Effect of methacrylic acid beads on the sonic hedgehog signaling pathway and macrophage polarization in a subcutaneous injection mouse model

Lisovsky, Alexandra; Zhang, David; Sefton, Michael V.

doi:10.1016/j.biomaterials.2016.04.033

Cited by 42 publications

(35 citation statements)

References 77 publications

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“…Methacrylic acid (MAA)‐based materials are vascular regenerative materials that accelerate wound healing . They can be made in different forms, such as beads, porous scaffolds, hydrogels, and smooth coatings .…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies shed light on the mechanism of action of MAA. Macrophages interacting with this biomaterial are biased toward an anti‐inflammatory phenotype (M2) and have an increased secretion of insulin growth factor 1 (IGF‐1) . This secretion increases endothelial cell proliferation and migration consistent with the higher vessel densities in the surrounding tissue and improved healing.…”

Section: Introductionmentioning

confidence: 99%

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Methacrylic Acid Copolymer Coating Enhances Constructive Remodeling of Polypropylene Mesh by Increasing the Vascular Response

Coindre

Carleton

Sefton

2019

Adv Healthcare Materials

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This study reports that a methacrylic acid (MAA)‐based copolymer coating generates constructive remodeling of polypropylene (PP) surgical mesh in a subcutaneous model. This coating is non‐bioresorbable and follows the architecture of the mesh without impeding connective tissue integration. Following implantation, the tissue response is biased toward vascularization instead of fibrosis. The vessel density around the MAA mesh is double that of the uncoated mesh two weeks after implantation. This initial vasculature regresses after two weeks while mature vessels remain, suggesting an enhanced healing response. Concurrently, the MAA coating alters the foreign body response to the mesh. Fewer infiltrating cells, macrophages, and foreign body giant cells are found at the tissue–material interface three weeks after implantation. The coating also dampens inflammation, with lower expression levels of pro‐inflammatory and fibrogenic signals (e.g., Tgf‐β1, Tnf‐α, and Il1‐β) and similar expression levels of anti‐inflammatory cytokines (e.g., Il10 and Il6) compared to the uncoated mesh. Contrary to other coatings that aim to mitigate the foreign body response to PP mesh, a MAA coating does not require the addition of any biological agents to have an effect, making the coated mesh an attractive candidate for soft tissue repair.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Methacrylic Acid Copolymer Coating Enhances Constructive Remodeling of Polypropylene Mesh by Increasing the Vascular Response

Coindre

Carleton

Sefton

2019

Adv Healthcare Materials

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“…The expression of Shh in macrophages was also suggested in the present study (Supplementary information, Fig. 4B) and investigated separately [49]. The majority of cells expressing GFP and b-Gal in the surrounding healthy tissue were within hair follicles (Figs.…”

Section: Effect Of Maa Beads On Shh Signaling In Non-diabetic Woundsmentioning

confidence: 73%

“…2A). In a related study, the subcutaneous injection of MAA beads increased the vessel density (compared to MM beads) in both sexes at day 7; similarly, the response in females was more modest than in males [49]. It has been previously suggested that males and females differ in their inherent angiogenic potential [50,51].…”

Section: Sex Differences In Response To Maa Beadsmentioning

confidence: 79%

“…Here, the Shh pathway was modulated in multiple cell types both within granulation tissue and healthy skin. In vitro experiments with primary macrophages [49] and SKPs (data not shown) imply that direct activation of Shh by the biomaterial is possible. However, beads became entrapped within scabs and did not associate directly with the cells within the granulation tissue or in the skin surrounding the wound bed.…”

Section: Shh Signaling Pathway In Maa-mediated Vascularizationmentioning

confidence: 89%

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Shh pathway in wounds in non-diabetic Shh-Cre-eGFP/Ptch1-LacZ mice treated with MAA beads

Lisovsky

Sefton

2016

Biomaterials

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Unlocking Transplant Tolerance with Biomaterials

Pham,

Coronel

2024

Adv Healthcare Materials

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For patients suffering from organ failure due to injury or autoimmune disease, allogeneic organ transplantation with chronic immunosuppression is considered the gold standard in terms of clinical treatment. However, the true “holy grail” of transplant immunology is operational tolerance, in which the recipient exhibits a sustained lack of alloreactivity toward unencountered antigen presented by the donor graft. This outcome is resultant from critical changes to the phenotype and genotype of the immune repertoire predicated by the activation of specific signaling pathways responsive to soluble and mechanosensitive cues. Biomaterials have emerged as a medium for interfacing with and reprogramming these endogenous pathways towards tolerance in precise, minimally invasive, and spatiotemporally defined manners. By viewing seminal and contemporary breakthroughs in transplant tolerance induction through the lens of biomaterials‐mediated immunomodulation strategies – which include intrinsic material immunogenicity, the depot effect, graft coatings, induction and delivery of tolerogenic immune cells, biomimicry of tolerogenic immune cells, and in situ reprogramming – this review emphasizes the stunning diversity of approaches in the field and spotlights exciting future directions for research to come.This article is protected by copyright. All rights reserved

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Effect of methacrylic acid beads on the sonic hedgehog signaling pathway and macrophage polarization in a subcutaneous injection mouse model

Cited by 42 publications

References 77 publications

Methacrylic Acid Copolymer Coating Enhances Constructive Remodeling of Polypropylene Mesh by Increasing the Vascular Response

Methacrylic Acid Copolymer Coating Enhances Constructive Remodeling of Polypropylene Mesh by Increasing the Vascular Response

Shh pathway in wounds in non-diabetic Shh-Cre-eGFP/Ptch1-LacZ mice treated with MAA beads

Unlocking Transplant Tolerance with Biomaterials

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