Bone Repairment via Mechanosensation of Piezo1 Using Wearable Pulsed Triboelectric Nanogenerator

Abstract

Bone repair in real time is a challenging medical issue for elderly patients; this is mainly because aged bone marrow mesenchymal stem cells (BMSCs) possess limited osteogenesis potential and repair capacity. In this study, triboelectric stimulation technology is used to achieve bone repair via mechanosensation of Piezo1 by fabricating a wearable pulsed triboelectric nanogenerator (WP‐TENG) driven by human body movement. A peak value of 30 µA has the optimal effects to rejuvenate aged BMSCs, enhance their osteogenic differentiation, and promote human umbilical vein endothelial cell tube formation. Further, previous studies demonstrate that triboelectric stimulation of a WP‐TENG can reinforce osteogenesis of BMSCs and promote the angiogenesis of human umbilical vein endothelial cells (HUVECs). Mechanistically, aged BMSCs are rejuvenated by triboelectric stimulation via the mechanosensitive ion channel Piezo1. Thus, the osteogenesis potential of BMSCs is enhanced and the tube formation capacity of HUVECs is improved, which is further confirmed by augmented bone repair and regeneration in in vivo investigations. This study provides a potential signal transduction mechanism for rejuvenating aged BMSCs and a theoretical basis for bone regeneration using triboelectric stimulation generated by a WP‐TENG.

PFKFB3-mediated glycometabolism reprogramming modulates endothelial differentiation and angiogenic capacity of placenta-derived mesenchymal stem cells

“…S347), they have harvested human bone marrow specimens from the ilia of healthy volunteer donors. Isolation and identification of human BMMSCs had been conducted as they had previously reported [31,32]. BMMSCs were cultured in DMEM containing 15% FBS and 1% penicillin-streptomycin.…”

Applications of Triboelectric Nanogenerators in Bone Tissue Engineering

“…As anticipated, this wearable pulsed TENG could generate up to 30 µA current to provide the optimal effect for aged BMSCs. [ 26 ] This electrical stimulation could enhance osteogenic differentiation and proliferation of aged BMSCs, and associated angiogenesis and tube formation of human umbilical vein endothelial cells (Figure 6c). In the animal study, the team implanted aged BMSCs with/without electrical stimulation into the cranial defect site.…”

Adaptive Triboelectric Nanogenerators for Long-Term Self-Treatment: A Review

“…Due to the limited osteogenic capacity of bone marrow mesenchymal stem cells (BMSCs) in elderly patients, treating elderly patients in need of bone repair has always been a challenging medical problem. Here, Wang et al performed bone repair by means of the mechanical action of body−driven wearable triboelectric nanogenerators (WP-TENG) and piezoelectric ceramics ( Figure 9 a) [ 71 ]. To improve the friction performance of the equipment, nylon sheets were selected as the base material; soft foam was attached to the nylon to increase the friction, polytetrafluoroethylene (PTFE) film and aluminum foil were used as positive and negative friction layers, and two copper foils were attached to the back of the two friction layers as electrodes.…”

“… ( a ) Human limb movement activates the WP−TENG to achieve bone repair. Reprinted with permission from [ 71 ]. 2022, John Wiley and Sons.…”