Effect of Low‐Dose Doxycycline on Serum Oxidative Status, Gingival Antioxidant Levels, and Alveolar Bone Loss in Experimental Periodontitis in Rats

“…Several studies reported the role of antioxidant enzymes, including glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD), in periodontitis in humans 9,10,13–15 and rats. 16 …”

mentioning

confidence: 99%

Periodontitis in Rats Induces Systemic Oxidative Stress That Is Controlled by Bone‐Targeted Antiresorptives

Oktay

Chukkapalli

Rivera‐Kweh

et al. 2015

Journal of Periodontology

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Background Periodontitis is a chronic, polymicrobial inflammatory disease that degrades connective tissue and alveolar bone and results in tooth loss. Oxidative stress has been linked to the onset of periodontal tissue breakdown and systemic inflammation, and the success of antiresorptive treatments will rely on how effectively they can ameliorate periodontal disease–induced oxidative stress during oral infection. Methods Rats were infected with polybacterial inoculum consisting of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia, as an oral lavage every other week for 12 weeks. Daily subcutaneous injections of enoxacin, bisenoxacin, alendronate, or doxycycline were administered for 6 weeks after 6 weeks of polybacterial infection in rats. The serum levels of oxidative stress parameters and antioxidant enzymes, including glutathione peroxidase, superoxide dismutase, and catalase, were evaluated in each of the infected, treated, and sham-infected rats. Results Rats infected with the periodontal pathogens displayed a five-fold increase in the oxidative stress index compared with controls as a result of increased levels of serum oxidants and decreases in total antioxidant activity. The overall decrease in antioxidant activity occurred despite increases in three important antioxidant enzymes, suggesting an imbalance between antioxidant macromolecules/small molecules production and antioxidant enzyme levels. Surprisingly, the bone-targeted antiresorptives bis-enoxacin and alendronate inhibited increases in oxidative stress caused by periodontitis. Bis-enoxacin, which has both antiresorptive and antibiotic activities, was more effective than alendronate, which acts only as an antiresorptive. Conclusion To the best of the authors’ knowledge, this is the first study to demonstrate that the increased oxidative stress induced by periodontal infection in rats can be ameliorated by bone-targeted antiresorptives.

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“…Fourteen days later, left ischemia and reperfusion were performed. At the end of the experiment, it was concluded that glutamine improves levels of total antioxidant capacity 23,24 . In the results of the described study, it was observed that glutamine showed the potential to avoid bone progression through supplementation of the drug.…”

Section: Discussionmentioning

confidence: 98%

Effect of glutamine ingestion on the progression of induced periodontitis: experimental study in rats

Silva

Segundo

Semenoff

et al. 2018

Rev. odontol. UNESP

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ResumoIntrodução: Com a suplementação de glutamina há um melhor desempenho no sistema de defesa do corpo. Objetivo: O objetivo deste estudo foi analisar o efeito da suplementação de glutamina na doença periodontal induzida por ligatura em ratos. Material e método: Foram selecionados 48 ratos machos da linhagem Wistar divididos em 4 grupos (N = 12): grupo controle (GC) incluindo animais saudaveis recebendo solução salina diariamente via gavagem; grupo glutamina (GG) incluindo animais saudaveis que receberam suplementação oral de glutamina por gavagem a uma dose de 1,5 g/kg/dia; grupo periodontite (GP) incluindo animais com doença periodontal induzida na maxila em ambos os lados recebendo solução salina diária via gavagem; grupo experimental (GE) incluindo animais com doença periodontal induzida na maxila nos dois lados recebendo glutamina diária via gavagem na dose de 1,5 g/kg/dia. No 30º dia, todos os animais foram eutanasiados por excesso de anestésico. Descritores: Suplementos nutricionais; doenças periodontais; produtos com ação antimicrobiana. AbstractIntroduction: With glutamine supplementation there is better performance in the body's defense system. Objective: The aim of this study was to analyze the effect of glutamine supplementation on ligand-induced periodontal disease in rats. Material and method: 48 selected male Wistar rats were divided into 4 groups (N = 12): control group (CG) including healthy animals receiving daily saline solution via gavage; glutamine group (GG) including healthy animals receiving oral glutamine supplementation by gavage at a dose of 1.5 g / kg / day; periodontitis group (PG) including animals with induced periodontal disease on both upper sides receiving daily saline solution via gavage; experimental group (EG) including animals with induced periodontal disease on both upper sides receiving daily glutamine via gavage at a dose of 1.5 g / kg / day. On the 30th day, all animals were euthanized by excess anesthetic.

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Effect of Low‐Dose Doxycycline on Serum Oxidative Status, Gingival Antioxidant Levels, and Alveolar Bone Loss in Experimental Periodontitis in Rats

Abstract: Doxycycline helps to prevent periodontal tissue breakdown by inhibiting local and systemic oxidative stress.

Cited by 49 publications

References 80 publications

Antioxidant enzymes in periodontitis

Antioxidant enzymes in periodontitis

Periodontitis in Rats Induces Systemic Oxidative Stress That Is Controlled by Bone‐Targeted Antiresorptives

Effect of glutamine ingestion on the progression of induced periodontitis: experimental study in rats

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