Effect of low-level laser therapy on odontoblast-like cells exposed to bleaching agent

Lima, Adriano Fonseca; Ribeiro, Ana Paula Dias; Basso, Fernanda Gonçalves; Bagnato, Vanderlei Salvador; Hebling, Josimeri; Marchi, Giselle Maria; Costa, Carlos Alberto de Souza

doi:10.1007/s10103-013-1309-2

Cited by 19 publications

(26 citation statements)

References 40 publications

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“…Dental bleaching has been widely studied, with most of the research related to side effects, such as toxic effects on the pulp cells, [1][2][3][4] deleterious effects on dental pulp, 5,6 decreases in microhardness of the bleached substrate, [7][8][9][10] and the deleterious effect of bleaching on the bond strength of resin materials. [11][12][13][14] One of the theories regarding the deleterious effect of bleaching on the bond strength of resin materials is related to the decrease in bond strength with the reactive oxygen species (ROS) release by the bleaching agents and the accumulation of these ROSs on the bleached tooth structure.…”

Section: Introductionmentioning

confidence: 99%

Effect of Tooth Bleaching on Bond Strength of Enamel-Dentin Cavities Restored With Silorane- and Dimethacrylate-based Materials

Lima

Sasaki²,

Araújo³

et al. 2011

Operative Dentistry

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Clinical RelevancePrior bleaching treatment, with both low and high concentrations of bleaching agents, does not affect the push-out bond strength of dimethacrylate-and silorane-based materials to enamel-dentin cavities. SUMMARYThe aim of this study was to evaluate the influence of tooth bleaching on the push-out bond strength of a composite resin based on dimethacrylates and silorane to cavities that involve both enamel and dentin. A total of 80 bovine incisors were sectioned on the buccal surface to obtain specimens (10 3 10 mm) presenting enamel and dentin (1-mm thick . After treatment, cavities were made (1.2-mm diameter on dentin; 1.5-mm diameter on enamel) with a diamond bur. At 24 hours after restoration, a push-out bond strength test was performed at a crosshead speed of 0.5 mm/min. The bleaching treatments did not significantly affect the bond strengths of either restorative system to enamel-dentin. Regardless of the bleaching treatment, the dimethacrylate-based resin system exhibited significantly higher bond strengths to enamel-dentin than did the silorane-based system.

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Section: Introductionmentioning

confidence: 99%

Effect of Tooth Bleaching on Bond Strength of Enamel-Dentin Cavities Restored With Silorane- and Dimethacrylate-based Materials

Lima

Sasaki²,

Araújo³

et al. 2011

Operative Dentistry

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“…However, as shown in a previous study carried by Lima et al . , no modulation of cell viability was observed. In addition, the authors demonstrated that the cell metabolism of cultured odontoblasts exposed to bleaching agents was not affected by laser irradiation, except at the energy dose of 15 J cm −2 , at which cell metabolism was decreased.…”

Section: Discussionmentioning

confidence: 86%

Effects of Laser Irradiation on Pulp Cells Exposed to Bleaching Agents

Lima

Basso

Ribeiro

et al. 2013

Photochem & Photobiology

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The aim of this study was to evaluate the effect of low-level laser therapy (LLLT) on odontoblast-like cells exposed to a bleaching agent. Mouse dental papilla cell-23 cells were seeded in wells of 24-well plates. Eight groups were established according to the exposure to the bleaching agent and LLLT (0, 4, 10 and 15 J cm À2). Enamel-dentin disks were adapted to artificial pulp chambers, which were individually placed in wells containing Dulbecco's modified Eagle's medium (DMEM). A bleaching agent (35% hydrogen peroxide [BA35%HP]) was applied on enamel (15 min) to obtain the extracts (DMEM + BA35%HP components diffused through enamel/dentin disks). The extracts were applied (1 h) to the cells, and then subjected to LLLT. Cell viability (Methyl tetrazolium assay), alkaline phosphatase (ALP) activity, as well as gene expression of ALP, fibronectin (FN) and type I collagen, were evaluated. The bleaching procedures reduced the cell viability, ALP activity and gene expression of dentin proteins. Laser irradiation did not modulate the cell response; except for FN, as LLLT decreased the gene expression of this protein by the cells exposed to the BA35%HP. It can be concluded that BA35%HP decreased the activities of odontoblasts that were not recovered by the irradiation of the damaged cells with low-level laser parameters tested.

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“…Also, SA is capable of protecting the pulp cells against the toxic effects of sub-products released from the bleaching agent [42]. When the antioxidant was applied before bleaching, less damage to the odontoblastlike cells MDPC-23 was found, suggesting the SA solution may protect against the deleterious effect of hydrogen peroxide [43]. Others studies have reported that SA can also promote resistance to fracture of teeth after internal bleaching [44] reduce microleakage [45,46,47], and restored the decrease in the Ca/P ratio after bleaching [48].…”

Section: Effects Of Sodium Ascorbate In Other Dental Propertiesmentioning

confidence: 99%

O ascorbato de sódio promove a resistência de união após técnicas de clareamento dental?

Nascimento

Guerreiro

Carvalho

et al. 2016

Rev. Odonto Ciênc. (Online)

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Objective: To summarize and discuss the available information concerning the effectiveness of sodium ascorbate in improving bond strength after dental bleaching procedures. Methods: Information of original articles or reviews listed in PubMed database using searching terms: ("sodium ascorbate" OR antioxidant) AND (bleaching OR hydrogen peroxide OR carbamide peroxide), were included in the review. Results: Most of studies revealed that sodium ascorbate could restore the altered redox potential of the oxidized bonding substrate, thus reversing the compromised bonding. However, several protocols of this antioxidant have been used in combination with different peroxide concentrations and exposure time to bleaching agents. Conclusion: Studies should be conducted to establish an appropriate application protocol and to evaluate the effect of sodium ascorbate in other dental properties.

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Effect of low-level laser therapy on odontoblast-like cells exposed to bleaching agent

Cited by 19 publications

References 40 publications

Effect of Tooth Bleaching on Bond Strength of Enamel-Dentin Cavities Restored With Silorane- and Dimethacrylate-based Materials

Effect of Tooth Bleaching on Bond Strength of Enamel-Dentin Cavities Restored With Silorane- and Dimethacrylate-based Materials

Effects of Laser Irradiation on Pulp Cells Exposed to Bleaching Agents

O ascorbato de sódio promove a resistência de união após técnicas de clareamento dental?

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