degree of crystallinity in the samples. Moreover, the agglomeration of powders were slightly increased in the presence of Mg 2+ ions. The effect of different amounts of Mg 2+ ions on biological properties of samples were assessed using cellular proliferation, alkaline phosphatase (ALP) activity and gene expression. The cellular behavior of MG-63 cells was improved in Mg 2+ incorporated samples. Finally, the results indicated that the high amounts of Mg 2+ in HAp, could improve the cellular behavior in comparison with pure HAp.

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Section: Introductionmentioning

confidence: 99%

Effect of magnesium substitution on structural and biological properties of synthetic hydroxyapatite powder

Mehrjoo¹,

Javadpour²,

Shokrgozar³

et al. 2015

Mat Express

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“…Some researchers have performed isomorphic substitutions in the hexagonal structure of synthetic HA through partial exchange of Ca by F, Zn, Sr or Mg (Cazalbou et al, 2005;Terra et al, 2009;Lima et al, 2011;Zilm et al, 2015;Geng et al, 2016;Ribeiro et al, 2016;Tao et al, 2016) in order to improve the physicochemical properties of this ceramic and, consequently, the biological response after implantation in vivo. Among these ions, Mg is of great clinical interest, since it is closely correlated with homeostasis and bone mineralization (Bigi et al, 1992(Bigi et al, , 1993Bertoni et al, 1998;Gouveia et al, 2008;Jia et al, 2010;Laurencin et al, 2011;Nabiyouni et al, 2015;Tao et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…These substitutions may alter crystallinity, solubility, network parameters, thermal stability, surface reactivity, bioactivity (Bigi et al, 1992(Bigi et al, , 1993Bertoni et al, 1998;LeGeros, 2002;Gouveia et al, 2008;Terra et al, 2009;Laurencin et al, 2011;Tao et al, 2016), the adsorption properties of the HA (Bigi et al, 1992(Bigi et al, , 1993Bertoni et al, 1998;Mavropoulos, 1999;Laurencin et al, 2011;Tao et al, 2016) and the biological behavior (Lima et al, 2011). Therefore, new studies are needed to evaluate these biomaterials with new physical-chemical characteristics.…”

Section: Introductionmentioning

confidence: 99%

Biological behavior of magnesium-substituted hydroxyapatite during bone repair

et al. 2021

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The aim of this study was to analyze the biological behavior and osteogenic potential of magnesium (Mg) substituted hydroxyapatite (HA) microspheres, implanted in a critical bone defect, considering that this ion is of great clinical interest, since it is closely associated with homeostasis and bone mineralization. For the purpose of this study, 30 rats were used to compose three experimental groups: GI -bone defect filled with HA microspheres; GII -bone defect filled with HA microspheres replaced with Mg; GIII -empty bone defect; evaluated at biological points of 15 and 45 days. The histological results, at 15 days, showed, in all the groups, a discrete chronic inflammatory infiltrate; biomaterials intact and surrounded by connective tissue; and bone neoformation restricted to the borders. At 45 days, in the GI and GII groups, an inflammatory response of discrete granulomatous chronic type was observed, and in the GIII there was a scarce presence of mononuclear inflammatory cells; in GI and GII, the microspheres were seen to be either intact or fragmented, surrounded by fibrous connective tissue rich in blood vessels; and discrete bone neoformation near the edges and surrounding some microspheres. In GIII, the mineralization was limited to the borders and the remaining area was filled by fibrous connective tissue. It was concluded that the biomaterials were biocompatible and osteoconductive, and the percentage of Mg used as replacement ion in the HA did not favor a greater bone neoformation in relation to the HA without the metal. Comportamento biológico de hidroxiapatita substituída com magnésio durante o reparo ósseoResumo O objetivo deste estudo foi analisar o comportamento biológico de microesferas de hidroxiapatita (HA) substituída com magnésio (Mg) durante o reparo de defeito ósseo crítico, tendo em vista que este íon é de grande interesse clínico, pois está intimamente associado à homeostasia e à mineralização óssea. Para tanto, utilizou-se 30 ratos para compor três grupos experimentais: GI -defeito ósseo preenchido com microesferas de HA; GII -defeito ósseo preenchido com microesferas de HA substituída com Mg; GIII (controle) -defeito ósseo vazio; avaliados nos pontos biológicos de 15 e 45 dias. Os resultados histológicos evidenciaram, aos 15 dias, discreto infiltrado inflamatório crônico e neoformação óssea restrita às bordas, em todos os grupos. Nos grupos GI e GII, os biomateriais mantiveram-se íntegros e circundados por tecido conjuntivo frouxo. Aos 45 dias, notou-se resposta inflamatória do tipo crônica granulomatosa discreta nos grupos GI e GII, e no GIII presença escassa de células inflamatórias mononucleares. As microesferas implantadas no GI e GII mantiveram-se, em sua maioria, íntegras e envolvidas por tecido conjuntivo fibroso. Notou-se discreta neoformação óssea próxima às bordas e circunjacente a algumas microesferas. No GIII, a mineralização limitou-se às bordas e a área remanescente foi preenchida por tecido conjuntivo fibroso. Conclui-se que os biomateriais foram biocompatíveis, bioativos...

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“…A HPLG with GMA degree substitution of 10% (w/w) was attained in the derivatization reaction. Hydroxyapatite nanoparticles were synthesized following a method proposed recently by Bressiani et al [8]. The mean particle size of the HA nanocristals as analyzed by a Phillips XL30 scanning electron microscopy (SEM) was 230 + 80 nm.…”

Section: Methodsmentioning

confidence: 99%

A Novel Bone Scaffolds Based on Hyperbranched Polyglycerol Fibers Filled with Hydroxyapatite Nanoparticles: <i>In Vitro</i> Cell Response

Queiroz

Bressiani

et al. 2008

KEM

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A novel bone scaffolding material was successfully fabricated by electrospinning from hyperbranched polyglycerol (HPGL) solutions containing nanoparticles of hydroxyapatite (HA). The potential use of the electrospun fibrous HPGL-HA scaffolds for bone regeneration was evaluated in vitro with human osteoblasts in terms of alkaline phosphatase (ALP) activity of the cells that were cultured directly on the scaffolds. The results were compared with those on corresponding HPLG-HA solution-cast film scaffolds. It was found that all of the fibrous scaffolds promoted much better adhesion and proliferation of cells than the corresponding film scaffolds.

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Effect of Mg<sup>2+</sup> Addition during HA Nanoparticles Synthesis Using Ultrasonic Irradiation

Cited by 4 publications

References 9 publications

Effect of magnesium substitution on structural and biological properties of synthetic hydroxyapatite powder

Effect of magnesium substitution on structural and biological properties of synthetic hydroxyapatite powder

Biological behavior of magnesium-substituted hydroxyapatite during bone repair

A Novel Bone Scaffolds Based on Hyperbranched Polyglycerol Fibers Filled with Hydroxyapatite Nanoparticles: <i>In Vitro</i> Cell Response

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