Effect of methylene blue photodynamic therapy on human neutrophil functional responses

Trevisan, Elisa; Menegazzi, Renzo; Zabucchi, Giuliano; Troian, Barbara; Prato, Stefano Del; Vita, Francesca; Rapozzi, Valentina; Grandolfo, Micaela; Borelli, Violetta

doi:10.1016/j.jphotobiol.2019.111605

Cited by 12 publications

(11 citation statements)

References 92 publications

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“…All in all, the use of LEDs for PDT was demonstrated in the mid-1990s by Schmid et al in brain tumours of canines treated with porifrin [ 52 ]. Presently, the rapid growth of LEDs led to the development of LED arrays of various wavelengths and handheld systems achieving the high power required for PDT [ 61 , 62 , 63 , 64 ]. For instance, array systems delivering a fluence rate of 100 mW/cm 2 demonstrated a 90% of cell death in ovarian cancer cell line using LED modules reaching a fluence of 50 J/cm 2 [ 62 ].…”

Section: Light Technology For Pdtmentioning

confidence: 99%

Light Technology for Efficient and Effective Photodynamic Therapy: A Critical Review

Algorri

Ochoa

Roldán-Varona

et al. 2021

Cancers

104

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Photodynamic therapy (PDT) is a cancer treatment with strong potential over well-established standard therapies in certain cases. Non-ionising radiation, localisation, possible repeated treatments, and stimulation of immunological response are some of the main beneficial features of PDT. Despite the great potential, its application remains challenging. Limited light penetration depth, non-ideal photosensitisers, complex dosimetry, and complicated implementations in the clinic are some limiting factors hindering the extended use of PDT. To surpass actual technological paradigms, radically new sources, light-based devices, advanced photosensitisers, measurement devices, and innovative application strategies are under extensive investigation. The main aim of this review is to highlight the advantages/pitfalls, technical challenges and opportunities of PDT, with a focus on technologies for light activation of photosensitisers, such as light sources, delivery devices, and systems. In this vein, a broad overview of the current status of superficial, interstitial, and deep PDT modalities—and a critical review of light sources and their effects on the PDT process—are presented. Insight into the technical advancements and remaining challenges of optical sources and light devices is provided from a physical and bioengineering perspective.

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Section: Light Technology For Pdtmentioning

confidence: 99%

Light Technology for Efficient and Effective Photodynamic Therapy: A Critical Review

Algorri

Ochoa

Roldán-Varona

et al. 2021

Cancers

104

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“…The infiltration of neutrophils reached a peak at 4 h. At this time, the expression of E-selectin was also increased significantly, and it was correlated strongly with the number of neutrophils ( 86 ). MB-PDT can increase the adhesion of neutrophils, but not modify the release of myeloperoxidase ( 87 ). Fibroblasts initially responded to PDT by expressing heat shock proteins, triggerring the nuclear factor kappa B pathway, prompting macrophages to secrete pro-inflammatory cytokines [tumor necrosis factor alpha (TNF-α), IL-6, and IL-1β].…”

Section: The Effects Of Photosensitizer-based Therapies On Skin Cellsmentioning

confidence: 99%

The photosensitizer-based therapies enhance the repairing of skin wounds

Ning

Zeng

et al. 2022

Front. Med.

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Wound repair remains a clinical challenge and bacterial infection is a common complication that may significantly delay healing. Therefore, proper and effective wound management is essential. The photosensitizer-based therapies mainly stimulate the photosensitizer to generate reactive oxygen species through appropriate excitation source irradiation, thereby killing pathogenic microorganisms. Moreover, they initiate local immune responses by inducing the recruitment of immune cells as well as the production of proinflammatory cytokines. In addition, these therapies can stimulate the proliferation, migration and differentiation of skin resident cells, and improve the deposition of extracellular matrix; subsequently, they promote the re-epithelialization, angiogenesis, and tissue remodeling. Studies in multiple animal models and human skin wounds have proved that the superior sterilization property and biological effects of photosensitizer-based therapies during different stages of wound repair. In this review, we summarize the recent advances in photosensitizer-based therapies for enhancing tissue regeneration, and suggest more effective therapeutics for patients with skin wounds.

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“…3 Hematoporphyrin derivatives have pioneered the PDT studies, but their hydrophobic nature restricts the efficiency of treatment. 4 Other photosensitizers like porphyrin, 5 porphin, 6 chlorin e6, methylene blue (MB), 7 and metal phthalocyanine 8 were also proposed for the PDT applications. All these photosensitizers have shown improvement in different aspects of PDT performances, but some side effects are also reported.…”

Section: ■ Introductionmentioning

confidence: 99%

Phosphate-Assisted Transformation of Methylene Blue to Red-Emissive Carbon Dots with Enhanced Singlet Oxygen Generation for Photodynamic Therapy

Wang

Ran

et al. 2021

ACS Appl. Nano Mater.

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Photodynamic therapy (PDT) has attracted wide attention due to its distinct advantages in cancer treatment. Herein, a kind of red emissive carbon dots (R-CDs) was synthesized from methylene blue (MB) and phosphate through a hydrothermal method. The resultant R-CDs display good biocompatibility, photostability, and high singlet oxygen ( 1 O 2 ) yield (0.91); thus, they have been successfully applied to the PDT study in vitro. More importantly, the R-CDs show noninfective property to DNA, which is substantially different to their precursor MB. The structure of R-CDs was comprehensively characterized both experimentally as well as by density functional theory (DFT) calculations. This study not only provides a rational strategy for preparation of highly efficient PDT material but also gives insight into the mechanism of 1 O 2 generation.

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Effect of methylene blue photodynamic therapy on human neutrophil functional responses

Cited by 12 publications

References 92 publications

Light Technology for Efficient and Effective Photodynamic Therapy: A Critical Review

Light Technology for Efficient and Effective Photodynamic Therapy: A Critical Review

The photosensitizer-based therapies enhance the repairing of skin wounds

Phosphate-Assisted Transformation of Methylene Blue to Red-Emissive Carbon Dots with Enhanced Singlet Oxygen Generation for Photodynamic Therapy

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