Effect of mechanical stress on differentiation of mouse mesenchymal stem cells seeded into an octacalcium phosphate–gelatin scaffold

Yamada, Masakazu; Anada, Takahisa; Masuda, Taisuke; Takano‐Yamamoto, Teruko; Сузукі, Осаму

doi:10.1016/j.snb.2015.05.073

Cited by 4 publications

(2 citation statements)

References 47 publications

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“…25 In fact, the restoration of bone formation by the alleviation of mechanical stress was observed with the aid of a polytetrafluoroethylene (PTFE) ring around the materials. 32 These results suggest that the osteogenic cellular activities around OCP were surely controlled not only by the materials activity but also by the given environment around the materials. 32 These results suggest that the osteogenic cellular activities around OCP were surely controlled not only by the materials activity but also by the given environment around the materials.…”

Section: Introductionmentioning

confidence: 90%

Osteogenic cellular activity around onlaid octacalcium phosphate‐gelatin composite onto rat calvaria

Iwama

Anada

Tsuchiya

et al. 2018

J Biomedical Materials Res

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The activity and the distribution of osteogenic cells around octacalcium phosphate (OCP) granules combined with gelatin matrix (OCP/Gel) onlaid on rat calvaria were analyzed histomorphometrically and immunohistochemically during vertical bone augmentation under mechanical or nonmechanical environment until 8 weeks. OCP/Gel disk was placed in subperiosteal pocket on the calvaria with or without polytetrafluoroethylene (PTFE) support. The latter is a nonmechanical stress model to alleviate the mechanical stress from the subcutaneous tissues. Onlay grafts of gelatin (Gel) sponge disk and OCP granules were also carried out for the comparison purpose. When bone augmentation was evaluated in first area from bone surface (area until 150 μm high from bone surface) and second area above the newly formed bone (area until 150 µm high from the first area), bone formation was enhanced most in first area followed by second area of OCP/Gel with PTFE. The appearance of tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like cells was suppressed more in the newly formed tissue with PTFE than those without PTFE with an emphasis of the presence of gelatin. Although Runx2 positive-cells were accumulated more in both OCP/Gel with and without PTFE, osteocalcin-positive cells were abundant in OCP/Gel with PTFE than that without PTFE, suggesting that nonmechanical stress condition is more suitable for osteoblast differentiation. The appearance of receptor activator of nuclear factor (NF)-kappaB ligand (RANKL)-positive cells was restrained in OCP/Gel and Gel with PTFE while osteoprotegerin-positive cells were most accumulated in OCP/Gel without PTFE. The results suggest that OCP/Gel composite under the mechanical stress-alleviated condition can enhance bone augmentation through balanced osteoblastic and osteoclastic cellular activities. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1322-1333, 2018.

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Section: Introductionmentioning

confidence: 90%

Osteogenic cellular activity around onlaid octacalcium phosphate‐gelatin composite onto rat calvaria

Iwama

Anada

Tsuchiya

et al. 2018

J Biomedical Materials Res

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“…Compared with 2D environment, MSCs loaded on 3D scaffolds are studied more extensively in recent years, which is more closely to the physiological conditions in vivo 2 . It was found that compressive stress could promote osteogenic differentiation of MSCs in octacalcium phosphate-gelatin scaffold under a certain stress amplitude (20%) 143 . However, excessive stress amplitude (40%, 60%) inhibited the differentiation of MSCs.…”

Section: Biomaterials-induced Physicomechanical Stimuli Towards Mscsmentioning

confidence: 99%

Integrating physicomechanical and biological strategies for BTE: biomaterials-induced osteogenic differentiation of MSCs

Shi¹,

Zhou²,

Wang³

et al. 2023

Theranostics

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Large bone defects are a major global health concern. Bone tissue engineering (BTE) is the most promising alternative to avoid the drawbacks of autograft and allograft bone. Nevertheless, how to precisely control stem cell osteogenic differentiation has been a long-standing puzzle. Compared with biochemical cues, physicomechanical stimuli have been widely studied for their biosafety and stability. The mechanical properties of various biomaterials (polymers, bioceramics, metal and alloys) become the main source of physicomechanical stimuli. By altering the stiffness, viscoelasticity, and topography of materials, mechanical stimuli with different strengths transmit into precise signals that mediate osteogenic differentiation. In addition, externally mechanical forces also play a critical role in promoting osteogenesis, such as compression stress, tensile stress, fluid shear stress and vibration, etc. When exposed to mechanical forces, mesenchymal stem cells (MSCs) differentiate into osteogenic lineages by sensing mechanical stimuli through mechanical sensors, including integrin and focal adhesions (FAs), cytoskeleton, primary cilium, ions channels, gap junction, and activating osteogenic-related mechanotransduction pathways, such as yes associated proteins (YAP)/TAZ, MAPK, Rho-GTPases, Wnt/β-catenin, TGFβ superfamily, Notch signaling. This review summarizes various biomaterials that transmit mechanical signals, physicomechanical stimuli that directly regulate MSCs differentiation, and the mechanical transduction mechanisms of MSCs. This review provides a deep and broad understanding of mechanical transduction mechanisms and discusses the challenges that remained in clinical translocation as well as the outlook for the future improvements.

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Bone Repairment via Mechanosensation of Piezo1 Using Wearable Pulsed Triboelectric Nanogenerator

Wang

Zhu

et al. 2022

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Bone repair in real time is a challenging medical issue for elderly patients; this is mainly because aged bone marrow mesenchymal stem cells (BMSCs) possess limited osteogenesis potential and repair capacity. In this study, triboelectric stimulation technology is used to achieve bone repair via mechanosensation of Piezo1 by fabricating a wearable pulsed triboelectric nanogenerator (WP‐TENG) driven by human body movement. A peak value of 30 µA has the optimal effects to rejuvenate aged BMSCs, enhance their osteogenic differentiation, and promote human umbilical vein endothelial cell tube formation. Further, previous studies demonstrate that triboelectric stimulation of a WP‐TENG can reinforce osteogenesis of BMSCs and promote the angiogenesis of human umbilical vein endothelial cells (HUVECs). Mechanistically, aged BMSCs are rejuvenated by triboelectric stimulation via the mechanosensitive ion channel Piezo1. Thus, the osteogenesis potential of BMSCs is enhanced and the tube formation capacity of HUVECs is improved, which is further confirmed by augmented bone repair and regeneration in in vivo investigations. This study provides a potential signal transduction mechanism for rejuvenating aged BMSCs and a theoretical basis for bone regeneration using triboelectric stimulation generated by a WP‐TENG.

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Effect of mechanical stress on differentiation of mouse mesenchymal stem cells seeded into an octacalcium phosphate–gelatin scaffold

Cited by 4 publications

References 47 publications

Osteogenic cellular activity around onlaid octacalcium phosphate‐gelatin composite onto rat calvaria

Osteogenic cellular activity around onlaid octacalcium phosphate‐gelatin composite onto rat calvaria

Integrating physicomechanical and biological strategies for BTE: biomaterials-induced osteogenic differentiation of MSCs

Bone Repairment via Mechanosensation of Piezo1 Using Wearable Pulsed Triboelectric Nanogenerator

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