Effect of matrix metalloproteinase inhibitors on microtensile bond strength of dental composite restorations to dentin in use of an etch‐and‐rinse adhesive system

Abstract

Aim This study assesses the effect of matrix metalloproteinases on microtensile bond strength (μTBS) of an etch‐and‐rinse adhesive system. Methods This in vitro study evaluated 88 extracted premolars. The teeth were sectioned to expose dentin and were then randomly divided into four groups (n = 22). In group 1 (control), dentin surface was etched, and Adper Single Bond 2 was applied. In groups 2–4, dentin surface was etched and chlorhexidine (CHX), 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide (EDC), and dimethyl sulfoxide (DMSO) were applied on the surfaces, respectively, and blotted dry. Next, Adper Single Bond 2 was applied and all teeth were built up with Z350 composite. In each group, half the samples immediately and the other half after 10,000 thermal cycles underwent μTBS test. Data were analyzed using ANOVA and Tukey's test (α = .05). Results In thermocycled samples, maximum μTBS was noted in CHX group followed by DMSO, EDC, and control group (p < .001). The thermocycled μTBS of composite to dentin was significantly higher in CHX group compared with EDC, DMSO, and control groups (p < .001) but was not significantly different in EDC and DMSO groups (p = .498). Conclusion The thermocycled μTBS obtained by the application of CHX, EDC, and DMSO was significantly higher compared with the value to the control group.

The effect of immediate dentin sealing with chlorhexidine pretreatment on the shear bond strength of dual‐cure adhesive cement

“…The CHX pretreatment group seemed to be able to ensure the highest penetration of adhesive into the collagen network and eventuated higher bond strength (Saffarpour et al, 2020;Zheng & Chen, 2017).…”

“…Due to these unprotected collagen fibrils, the hybrid layer is hydrolyzed, and bond strength decreases. The durability of dentin‐resin bonding could be improved by the application of MMP inhibitors (Saffarpour, Valizadeh, Amini, Kharazifard, & Rohaninasab, 2020; Shen et al, 2020). Micromechanical interlocking is enhanced by the reactions between MMP substrate and inhibitors that enable the conjunction of MMP inhibitors and dental adhesive systems (Frassetto, Breschi, Turco, et al, 2016; Saffarpour et al, 2020).…”

“…The durability of dentin‐resin bonding could be improved by the application of MMP inhibitors (Saffarpour, Valizadeh, Amini, Kharazifard, & Rohaninasab, 2020; Shen et al, 2020). Micromechanical interlocking is enhanced by the reactions between MMP substrate and inhibitors that enable the conjunction of MMP inhibitors and dental adhesive systems (Frassetto, Breschi, Turco, et al, 2016; Saffarpour et al, 2020). It is clear that to make the adhesive interface more durable, any technique that preserves the collagen fibers and restrains the movement of fluid inside the hybrid layer will be effective (Pashley, Tay, Yiu, et al, 2004).…”

“…Furthermore, within universal adhesive groups, CHX pretreatment caused much more cohesive‐in‐dentin failure types and increased the number of resin tags in SEM observations. The CHX pretreatment group seemed to be able to ensure the highest penetration of adhesive into the collagen network and eventuated higher bond strength (Saffarpour et al, 2020; Zheng & Chen, 2017). This finding could be defined by the fact that CHX might potentially be chemically inconsistent with adhesives and could interact with collagen fibrils exposed by acid etching (da Rosa et al, 2018).…”

Effect of pretreatment with matrix metalloproteinase inhibitors on the durability of bond strength of fiber posts to radicular dentin

“…It has been proposed that activation of carboxylic groups and cross‐linking of collagen fibrils by EDC preserve the dentin–resin bond mechanical properties (Liu et al, 2011; Tezvergil‐Mutluay et al, 2012). Various EDC solutions have been investigated to find a protocol of EDC dentin surface coating that best improves the bond strength over time; however, little attention has been paid to radicular dentin (Mazzoni, Angeloni et al, 2013; Mazzoni et al, 2018; Saffarpour et al, 2020; Tang et al, 2016).…”