Effect of mango seed kernel extract on the adipogenesis in 3T3-L1 adipocytes and in rats fed a high fat diet

Abstract

Mangoes (Mangifera indica L.) are one of the most important tropical foods. The seed is one of the main by-products of mango processing. Therefore, it is important to find an economically viable use for this waste (e.g., as a food additive or supplement with high nutraceutical value). We investigated the anti-obesity effects of mango seed kernel extract with hot water (MSKE-W) in 3T3-L1 adipocytes and in a high fat diet (HFD)-induced obesity rat model. MSKE-W caused a significant decrease in the activity of glycerol 2-phosphate dehydrogenase in 3T3-L1 adipocytes without eliciting cell cytotoxicity and inhibited cellular lipid accumulation through down-regulation of transcription factors such as PPARγ and C/EBPα. In the animal model, rats fed an HFD containing 1% MSKE-W gained less weight than rats fed an HFD alone. The visceral fat mass in rats fed an HFD containing 1% MSKE-W tended to be lower than that in rats fed an HFD alone. Furthermore, histological examination of rat livers from an HFD showed steatohepatitis. However, rats on an HFD containning 1% MSKE-W showed no histopathological changes in liver tissue. Our results indicate that MSKE-W influences anti-obesity effects, both in vitro and in vivo, and suggest that MSKE-W provides a novel preventive potential against obesity.

Nutritional and Pharmacological Properties of Agro-Industrial by-Products From Commonly Consumed Fruits

“…The inhibition of Igf-1 and its receptor at the early process is the precise step for the therapy of obesity [155]. Distillate aqueous extract of rambutan peel declined the level of triglycerides, size of the adipocyte, mRNA level of FABP4 gene and PPARγ expression on obesity rat model [156].…”

Natural dipeptidyl peptidase-IV inhibitor mangiferin mitigates diabetes- and metabolic syndrome-induced changes in experimental rats

“…It has been demonstrated that in a high-fat diet (HFD)-treated mice, mango seed kernel extract prevented weight gain and liver steatohepatitis. 11 Moreover, M. indica extract downregulated the expression of several genes associated with the pathophysiology of obesity and inflammation, such as lipoprotein lipase, hormone-sensitive lipase, fatty acid synthase, and resistin present in liver and epididymal fat. 12 Although several benefits of MNG have been reported by multiple pathways, there is no experimental evidence presently available in the literature with regard to its beneficial effect on metabolic syndrome coexisting with diabetes mellitus.…”

“…MNG has been shown to counteract obesity/metabolic syndrome. It has been demonstrated that in a high-fat diet (HFD)-treated mice, mango seed kernel extract prevented weight gain and liver steatohepatitis 11. Moreover, M. indica extract downregulated the expression of several genes associated with the pathophysiology of obesity and inflammation, such as lipoprotein lipase, hormone-sensitive lipase, fatty acid synthase, and resistin present in liver and epididymal fat 12.…”