Effect of low above-zero temperature on the content of low-molecular antioxidants and activities of antioxidant enzymes in green barley leaves

Radyuk, M. S.; Domanskaya, I. N.; Shcherbakov, R. A.; Шалыго, Н. В.

doi:10.1134/s1021443709020058

Cited by 39 publications

(22 citation statements)

References 21 publications

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“…A similar tendency was found in cold-stressed barley seedlings (Radyuk et al, 2009). Significant increments of CAT activity were obtained with E1 and E3 OG SEC fractions, which could be helpful to the plant antioxidant enzymatic system when dealing with unfavorable environmental conditions.…”

Section: Discussionsupporting

confidence: 75%

“…"Petit Havana" plants (5°C for 4 day) showed an unaltered SOD activity level (Gechev et al, 2003). Increments in SOD activity levels were found in cold-stressed barley seedlings (2°C for 1 day) (Radyuk et al, 2009). Contrary to these contrasting results, under our experimental conditions, the low SOD activity level in cold-stressed zucchini cotyledons was reverted by application of OG mixtures, suggesting that the antioxidant system in cold-stressed zucchini cotyledons could be reinforced.…”

Section: Discussionmentioning

confidence: 94%

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Oligoglucans as Elicitors of an Enzymatic Antioxidant System in Zucchini Squash (<i>Cucurbita pepo</i> L.) Seedlings at Low Temperature

Cerón‐García

González‐Aguilar

Vargas‐Arispuro

et al. 2011

American Journal of Agricultural and Biological Sciences

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Problem statement: Non-controlled low temperatures affect physiological and biochemical processes leading to Reactive Oxygen Species (ROS) accumulation in plants, which normally are stabilized by the antioxidant system. Superoxide Dismutase (SOD), Ascorbate Peroxidase (APX) and Catalase (CAT) are the most important ROS-detoxification enzymes in stressed plants. Approach: The activity of these enzymes were examined in response to fungal Oligoglucans (OG) in cold-stressed cotyledons of Cucurbita pepo L. Cold stress condition was settled in zucchini cotyledons using relative Ion Leakage (IL) and H 2 O 2 accumulation. Then, OG were obtained from Trichoderma harzianum cellwall by enzymatic and chemical hydrolysis. These elicitors were purified by size-exclusion and anionexchange chromatography and tested in cold-stressed cotyledons. Results: No significant changes on H 2 O 2 content and IL were observed between treated and control cold-stressed cotyledons, during the first 7 days of storage at 5°C. Afterwards H 2 O 2 values increased greatly after cold-stressed. Then, cold stress conditions were established (7 d, 5°C). The APX and CAT activities remained stable, but not SOD activity, which decreased about 42% in control cotyledons. When a chemical OG mixture (CM) was applied to cold-stressed cotyledons, APX and CAT activity levels increased. The SOD activity was only increased by an enzymatic OG mixture (EM). The APX activity level was increased in coldstressed cotyledons using OG size-exclusion chromatography fractions from both CM and EM; however, changes in CAT activity were only possible using EM fractions and no changes were detected in SOD activity. The highest CAT activity levels were triggered using OG anion-exchange chromatography sub-fractions from both CM and EM fractions (120 and 119 % of enzymatic activity, respectively). Conclusion: The oxidative stress can be counteracted by the triggering of APX and CAT activity in cold-stressed zucchini cotyledons mediated by T. harzianum cell-wall OG.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 94%

Oligoglucans as Elicitors of an Enzymatic Antioxidant System in Zucchini Squash (<i>Cucurbita pepo</i> L.) Seedlings at Low Temperature

Cerón‐García

González‐Aguilar

Vargas‐Arispuro

et al. 2011

American Journal of Agricultural and Biological Sciences

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“…When the stress increased, GSH concentrations dropped and redox state became more oxidized, which marked the degradation of the system [135]. Similar to drought stress, altered ratio of GSH/GSSG has been observed in plants under various stresses like salinity [136], chilling [126], and metal toxicity [11,[13][14][15]. Overexpression of enzyme glutathione synthetase involved in GSH biosynthesis showed no effect on GSH level and was not sufficient to improve ozone tolerance [137] and resistance to photoinhibition [138] in hybrid poplar (Populus tremula × P. alba).…”

Section: Glutathionementioning

confidence: 96%

“…AsA level has been reported to alter in response to various stresses [8,11,13,14,125,126]. The level of AsA under environmental stresses depends on the balance between the rates and capacity of AsA biosynthesis and turnover related to antioxidant demand [127].…”

Section: Nonenzymatic Components Of Antioxidative Defense Systemmentioning

confidence: 99%

“…Nonenzymic antioxidants (AsA, GSH, carotenoids, and α-tocopherol) also play important role in cold response. Under cold stress conditions, low-molecular weight antioxidants, especially, that of reduced AsA, have been suggested to be an important component in plant cell defense [126]. Many comparative studies using chillingtolerant and sensitive genotypes have shown greater antioxidant capacity in chilling-tolerant species compared to sensitive ones [253][254][255].…”

Section: Chillingmentioning

confidence: 99%

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Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions

et al. 2012

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Reactive oxygen species (ROS) are produced as a normal product of plant cellular metabolism. Various environmental stresses lead to excessive production of ROS causing progressive oxidative damage and ultimately cell death. Despite their destructive activity, they are well-described second messengers in a variety of cellular processes, including conferment of tolerance to various environmental stresses. Whether ROS would serve as signaling molecules or could cause oxidative damage to the tissues depends on the delicate equilibrium between ROS production, and their scavenging. Efficient scavenging of ROS produced during various environmental stresses requires the action of several nonenzymatic as well as enzymatic antioxidants present in the tissues. In this paper, we describe the generation, sites of production and role of ROS as messenger molecules as well as inducers of oxidative damage. Further, the antioxidative defense mechanisms operating in the cells for scavenging of ROS overproduced under various stressful conditions of the environment have been discussed in detail.

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Reactive Oxygen Species

Choudhary

Chaudhary

Agrawal

et al. 2017

Reactive Oxygen Species in Plants

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Effect of low above-zero temperature on the content of low-molecular antioxidants and activities of antioxidant enzymes in green barley leaves

Cited by 39 publications

References 21 publications

Oligoglucans as Elicitors of an Enzymatic Antioxidant System in Zucchini Squash (<i>Cucurbita pepo</i> L.) Seedlings at Low Temperature

Oligoglucans as Elicitors of an Enzymatic Antioxidant System in Zucchini Squash (<i>Cucurbita pepo</i> L.) Seedlings at Low Temperature

Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions

Reactive Oxygen Species

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