Effect of Methanol extract of <i>Musca domestica</i> larva on some Enzymes and Haematological parameters in <i>Trypanosoma brucei brucei</i> - infected rats

Abstract

This study investigated the effect of methanol extract of Musca domestica (400mg/kg body weight) on some biomarker enzymes and haematological parameters in Trypanosoma brucei brucei -infected rats. Twenty albino rats were intraperitoneally infected with Trypanosoma brucei brucei and were grouped into five (5) groups of four (4) rats each. Group1 was set up as infected not treated (0.2ml normal saline/kg body weight), group 2 was treated with diaminazene aceturate (standard drug), group 3 as prophylactic treated (treatment for 72 hours before inoculation of parasite), group 4 as early treatment with the extract (treatment commenced after the sight of parasite) and group 5 as the control (uninfected untreated) group. Results shows significant (p<0.05) decrease in liver AST and ALT activities with concomitant increase in serum activities of the infected untreated rats when compared with the early treated, prophylactic treated, standard treated and normal control. Serum ALP activity of the infected not treated group was significantly (p<0.05) higher when compared to the control group and other experimental groups. No significant (p>0.05) difference in the liver ALP activities of the extract treated infected groups with standard drug treated group However, serum and liver GGT activities of the uninfected untreated (control) was significantly lower (p<0.05) than all the other experimental groups. Haematological studies shows significant decrease (p<0.05) in packed cell volume (PCV) , haemoglobin concentration (Hb) and red blood cell count (RBC) of infected not treated when compared to infected prophylactic treated and infected early treated. There was likewise significant increase in white blood cell count (WBC) of infected not treated compared to infected prophylactic treated and infected early treated. Findings from this study showed that methanol extract of Musca domestica larva has trypanocidal properties thereby ameliorating the T. brucei induced biochemical changes in rats.

Trypanocidal activity of methanol extracts of the hemolymph of Sarcophaga argyrostoma larva against Trypanosoma evansi infected mice

“…This study demonstrates that 10 mg/ml concentrations of methanol extract of hemolymph of S. argyrostoma larva significantly inhibited T. evansi organisms in vivo. Similar results were recorded by Shittu et al [26] using methanol extract of M. domestica larva. The observed inhibition may be an indication of the possible utility of the methanol extract of hemolymph of larva as an anti-trypanosomal agent.…”

“…Similar results were recorded by Shittu et al . [ 26 ] using methanol extract of M. domestica larva. The observed inhibition may be an indication of the possible utility of the methanol extract of hemolymph of larva as an anti-trypanosomal agent.…”

“…Several studies have also previously reported significant increases in ALAT and ASAT activities in Trypanosoma sp. infected animals [26][27][28]. These studies explained that liver infection with Trypanosoma led to hepatocyte destruction and enzyme release and indicates a cellular response.…”

Anti-diabetic activities of Chromolaena odorata methanol root extract and its attenuation effect on diabetic induced hepatorenal impairments in rats

“…Hepatic impairment is one of the complications of diabetes mellitus and its evident by elevation of serum transaminase and alkaline phosphatases activities. Therefore, evaluation of serum enzymes biomarker will provide reliable indicator of functional integrity of the liver as well as treatment outcome [40][41][42] in diabetic condition. In the present study, the elevated levels of serum aspartate aminotransferase and alkaline phosphatase activities in diabetic untreated rats is an indication of plasma membrane and hepatic impairment, these will adversely hampered amino acid and carbohydrates metabolism and thus effect ATP production [43].…”

The prospects of the use of insects as an alternative source of proteins and medicine for human

“…Evaluation of Biochemical parameters has been proven to be a reliable method of assessing the deleterious effect of substance or outcome of a particular treatment on the integrity of organs that would have been compromised in stress or disease condition [14][15][16][17][18]. Insect and its products have been proven to provide significant protective effect to organs particularly the liver and kidney during toxicant/stress or parasite induced damages in animals [19]. Propolis from honey bee has been reported for haematopoietic properties [20] and hepatocurative effect against CCL4 toxicant [21].…”