Effect of Low- Level Laser Therapy on Bone Regeneration of Critical-Size Bone Defects: A Systematic Review of In Vivo Studies and Meta-Analysis

Kheiri, Aida; Amid, Reza; Kheiri, Lida; Namdari, Mahshid; Mojahedi, Masoud; Kadkhodazadeh, Mahdi

doi:10.1016/j.archoralbio.2020.104782

Cited by 8 publications

(4 citation statements)

References 19 publications

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“…This fact can be considered as one of the main gaps to be filled in the coming years. This theory is confirmed by recent systematic reviews that analyzed the effects of PBM therapy on bone repair, even though these studies included studies involving the use of biomaterials, systemic conditions or even bone grafts 45,46 .…”

Section: Bone Repair and Photobiomodulationmentioning

confidence: 73%

Effects of Photobiomodulation on Experimental Bone Repair in Animal models: a Systematic Review

Neto¹,

Lilge²,

Damante³

et al. 2021

JMS

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Introduction: photobiomodulation was showed positive effects on bone healing, however, several studies search safe protocols and dosimetry, beyond good experimental models. Thus, the present study aimed to conduct a systematic review of photobiomodulation (PBM) effects on bone healing increase, focusing on animal experimental models. Revision: the studies search on the PBM use in bone repair was carried out in the PubMed / MEDLINE and Lilacs databases, excluding studies that included systemic conditions and biomaterials or medications use. Sixteen studies were selected, within the inclusion and exclusion criteria stipulated. Animal models included rats (n = 14) and rabbits (n = 2). The laser parameters applied ranging between 650 nm and 830 nm and the power density ranging from 0.03 W to 6 W. Animal models were mainly concentrated in rodents, and the most utilized defect type was the tibial bone defect with 2.5 mm of diameter. Conclusion: through this review we concluded that the location and size of the defects, as well as the laser irradiation parameters are diverse and have shown the lack of consensus on the topic, however the results for using PBM therapy are encouraging.

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Section: Bone Repair and Photobiomodulationmentioning

confidence: 73%

Effects of Photobiomodulation on Experimental Bone Repair in Animal models: a Systematic Review

Neto¹,

Lilge²,

Damante³

et al. 2021

JMS

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“…The LLLT parameters used in our study (6 J/cm 2 , singledose, and 650 nm) differ from other studies in the cell target, laser equipment, culture conditions (well plates, spot diameter), and cell concentration. 28 Therefore, we considered it a key to the establishment of the irradiation parameters and the cell type to determine the best aspects for stimulation. Our study showed the LLLT positive effects on SCAPs proliferation.…”

Section: Discussionmentioning

confidence: 99%

Low-Level Laser Irradiation Promotes Proliferation and Differentiation on Apical Papilla Stem Cells

et al. 2021

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Introduction: Low-level laser therapy (LLLT) has been reported to improve cell proliferation and differentiation. The stem cells derived from dental apical papilla (SCAPs) are a promising therapy because they are easily obtained from immature human teeth. The effect of LLLT over SCAPs is still unknown. This study aimed to evaluate the proliferation and osteogenic potential of the SCAPs stimulated with LLLT. Methods: SCAPs were isolated from the third molars of a healthy donor and characterized according to the minimum established criteria. SCAPs were cultured for 24 hours before being exposed to LLLT. Cells were exposed to different doses, energy, and wavelengths for selecting the irradiation parameters. SCAPs proliferation was evaluated with the MTT assay at 24 hours and 7-day post-laser exposure. VEGF and TGFβ2 expression were assessed with a specific enzyme-linked immunosorbent assay (ELISA). The osteogenic differentiation potential was analyzed with alizarin red staining, and the nodule quantification was performed by the relative optical density (ROD) analysis using ImageJ software. Results: The cells isolated from the apical papilla showed phenotype and stem cell properties. SCAPs irradiated with one dose at 6 J/m2 and 650 nm exhibited significantly higher proliferation (P>0.05) than the controls nonirradiated. LLLT stimulated SCAPs’ expression of factors VEGF and TGFβ2. Also, SCAPs irradiated showed higher osteogenic activity (P<0.05). Conclusion: LLLT promotes proliferation, osteogenic differentiation, and VEGF and TGFβ2 expression on SCAPs. LLLT is a practical approach for the preconditioning of SCAPs in vitro for future regenerative therapies. More studies are needed to determine the underlying molecular processes that determine the mechanism of the LLLT.

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“…Conceptually, they are defined as those in which there is no spontaneous regeneration of the surgical site, completely throughout the animal's life (Schimidt & Hollinger, 1986), without any intervention. In these cases, tissue filling is consolidated by the deposition of fibrous connective tissue (Spicer et al, 2012;Miguel et al, 2013;Lappalainen et al, 2015;Horváthy et al, 2016;Kheiri et al, 2020). Therefore, the regeneration of these losses and defects, as well as the aesthetic and functional restoration in these conditions, remains a major challenge in clinical approaches.…”

Section: Introductionmentioning

confidence: 99%

Hydroxyapatite, alginate and gelatin composites used for bone regeneration: a systematic review

Santos

Santos²,

Miguel³

et al. 2023

RSD

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Aim: to investigate and describe, through a systematic review, the biological behavior and osteogenic potential of composite biomaterials containing hydroxyapatite (HA), alginate and gelatin, in different associations, after in vivo implantation. Materials and Methods: for the search and selection of articles, the Medical Literature Analysis and Retrieval System Online (PubMed/MEDLINE) and Scientific Electronic Library Online (SciELO) databases were used, published between 2012 and 2022, using the descriptors: “bone regeneration”; “biocompatible material”; “durapatite”; “alginate”; “gelatin”. Initially, an association was made with the Boolean operator "OR" between the descriptors and their respective entry terms, considering that the MeSH and DeCS platforms use different terms to refer to the same keywords. Subsequently, the "AND" operator was used in nine associations between the descriptors. Results: during the searches, 1939 articles were located. After using the inclusion and exclusion criteria, 16 studies were included in the review. The main themes found in the searches were: HA and Alginate; HA and Gelatin; HA, Alginate and Zinc; HA, Gelatin and mesenchymal cells; HA, Alginate and Chitosan; HA, Alginate and Silk Fibrin; HA, Gelatin and titanium dioxide; HA, Alginate and Gelatin. It was observed that HA, when associated with alginate or gelatin, has improved its osteogenic properties. Final Considerations: HA composites associated with alginate and gelatin provide a range of applications and promising strategies applied to bone repair. Studies have shown that these composites have great potential for application in Bone Tissue Bioengineering.

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Effect of Low- Level Laser Therapy on Bone Regeneration of Critical-Size Bone Defects: A Systematic Review of In Vivo Studies and Meta-Analysis

Cited by 8 publications

References 19 publications

Effects of Photobiomodulation on Experimental Bone Repair in Animal models: a Systematic Review

Effects of Photobiomodulation on Experimental Bone Repair in Animal models: a Systematic Review

Low-Level Laser Irradiation Promotes Proliferation and Differentiation on Apical Papilla Stem Cells

Hydroxyapatite, alginate and gelatin composites used for bone regeneration: a systematic review

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