Effect of mammary secretions on functions of polymorphonuclear leukocytes in pigs

Österlundh, Ingrid; Holst, H.; Magnusson, Ulf

doi:10.2460/ajvr.2001.62.1250

Cited by 5 publications

(2 citation statements)

References 26 publications

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“…bovine total leukocyte microarray; neutrophil biology; apoptosis; inflammation MAMMALS UNDERGO VARYING DEGREES of immunosuppression and disease susceptibility during late pregnancy and parturition (8,15,17,27). Leukocytes from dairy cows provide an excellent model for studying parturient immune suppression, as these cells exhibit impaired inflammatory responses that associate with leukocytosis and increased susceptibility of the animals to opportunistic bacteria, such as gram-negative coliforms that cause mastitis in infected mammary glands (12,15).…”

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Microarray analysis of gene expression in blood neutrophils of parturient cows

Madsen

Chang

Hickey

et al. 2004

Physiological Genomics

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It is well documented that blood neutrophils from parturient dairy cows do not perform as well as neutrophils from nonparturient cows in laboratory assays of adhesion, migration, or phagocytosis-induced respiratory burst. However, little is known about the possible molecular basis for parturition-induced changes in neutrophils. cDNA microarray analysis was used in the current study to explore parturition-induced changes in gene expression profiles in bovine blood neutrophils. Total RNA from isolated blood neutrophils of four parturient Holstein cows was obtained before, during, and after parturition, reverse transcribed into cDNA, and sequentially labeled with Cy3 or Cy5 dyes prior to paired hybridizations to 1,056 member bovine total leukocyte (BOTL-3) microarrays in a loop design. Resulting gene expression data were LOWESS normalized by array and analyzed using a mixed model approach. Results showed that expression profiles for 302 BOTL-3 genes were influenced by parturition. BLASTn analysis and preliminary clustering of affected genes by biological function indicated that the largest proportion (14%) of changed genes encode proteins critical to regulation of apoptosis. Independent confirmation of altered expression for 16 of these genes was achieved using quantitative real-time RT-PCR (Q-RT-PCR). A predominantly survival phenotype inferred from the microarray and Q-RT-PCR results was substantiated by monitoring apoptosis status of blood neutrophils from castrated male cattle cultured in the presence of sera from parturient cows. Thus our combined gene expression and apoptosis phenotyping results suggest that bovine parturition may induce prolonged survival in normally short-lived blood neutrophils.

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Microarray analysis of gene expression in blood neutrophils of parturient cows

Madsen

Chang

Hickey

et al. 2004

Physiological Genomics

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“…To combat a microbial infection, neutrophils circulating in blood migrate by endothelium towards a chemoattractant gradient to the site of infection, then phagocyte and destroy the pathogen using some killing mechanisms including antimicrobial enzymes such as elastase, myeloperoxidase (MPO), and alkaline phosphatase (ALKP) (2,3,15). Elastase, as well as MPO are toxic mediators in a neutrophil arsenal for killing bacterial pathogens, additionally, MPO activity is often used as an index of neutrophil content and activation (2).…”

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Elastase, Myeloperoxidase, and Alkaline Phosphatase Release and Free Radical Generation in Neutrophils Isolated from Blood of Sows at Different Stages of Oestrous Cycle

Wessely-Szponder

Bobowiec

2013

Bulletin of the Veterinary Institute in Pulawy

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The aim of the study was to assess the influence of the oestrous cycle phase on neutrophil secretory activity and to extrapolate it to susceptibility to uterine infections. The obtained results indicate that the highest enzyme release seen in the late follicular phase (elastase release was 42.18 ±3.11% of maximal release, myeloperoxidase was 45.0 ±5.12%, and alkaline phosphatase was 44.75 ±9.0%) was related to the level of 17β-oestradiol in plasma. Similarly, a free radical generation was also the most enhanced during this phase. Significantly lower values were obtained from sows during the luteal phase in regard to both enzyme release (36.62 ±3.58% for elastase, 27.87 ±8.7% for myeloperoxidase, and 22.12 ±2.4% for alkaline phosphatase), and that of free radicals (2.28 1.6 μM/10 6 cells for nitric oxide and 2.47 0.6 nM/10 6 cells for superoxide).

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