Effect of magnesium substitution on structural and biological properties of synthetic hydroxyapatite powder

Abstract

The calcium phosphate compounds because of the wide variety in medical application, especially in orthopedics and dental surgery, they have generated a great deal of interest. One of these compounds is hydroxyapatite (HAp). In this regard, numerous strategies have been designed to increase the structural properties of HA. Consequently, in the present study, magnesium (Mg 2+ ) ions were incorporated to HA using wet chemical precipitation route in order to form the MgxHAp. In this experiment, Ca(NO 3 ) 2 · 4H 2 O, (NH 4 ) 2 HPO 4 and Mg(NO 3 ) 2 · 6H 2 O were used as starting raw materials. The obtained powders were characterized utilizing X-ray Diffraction (XRD), Fourier Transform Infra-Red (FTIR) spectroscopy and Scanning Electron Microscopy (SEM). The X-ray results demonstrated that the addition of Mg 2+ ions into apatite lattice has decreased the degree of crystallinity in the samples. Moreover, the agglomeration of powders were slightly increased in the presence of Mg 2+ ions. The effect of different amounts of Mg 2+ ions on biological properties of samples were assessed using cellular proliferation, alkaline phosphatase (ALP) activity and gene expression. The cellular behavior of MG-63 cells was improved in Mg 2+ incorporated samples. Finally, the results indicated that the high amounts of Mg 2+ in HAp, could improve the cellular behavior in comparison with pure HAp.

An overview on biological effects of trace-element in substituted calcium phosphates

“…Higher cellular activity was observed in vitro with materilas containing high level of Mg(II) ions in CaHap lattice [18] in comparison with pure CaHap.…”

“…Enamel, dentin and bone contain, respectively, 0.44, 1.23, and 0.72 wt% of Mg [18]. It is well known that Mg is closely associated with the mineralization of calcified tissues, directly stimulating osteoblast proliferation.…”

Curcumin-Loaded Starch Micro/Nano Particles for Biomedical Application: The Effects of Preparation Parameters on Release Profile

“…After 24 h, MTT assays were performed and the absorbance values of the plates were read at 440 nm using a multiwall microplate reader (STAT FAX 2100, USA). The culture medium was used as control group and curcumin solutions with the same curcumin contents comparing to different drug‐loaded particles (four replicates of each sample) were tested as comparatives …”

Silk as a potential candidate for bone tissue engineering

“…We and many other researchers have reported the extensive applications of ceramics for bone tissue engineering, which is largely due to their ability to promote the adhesion and proliferation of osteoblasts and enhance new bone formation in vitro and in vivo [156][157][158][159][160][161][162][163][164]. McNamara et al have blended SF with HA using three different techniques (Figure 2).…”