Effect of Local Nasal Immunotherapy on Nasal Blockage in Pollen-Induced Allergic Rhinitis of Guinea Pigs

Abstract

Local nasal immunotherapy may be clinically useful for allergic nasal blockage associated with nasal hyperresponsiveness. The mechanisms responsible for this effectiveness might not be related to IgE production. Additionally, the effectiveness for nasal tissue was dissociated from that seen for the ocular tissue.

Establishment and characterization of an experimental mouse model of allergic rhinitis

“…However, there is no standardized method that can be followed universally. For example, the time point and duration for performing IP or IN varied from each other, not to mention the dosage of allergen they utilized [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16]. Besides, most methods used to sensitize the airway for assessment of hyperresponsiveness to antigen presentation were intended to establish an animal model of asthma and seldom for a model of AR.…”

“…Among them, mice are the most common animals used [2][3][4][5][6][7], followed by guinea pig [8][9][10][11][12]. Operating antigen, which is also called ''allergen'', is important to sensitize mice and render them ''allergic''.…”

“…In addition to mice, guinea pigs [8][9][10][11][12] or brown Norway rats [15,22] have also been used. Mice provide an easy means of assessing allergic symptoms such as sneezing or nasal rubbing (represents the motion due to itching), while rats may be a better choice if nasal obstruction is the target response for measure.…”

“…For local sensitization, OVA was almost given via IN at various concentrations [2-8, 14, 21]. In addition to OVA, pollen [9][10][11][12][13] or even fungus (Aspergillus fumigatus) [16] can be an alternative choice of sensitizing allergen. Nabe et al applied one kind of Japanese cedar pollen (Cryptomeria japonica) for both general and local sensitization and elicited satisfactory responses [9][10][11][12][13].…”

“…Operating antigen, which is also called ''allergen'', is important to sensitize mice and render them ''allergic''. Ovalbumin (OVA) and pollen [9][10][11][12][13] are the universal reagents for experimental allergen use. After selecting the proper allergen, the most important step is to apply it into the mouse body to sensitize and elicit symptoms of AR.…”