Effect of marine oil and rapessee oil on composition of fatty acids in lipoprotein triacylglycerols from rat blood plasma and liver perfusate

Thomassen, Magny S.; Strom, E. V.; Christiansen, Erling N.; Norum, Kaare R.

doi:10.1007/bf02533567

Cited by 50 publications

(21 citation statements)

References 29 publications

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“…Christiansen et al [7] fed PHMO and peanut oil to rats for 3 weeks and found by incubation a delayed uptake of 22:1 in the hepatocytes compared to 16:0, but an increased oxidation of 22:1 in the PHMO group, relative to the peanut oil group. However, as this and other studies [2][3][4][6][7][8] were based on diets deficient in linoleic acid (18:2 n-6), these results may not be fully comparable with those from well-nourished, high-fat-adapted rats. The overall metabolism of high fat diets rich in 22:1 acids needs further elucidation.…”

Section: Introductioncontrasting

confidence: 44%

“…How ever, in the present paper, and in accordance with other reports [27,28,34] appreciable amounts of labelled TG (40-50% of the total radioactivity in the medium) were secreted from the hepatocytes, presumably as VLDL-TG. Thomassen et al [8] suggested that most of the dietary 22:1 reaching the liver is se creted as VLDL-TG or chain-shortened. They also found evidence for an existence of two different TG pools in the liver; one for storage and one for secretion.…”

Section: Discussionmentioning

confidence: 99%

“…Due to a better commercial availability of labelled erucic acid this fatty acid has been used in studying the in vitro metabolism of 22:1 acids. In this regard in terest has been focused on the adaptive mechanism through an increased liver per oxisomal P-oxidation of the erucic acid in rats fed PHMO [6,7], leading to chainshortened fatty acids, predominantly 18:1, incorporated into liver phospholipids and triglycerides (TG) [7] as well as into VLDL-TG [7,8].…”

Section: Introductionmentioning

confidence: 99%

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Comparative Metabolism of Erucic and Oleic Acid in Hepatocytes from Rats Fed Partially Hydrogenated Marine Oil or Palm Oil

1987

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Rats were fed for 8 weeks high fat diets containing 20% of either palm oil (PO) or linoleic-supplemented partially hydrogenated marine oil (PHMO). Hepatocytes were isolated from rats fasted overnight. Incubations were performed with albumin bound [10–¹⁴C] oleic acid and [14–¹⁴C] erucic acid, respectively, for 15 or 60 min. The cell viability was better than 90% after 60 min of incubation. The incorporation of radioactive metabolites into lipids of the cells and of the secreted products was determined. The uptake and channelling of 18:1 and 22:1 into various lipid classes in the hepatocytes were different, but were little influenced by the diets. Erucic acid disappeared more rapidly from the incubation medium than 18:1 at both incubation times and for both dietary groups, and was mainly incorporated into the hepatocyte triglycerides and found in the free fatty acid pool, whereas only small amounts of labelling were detected in the phospholipids. Oleic acid, however, was readily incorporated into both phospholipids and triglycerides, and little was found in the free fatty acid pool. For both the triglycerides and the free fatty acids more than 90% of the radioactivity was represented by the incubation fatty acid. Chain-shortened products were found in the phospholipids, especially after incubation with 22:1. The chain-shortening capacity was somewhat higher in the PHMO group, relative to the PO group. At both incubation times, the major part (80–90%) of the total radioactivity was found in the lipid-soluble fraction of the incubation medium, and for both groups 60–80% still as the original incubation fatty acid. 20–30% of the radioactivity was found in the triglycerides secreted from the cells. Phospholipids were secreted in minor amounts (1–5%). Water soluble products constituted 3–7% at 15 min of incubation, increasing to 10–12% at 60 min, highest when incubating with 22:1 and similar for both dietary groups.

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Section: Introductioncontrasting

confidence: 44%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Metabolism of Erucic and Oleic Acid in Hepatocytes from Rats Fed Partially Hydrogenated Marine Oil or Palm Oil

1987

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“…The proton density of methylene groups in myocardial tissue triglycerides can vary from one individual to another and ideally would be measured separately for each subject. Given the risks associated with cardiac biopsy we used average value estimated for animal myocardium (12, 21). 2) Myocardial tissue water content is 73% by weight (22).…”

Section: Methodsmentioning

confidence: 99%

Myocardial triglycerides and systolic function in humans: In vivo evaluation by localized proton spectroscopy and cardiac imaging

Szczepaniak

Dobbins

Metzger

et al. 2003

Magnetic Resonance in Med

403

325

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“…Extracted plasma lipids and lipids from feed samples were methylated (Appelqvist 1968; Hoshi et al. 1973; Thomassen et al. 1979) prior to gas chromatography analysis (CP9001, Chrompack, Middelburg, the Netherlands) using a CP9050 auto sampler to inject samples (1 μL).…”

Section: Methodsmentioning

confidence: 99%

The effect of hepatic passage on postprandial plasma lipid profile of rainbow trout (Oncorhynchus mykiss) after a single meal

Eliason

Djordjevic

Trattner

et al. 2010

Aquaculture Nutrition

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For the first time, pre‐ and post‐hepatic plasma lipid profiles were monitored following a single meal in a free‐swimming, non‐anaesthetized fish. Rainbow trout (Oncorhynchus mykiss; 700–1500 g; 10 °C) were equipped with cannulae in the dorsal aorta (DA) and hepatic portal vein (HPV). Simultaneous blood samples, taken from both cannulae at 0, 3, 6, 12, 24 and 48 h postprandial, revealed the time course of the plasma lipid profiles following a single meal (1% of body mass). Primarily monounsaturated fatty acids with the exception of 18:1n − 9, increased significantly from baseline by 12 h postprandial without greatly affecting total plasma lipid concentrations. Total plasma lipids then showed a small peak at 24 h postprandial, coinciding with a peak in triacylglycerols. We conclude that assimilation of lipids from the digest into the plasma is slower than reported for proteins and carbohydrates in the same species. Furthermore, as there were no significant differences between the HPV and DA, no measurable effect of hepatic passage on plasma lipid levels was resolved. Therefore, we also conclude that, in contrast to that in higher vertebrates, hepatic passage does not seem to have a major role in rainbow trout for modulating the postprandial plasma profile of lipids.

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Effect of marine oil and rapessee oil on composition of fatty acids in lipoprotein triacylglycerols from rat blood plasma and liver perfusate

Cited by 50 publications

References 29 publications

Comparative Metabolism of Erucic and Oleic Acid in Hepatocytes from Rats Fed Partially Hydrogenated Marine Oil or Palm Oil

Comparative Metabolism of Erucic and Oleic Acid in Hepatocytes from Rats Fed Partially Hydrogenated Marine Oil or Palm Oil

Myocardial triglycerides and systolic function in humans: In vivo evaluation by localized proton spectroscopy and cardiac imaging

The effect of hepatic passage on postprandial plasma lipid profile of rainbow trout (Oncorhynchus mykiss) after a single meal

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