Effect of low-level laser therapy (808 nm) on skeletal muscle after endurance exercise training in rats

Abstract

| Background: Low-level laser therapy (LLLT) has been demonstrated to be effective in optimizing skeletal muscle performance in animal experiments and in clinical trials. However, little is known about the effects of LLLT on muscle recovery after endurance training. Objective: This study evaluates the effects of low-level laser therapy (LLLT) applied after an endurance training protocol on biochemical markers and morphology of skeletal muscle in rats. Method: Wistar rats were divided into control group (CG), trained group (TG), and trained and laser irradiated group (TLG). The endurance training was performed on a treadmill, 1 h/day, 5 days/wk, for 8 wk at 60% of the maximal speed reached during the maximal effort test (Tmax) and laser irradiation was applied after training. Results: Both trained groups showed significant increase in speed compared to the CG. The TLG demonstrated a significantly reduced lactate level, increased tibialis anterior (TA) fiber cross-section area, and decreased TA fiber density. Myogenin expression was higher in soleus and TA muscles in both trained groups. In addition, LLLT produced myogenin downregulation in the TA muscle of trained animals. Conclusion: These results suggest that LLLT could be an effective therapeutic approach for stimulating recovery during an endurance exercise protocol.Keywords: low-level laser therapy; endurance exercise; lactate; skeletal muscle; myogenin; physical therapy. BULLET POINTS• LLLT applied after an endurance training protocol.• LLLT decreased lactate concentration at rest.• LLLT improved muscle fiber morphology.• LLLT decreased myogenin expression.• LLLT could be an effective therapeutic approach for stimulating recovery. HOW TO CITE THIS ARTICLEAssis L, Yamashita F, Magri AMP, Fernandes KR, Yamauchi L, Renno ACM. Effect of low-level laser therapy (808 nm) on skeletal muscle after endurance exercise training in rats. Braz J Phys Ther. 2015 Nov-Dec; 19(6):457-465. http://dx

Strength training prior to muscle injury potentiates low-level laser therapy (LLLT)-induced muscle regeneration

“…5). LLLT has been demonstrating efficiency not only improving and accelerating the skeletal muscle regeneration [1,15,17] but also providing positive influence on physical performance and muscle recovery [19,20,37,38]. In this sense, studies have been designed to evaluate the effects of combined therapies (LLLT and exercise practice) applied post muscleinduced injuries in skeletal muscle regenerative process [22,39].…”

“…In this sense, studies have been designed to evaluate the effects of combined therapies (LLLT and exercise practice) applied post muscleinduced injuries in skeletal muscle regenerative process [22,39]. Assis et al [19,39] demonstrated that LLLT and aerobic exercise association applied post a muscle-induced atrophy model significantly reduced muscle RING-finger protein-1 and Atrogin-1 mRNA levels, preventing muscle atrophy.…”

“…Further to improving skeletal muscle regeneration, a growing body of studies has been demonstrating positive effects of LLLT applied prior and post exercise sessions related to muscle recovery [18][19][20][21]. Further, a previous study evaluated the effect of chronic aerobic exercise practice and LLLT interaction applied post a musculoskeletal atrophy model, verifying that the interaction with exercise and LLLT was effective in preventing the muscle atrophy [22].…”

Histological analysis of the association of low level laser therapy and platelet-rich plasma in regeneration of muscle injury in rats

“…LLLT promotes angiogenesis and has anti-inflammatory properties, 26 , 27 , 28 while PRP contains Platelet-derived growth factor (PDGF), Transforming growth factor (TGF-β1), and Vascular endothelial growth factor (VEGF), responsible for attracting cells to the injury site and promoting tissue regeneration. 29 , 30 These factors probably reduced the damage in cells adjacent to the injury site, resulting in a lower incidence of fibers affected by the injury.…”

Vázizomsérülés Kezelések Jelenlegi Módjainak Áttekintése

“…Az eredmények azt mutatták, hogy a hő sérülés utáni azonnali alkalmazása felgyorsította a sérült hely degenerációs folyamatát, megkönnyítette a makrofágok vándorlását, az SC-k proliferációját és differenciálódását, valamint elősegítette az izomszövet regenerációját. Az alacsony intenzitású lézerterápiát (LLLT) szintén terápiás lehetőségként tartják számon az izmok javulásának és helyreállításának stimulálására patkányokon végzett állóképességi edzések eredményeinek alapján [ASSIS et al, 2015]. Az LLLT kombinálható vérlemezkékben gazdag vérplazma adásával, ami jobb eredményeket hozott az izmok regenerációjának elősegítésében sérülések után, mint az LLLT önmagában.…”