Effect of local hIL-10 gene therapy on experimental periodontitis in ovariectomized rats

Li, Yanfen; Ma, Souzhi; Guo, Jianbin; Jiang, Jun; Luo, Kai; Yan, Fuhua; Xiao, Yin

doi:10.1080/00016357.2017.1292427

Cited by 5 publications

(4 citation statements)

References 37 publications

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“…Considering that MMPs activation depends on several cytokines, including IL‐1α and IL‐1β, 46,47 it is conceivable to suggest that the significant reduction in the IL‐1β immunoexpression caused by diacerein treatment may explain, at least in part, the lower MMP‐8 immunoexpression in the PDG. In addition, the gradual increase in the birefringent collagen content detected in the gingival mucosa of PDG supports this hypothesis since this cytokine exerts an important role in the activation of MMP‐8 in the inflamed periodontal tissues 48 and in the crevicular fluid 9 . Our findings showed a parallelism between IL‐1β and MMP‐8, suggesting that this cytokine may mediate the activity of enzymes, including MMP‐8, responsible for breakdown of periodontal tissues.…”

Section: Discussionsupporting

confidence: 80%

Diacerein‐induced interleukin‐1β deficiency reduces the inflammatory infiltrate and immunoexpression of matrix metalloproteinase‐8 in periodontitis in rat molars

Silva

Sasso‐Cerri

Cerri

2022

Journal of Periodontology

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Background Periodontitis is an immunoinflammatory disease that involves the release of cytokines and enzymes, including interleukin‐1 (IL‐1) and matrix metalloproteinases (MMPs). Diacerein is an anti‐IL‐1 drug used for the treatment of osteoarthritis. The aim of the study was to evaluate whether diacerein suppresses the inflammatory reaction and reduces the collagen degradation in the gingival connective tissue in periodontitis. Methods Fifty‐four male rats were distributed into three groups (n = 18 animals/group): 1) periodontitis + diacerein group (PDG), 2) periodontitis + saline group (PSG) and control Group (CG; without treatment). Periodontitis was induced for 7 days in the upper right first molars; after 7 days, the animals of PDG received 100 mg/kg of diacerein while in PSG, the animals received saline solution for 7, 15, and 30 days. The animals were killed and fragments of maxilla containing the right molars were processed for paraffin embedding. In hematoxlin & eosin‐stained sections, the volume density of inflammatory cells (VvIC) and fibroblasts (VvFb) in the gingival mucosa, the distances between the junctional epithelium (JE) to the cemento‐enamel junction (CEJ) and the CEJ to the alveolar process crest (AP) were obtained. The number of IL‐1β‒ and MMP‐8‒immunolabeled cells, and collagen content in the gingival mucosa were computed. Data were subjected to two‐way analysis of variance and Tukey post‐test (P ≤ 0.05). Results The PDG and PSG rats showed a significant increase in the distances of JE‐CEJ and CEJ‐AP. In all periods, the VvIC, the number of IL‐1β‒ and MMP‐8‒immunolabeled cells was significantly lower in PDG than in PSG while the collagen content was significantly greater in PDG than PSG. At 30 days, significant differences in the IL‐1β immunoexpression, collagen content, and in the MMP‐8 immunostaining were not seen between PDG and CG groups. Conclusions Our results show an inhibitory effect of diacerein on IL‐1β in the inflamed gingival mucosa of rat molars, decreasing the inflammatory infiltrate and immunoexpression of MMP‐8, and restoring the collagen content.

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Section: Discussionsupporting

confidence: 80%

Diacerein‐induced interleukin‐1β deficiency reduces the inflammatory infiltrate and immunoexpression of matrix metalloproteinase‐8 in periodontitis in rat molars

Silva

Sasso‐Cerri

Cerri

2022

Journal of Periodontology

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“…This result is concordant with previous evidence associating ancestral allele-carriers (C) for rs6667202 with decreased risk of aggressive periodontitis (OR = 0.77, 95% CI = 0.6–0.95, P = 0.016) in a German/Austrian population [ 28 ]. IL10 is a key regulatory cytokine involved in the suppression of inflammation and return to homeostatic state [ 80 ] and extensive evidence links increased levels of IL10 with resistance to inflammatory bone loss in experimental periodontitis [ 81 , 82 ]. However, in addition to actively suppressing inflammatory mechanisms, IL10 can interfere in some antimicrobial responses, such as T h 1-type responses that are involved in the control of periodontopathogens [ 83 , 84 ].…”

Section: Discussionmentioning

confidence: 99%

Genetic Association with Subgingival Bacterial Colonization in Chronic Periodontitis

Cavalla

Biguetti

Melchiades

et al. 2018

Genes

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Chronic periodontitis is the most prevalent form of inflammatory destructive bone disease and has been affecting humans since antiquity. Evidence suggest that genetic factors can highly influence periodontitis risk, modulating disease elements such as the susceptibility to microbial colonization and the nature of subsequent host-microbe interaction. Several single-nucleotide polymorphisms (SNPs) have been associated with the occurrence of periodontitis, but the full range of genetic influence in periodontitis outcomes remains to be determined. In this context, this study comprises an analysis of possible correlation between periodontitis-related genetic variants with changes in the subgingival microbiological pattern performed in a Brazilian population (n = 167, comprising 76 chronic periodontitis patients and 91 healthy subjects). For the genetic characterization, 19 candidate SNPs were selected based on the top hits of previous large genome wide association studies (GWAS), while the subgingival microbiota was characterized for the presence and relative quantity of 40 bacterial species by DNA-DNA checkerboard. The case/control association test did not demonstrate a significant effect of the target SNPs with the disease phenotype. The polymorphism rs2521634 proved significantly associated with Tannerella forsythia, Actinomyces gerencseriae, Fusobacterium periodonticum, and Prevotella nigrescens; rs10010758 and rs6667202 were associated with increased counts of Porphyromonas gingivalis; and rs10043775 proved significantly associated with decreased counts of Prevotella intermedia. In conclusion, we present strong evidence supporting a direct connection between the host’s genetic profile, specifically rs2521634, rs10010758, rs6667202, and rs10043775 polymorphisms, and the occurrence of chronic periodontitis-associated bacteria.

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“…229,[240][241][242][243] Liposome formulations were programmed to release the payload by external stimuli as sound waves or alter the immune microenvironment for the betterment of the inflamed tissue. 239,[244][245][246] In addition, to take advantage of naturally occurring carotenoid molecules such as lycopene that can have a counteracting deleterious effect on oxidative stress, the molecular delivery using lipid NPs was explored in a human clinical study. The study concluded a protective effect and reduced oxidative damage to proteins in the compromised periodontium tissue.…”

Section: Periodontitis and Peri-implantitis Diseasesmentioning

confidence: 99%

Lipid nanoparticle-based formulations for high-performance dentistry applications

et al. 2023

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Effect of local hIL-10 gene therapy on experimental periodontitis in ovariectomized rats

Abstract: Local hIL-10 gene transfer suppressed alveolar bone resorption in OVX rats, and this effect was probably associated with the decline in the expression of pro-inflammatory cytokines in the periodontal tissues.

Cited by 5 publications

References 37 publications

Diacerein‐induced interleukin‐1β deficiency reduces the inflammatory infiltrate and immunoexpression of matrix metalloproteinase‐8 in periodontitis in rat molars

Diacerein‐induced interleukin‐1β deficiency reduces the inflammatory infiltrate and immunoexpression of matrix metalloproteinase‐8 in periodontitis in rat molars

Genetic Association with Subgingival Bacterial Colonization in Chronic Periodontitis

Lipid nanoparticle-based formulations for high-performance dentistry applications

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