Effect of Methanol Extract ofZanthoxylum piperitumLeaves and of Its Compound, Protocatechuic Acid, on Hepatic Drug Metabolizing Enzymes and Lipid Peroxidation in Rats

Abstract

The effect of methanol extract and protocatechuic acid from the leaves of Zanthoxylum piperitum on lipid peroxidation and drug metabolizing enzymes were investigated in the liver of bromobenzene-treated rats. The methanol extract and protocatechuic acid reduced the level of lipid peroxide induced by bromobenzene. The methanol extract and protocatechuic acid reduced the activity of aniline hydroxylase that had been increased by bromobenzene, while did not affect the activities of aminopyrine N-demethylase and glutathione S-transferase. The methanol extract and compound effectively restored the activity of epoxide hydrolase which had been decreased by bromobenzene. These results may suggest that the methanol extract of Z. piperitum and protocatechuic acid prevented lipid peroxidation by reducing the activity of aniline hydroxylase, an epoxide-producing enzyme, and by enhancing the activity of epoxide hydrolase, an epoxide-removing enzyme, in rats that had been intoxicated with bromobenzene.

Schistosomicidal Evaluation of Zanthoxylum naranjillo and its Isolated Compounds against Schistosoma mansoni Adult Worms

“…1) were in agreement with previously published data, which allowed the identifi cation of protocatechuic acid (1), gallic acid (2), p-hydroxybenzoic acid (3), and 5-O-caffeoylshikimic acid (4) (Hur et al, 2003;Gohar et al, 2003;Pouchert, 1992;Veit et al, 1992). Previous phytochemical studies on the aerial parts of this plant have described the isolation of lignans and sesquiterpenoids (Bastos et al, 1996), but, to the best of our knowledge, this is the fi rst report of compounds 1 -4 in Z. naranjillo.…”

Aqueous Extracts of Dried Fruits of <i>Zanthoxylum armatum</i> DC., (Rutaceae) Induce Cellular and Nuclear Damage Coupled with Inhibition of Mitotic Activity <i>in Vivo</i>

“…Analysis of roots of Allium bulbs incubated in varying concentrations of AEDF exhibited a concentration dependent inhibition of a yellow coloured complex, previously reported to be an aldehyde-TBA complex [41,42] (result not shown). This is in agreement with previously published reports of the inhibition of lipid peroxidation by Zanthoxylum extracts [43,44] suggesting that the cel-lular damages induced by AEDF possibly does not involve induction of lipid peroxidation and might occur via an alternative mechanism(s).…”

Antifungal and cytotoxicity activities of Anacardium othonianum extract

“…The EF fraction was purified by chromatography, yielding four compounds, namely, amentoflavone (1), gallic acid (2), protocatechuic acid (3) and ethyl 3,4,5-trimethoxybenzoate (4) (Figure 1), as established by NMR spectra and comparisons with earlier recorded data (Markham et al, 1997;Gottlieb et al, 1991;Hur et al, 2003;Wu and Darcel, 2009). To the best of the authors' knowledge, this is the first report on compounds 1-4 in A. othonianum.…”