Effect of membrane cholesterol on dimyristoylphosphatidylcholine-induced vesiculation of human red blood cells

Frenkel, Erik J.; Kuypers, Frans A.; Kamp, J.A.F. Op den; Roelofsen, B.; Ott, Peter

doi:10.1016/0005-2736(86)90177-x

Cited by 15 publications

(8 citation statements)

References 25 publications

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“…Among the component lipids, CH has well‐known effects on lipid bilayer rigidity and stability 21–26. Bilayer stability effects are reflected in the effect of CH concentration on phase transition temperature and saturated lipid segregation,27 bilayer fusion temperature,28 and bilayer fluidity 26.…”

Section: Discussionmentioning

confidence: 99%

Improving ultrasound reflectivity and stability of echogenic liposomal dispersions for use as targeted ultrasound contrast agents

Huang

Hamilton

Nagaraj

et al. 2001

Journal of Pharmaceutical Sciences

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Section: Discussionmentioning

confidence: 99%

Improving ultrasound reflectivity and stability of echogenic liposomal dispersions for use as targeted ultrasound contrast agents

Huang

Hamilton

Nagaraj

et al. 2001

Journal of Pharmaceutical Sciences

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“…Cholesterol Depletion-Cholesterol extraction from rabbit erythrocytes was performed using phosphatidylcholine liposomes (22,23). Rabbit erythrocytes (4 ϫ 10 8 cells/ml) were incubated with 1 mM phosphatidylcholine liposomes at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…Binding Site for ␣-Toxin on Rabbit Erythrocytes-Rabbit erythrocytes were suspended in buffer containing 15 mM dextran 4 to prevent hemolysis and depleted of cholesterol by incubation with liposomes (22,23). This method was employed because rapid depletion with methyl-␤-cyclodextrin led to high cellular fragility and spontaneous hemolysis.…”

Section: Cholesterol Depletion Selectively Destroys the High Affinitymentioning

confidence: 99%

Evidence That Clustered Phosphocholine Head Groups Serve as Sites for Binding and Assembly of an Oligomeric Protein Pore

Valeva

Hellmann

Walev

et al. 2006

Journal of Biological Chemistry

107

111

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High susceptibility of rabbit erythrocytes toward the poreforming action of staphylococcal ␣-toxin correlates with the presence of saturable, high affinity binding sites. All efforts to identify a protein or glycolipid receptor have failed, and the fact that liposomes composed solely of phosphatidylcholine are efficiently permeabilized adds to the enigma. A novel concept is advanced here to explain the puzzle. We propose that low affinity binding moieties can assume the role of high affinity binding sites due to their spatial arrangement in the membrane. Evidence is presented that phosphocholine head groups of sphingomyelin, clustered in sphingomyelin-cholesterol microdomains, serve this function for ␣-toxin. Clustering is required so that oligomerization, which is prerequisite for stable attachment of the toxin to the membrane, can efficiently occur. Outside these clusters, binding to phosphocholine is too transient for toxin monomers to find each other. The principle of membrane targeting in the absence of any genuine, high affinity receptor may also underlie the assembly of other lipid-inserted oligomers including cytotoxic peptides, protein toxins, and immune effector molecules.

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“…Spontaneous vesiculation in human erythrocytes has also been observed for a variety of triggering mechanisms. Limited examples of these include incubation with sonicated dimyristoylphosphatidylcholine vesicles (Frenkel et al, 1986;Butikofer et al, 1987), increased intracellular calcium (Allan and Thomas, 1981), ATP depletion (Lutz et al, 1977), and upon addition of a variety of amphiphiles (Hagerstrand and Isomaa, 1989) for exocytotic events, and with, for instance, treatment 'Addition of myelin basic protein was found to inhibit vesiculation in the case of myelin lipids (Fraser et al, 1986 of ghosts with ATP (Birchmeier et al, 1979) for endovesiculation. In general, the size of the vesicles produced in these experiments seems to be in the range 200 nm-1 ,um, which is too large to result in the isotropic lines seen in the experiments described here (below).…”

Section: Introductionmentioning

confidence: 99%

Temperature-reversible eruptions of vesicles in model membranes studied by NMR

Nezil¹,

Bayerl²,

Bloom³

1992

Biophysical Journal

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Deuterium (2H) and phosphorus (31P) nuclear magnetic resonance (NMR) and freeze-fracture electron microscopy were used to study spontaneous vesiculation in model membranes composed of POPC:POPS with or without cholesterol. The NMR spectra indicated the presence of a central isotropic line, the intensity of which is reversibly and linearly dependent upon temperature in the L alpha phase, with no hysteresis when cycling between higher and lower temperatures. Freeze-fracture microscopy showed small, apparently connected vesicles that were only present when the samples were frozen (for freeze-fracture) from an initial temperature of 40-60 degrees C, and absent when the samples are frozen from an initial temperature of 20 degrees C. Analysis of motional narrowing was consistent with the isotropic lines being due to lateral diffusion in (and tumbling of) small vesicles (diameters approximately 50 nm). These results were interpreted in terms of current theories of shape fluctuations in large unilamellar vesicles which predict that small daughter vesicles may spontaneously "erupt" from larger parent vesicles in order to expel the excess area created by thermal expansion of the bilayer surface at constant volume. Assuming that all the increased area due to increasing temperature is associated with the isotropic lines, the NMR results allowed a novel estimate of the coefficient of area expansion alpha A in multilamellar vesicles (MLVs) which is in good agreement with micromechanical measurements upon giant unilamellar vesicles of similar composition. Experiments performed on unilamellar vesicles, which had been placed upon glass beads, confirmed that alpha A determined in this way is unchanged compared with the MLV case. Addition of the highly positively charged (extrinsic) myelin basic protein (MBP) to a POPC:POPS system showed that membrane eruptions of the type described here occur in response to the presence of this protein.

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Effect of membrane cholesterol on dimyristoylphosphatidylcholine-induced vesiculation of human red blood cells

Cited by 15 publications

References 25 publications

Improving ultrasound reflectivity and stability of echogenic liposomal dispersions for use as targeted ultrasound contrast agents

Improving ultrasound reflectivity and stability of echogenic liposomal dispersions for use as targeted ultrasound contrast agents

Evidence That Clustered Phosphocholine Head Groups Serve as Sites for Binding and Assembly of an Oligomeric Protein Pore

Temperature-reversible eruptions of vesicles in model membranes studied by NMR

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