Effect of menopause on hormonal receptors in ampullae of the fallopian tube with a special reference to the p53 signature

Abstract

ObjectivesAge-related changes in the expression of hormonal receptors have not been well examined in the fallopian tube (FT). We herein report the effect of menopause on the hormone receptors in ampullae of the FTs (AFTs), in comparison with cortical inclusion cysts (CICs) of the ovary.MethodsA total of 84 AFTs and 16 fimbriae of FTs, which were obtained from 26 premenopausal and 58 postmenopausal women; and 27 postmenopausal CICs were immunohistochemically studied for the expression of p53, Ki-67, estrogen receptor-alpha (ER-α), and progesterone receptor A (PRA). Apoptotic cells were identified using a TUNEL assay.ResultsPostmenopausal AFTs showed a significantly lower labeling index (LI) for Ki-67 (P<0.001), apoptosis (P=0.03), and PRA (P<0.001) than premenopausal AFTs. No significant correlation with immunohistochemical markers was found in premenopausal AFTs, but the LI for PRA was positively correlated with that for Ki-67 (P=0.004) and inversely with that for p53 (P=0.023) in postmenopausal AFTs. The expression of immunohistochemical markers was closely correlated between ampullae and fimbriae of the FT. The p53 signature (p53S) was detected in five postmenopausal AFTs (mean age: 70.2 years) and was not detected in any CICs. The immunohistochemical profile of p53S was low expression of Ki-67, apoptosis, and PRA, and high expression of ER-α. The expression of PRA in CICs was significantly higher than that in AFTs (P=0.001).ConclusionThe expression of PRA was significantly lower in postmenopausal AFTs than in premenopausal AFTs, whereas the expression of PRA was well preserved in postmenopausal CICs.

Estrogen and Progesterone Receptor Immunoexpression in Fallopian Tubes among Postmenopausal Women Based on Time since the Last Menstrual Period

“…This finding is consistent with other reports revealing ER and PR immunolocalization in fallopian tubes of both pre-and postmenopausal women. Expression of ER and PR was demonstrated in the nuclei of ciliated and nonciliated cells of the fallopian tubes, as well as in the tubal stroma and smooth muscle cells [17,18,[20][21][22]24,[31][32][33][34][35][36][37].…”

“…There are available reports on the immunolocalization and immunoexpression of ER and PR in the fallopian tubes of premenopausal women [17][18][19][20][21][22][23][24]. However, according to current knowledge, there are only a few reports on the expression of ERα and PR in normal human fallopian tubes after menopause [17][18][19][20].…”

“…There are available reports on the immunolocalization and immunoexpression of ER and PR in the fallopian tubes of premenopausal women [17][18][19][20][21][22][23][24]. However, according to current knowledge, there are only a few reports on the expression of ERα and PR in normal human fallopian tubes after menopause [17][18][19][20]. Some authors have revealed that in the fallopian tube of postmenopausal women, ERα and PR expression decreased or remained virtually undetectable, while other researchers have found that ERα and PR expression increased [17,18,20].…”

“…However, according to current knowledge, there are only a few reports on the expression of ERα and PR in normal human fallopian tubes after menopause [17][18][19][20]. Some authors have revealed that in the fallopian tube of postmenopausal women, ERα and PR expression decreased or remained virtually undetectable, while other researchers have found that ERα and PR expression increased [17,18,20]. In turn, other studies showed weak expression of ERα and strong expression of PR [19].…”

Morphofunctional Organization of the Uterine Tubes in the Conditions of Pathology

“…Початкові дослідження показали, що аденозин в низьких концентраціях викликає слабке тонічне скорочення м'язової оболонки маткових труб, а у високихпригнічення скорочень. У наступних дослідженнях показано, що ця реакція зменшується при запальному процесі у маткових трубах [63]. Серія експериментів присвячена вивченню впливу аденозину на циліарну активність війчастих клітин, а саме він збільшує частоту коливання війок [64].…”