Effect of Methanol‐Ammonia‐Water Treatment on the Concentration of Individual Glucosinolates of Canola

Shahidi, Fereidoon; Gabon, J.E.

doi:10.1111/j.1365-2621.1989.tb05979.x

Cited by 15 publications

(8 citation statements)

References 17 publications

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“…Samples were separated on a fused silica SPB-20 (30 m x 0.25 mm i.d.) capillary column, using the instrumentation and gas flow rates as described previously (Shahidi and Gabon, 1989). Injector and detector temperatures were both at 230°C.…”

Section: -Journalmentioning

confidence: 99%

Fate of Singrin in Methanol/Ammonia/Water‐Hexane Extraction of B. juncea Mustard Seed

Shahidi

Gabon

1990

Journal of Food Science

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The individual glucosinolates present in B. juncea mustard seed were determined. Sinigrin (ally1 glucosinolate) was the major glucosinolate of mustard seed and constituted 92.9% of the total glucosinolates. The effect of CHaOH/NHJH,O-hexane treatment on the concentration of glucosinolates was studied. Over 97% of sinigrin originally present in the seed was removed by the above solvent extraction system, mainly in the intact form. Breakdown products of sinigrin were quantified in the treated meal, in the extracted oil and in the polar phase (gums). Aglycon products were ally1 cyanide, ally1 isothiocyanate and 2,3-epithiopropane cyanide. Sugar breakdown products were glucose, thioglucose, thioglucose dimer and furfuryl alcohol. Desulfosinigrin was a minor decomposition product and might have acted as an intermediate in the decomposition of sinigrin.

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Section: -Journalmentioning

confidence: 99%

Fate of Singrin in Methanol/Ammonia/Water‐Hexane Extraction of B. juncea Mustard Seed

Shahidi

Gabon

1990

Journal of Food Science

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“…Published work on glucosinolate removal from plants has focused on using batch liquid solvent extraction. The majority of these have been either on a small scale for quantification purposes or aimed at removal from food and feed products without concern for glucosinolate degradation 11–17. Faudet et al 18 studied the effects of several parameters on solid–liquid extraction of rapeseed meal.…”

Section: Introductionmentioning

confidence: 99%

Glucoraphanin extraction from Cardaria draba: Part 1. Optimization of batch extraction

Powell

Hill

Juurlink

et al. 2005

J of Chemical Tech & Biotech

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Glucosinolates have historically been considered an anti-nutritional component of food and feed cereal crops. Large-scale protocols have been aimed at complete glucosinolate elimination from plants, rather than maximizing the recovery of any particular glucosinolate compound. Recently, glucoraphanin, an alkenyl glucosinolate, has been found to have nutritional value in terms of anti-carcinogenic behavior and hypertension relief. In this work, we report on the efficient capture of glucoraphanin from the noxious weed Cardaria draba. The effect of temperature, ethanol content in the aqueous solvent, initial solvent pH, solids loading, and contact time on both glucoraphanin and glucosinalbin recovery were examined. The optimal extraction conditions, evaluated using 0.11 dm 3 stirred baffled vessels, were found to be 20% aqueous ethanol solvent at 70• C and an initial pH value of 3, extracted at a solid to liquid ratio of 50 g dmover 20 mins. The recovery achieved with the baffled vessels was up to three times greater than the glucoraphanin yield obtained using standard analytical procedures that involved the use of 8.0 × 10 −3 dm 3 of hot, 80% ethanol solutions in test tubes at the same solvent loading. This corresponds to 30 mg g −1 of glucoraphanin recovered from the dried C draba leaves, versus only 10 mg g −1 using the analytical method.

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“…Previously, Shahidi and Gabon [24] had shown that the extraction of canola meals with CH 3 OH/NH 3 /H 2 O-hexane decreased the level of glucosinolates by up to 85%.…”

Section: Sinigrin Decompositionmentioning

confidence: 99%

Decomposition of sinigrin by methanol/ammonia/water treatment in model systems and mustard (Brassica nigra L.) seed meal

Pecháček

Velı́šek

Davı́dek

2000

Eur Food Res Technol

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Sinigrin (allylglucosinolate) was stored at 20 7C, 40 7C, and 60 7C in solutions containing 10% ammonia in 95% methanol (CH 3 OH/NH 3 /H 2 O). The individual samples were analyzed for their contents of sinigrin, its decomposition product allyl isothiocyanate (AITC) and other reaction products. The major reaction products were allyl thiourea, methyl ester of Nallyl thiocarbamoyl acid and allyl cyanide. A newly identified decomposition product of sinigrin was ethylcyanide. Defatted Brassica nigra seed meal was treated by the CH 3 OH/NH 3 /H 2 O mixture to simulate the procedure used for rape and mustard seed meal detoxification. Suspensions of the meal in the above mixture of solvents were heated at 20 7C, 40 7C and 60 7C, respectively. The major reaction products were the same as those arising from sinigrin. Minor products identified were 4-hydroxybenzyl isothiocyanate formed from sinalbin and 2-hydroxy-butenyl cyanide which arises from progoitrin. In all cases, AITC was very rapidly decomposed. Mechanisms explaining sinigrin decomposition are presented and discussed.

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Effect of Methanol‐Ammonia‐Water Treatment on the Concentration of Individual Glucosinolates of Canola

Cited by 15 publications

References 17 publications

Fate of Singrin in Methanol/Ammonia/Water‐Hexane Extraction of B. juncea Mustard Seed

Fate of Singrin in Methanol/Ammonia/Water‐Hexane Extraction of B. juncea Mustard Seed

Glucoraphanin extraction from Cardaria draba: Part 1. Optimization of batch extraction

Decomposition of sinigrin by methanol/ammonia/water treatment in model systems and mustard (Brassica nigra L.) seed meal

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