Effect of Local Environment and Protein on the Mechanism of
Action of Superoxide Dismutase

Osman, Roman

doi:10.1159/000469276

Cited by 8 publications

(3 citation statements)

References 30 publications

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“…Arg14' is considered the most important residue for the electrostatic docking of the superoxide ion in the active site channel [33,34]. Additionally, in subunit A, the cyanide N atom hydrogen bonds to three water molecules, W137, W281 and W126 (2.87, 3.18 and 3.28 A, respectively) (see Fig.…”

Section: Resultsmentioning

confidence: 99%

“…A strong electrostatic interaction is observed between the bound cyanide anion and Arg14', the distance between cyanide N and the guanidinium NEH2 atoms being 3.3113.35 A (subunit A/subunit B). Arg14' is considered the most important residue for the electrostatic docking of the superoxide ion in the active site channel [33,34]. Additionally, in subunit A, the cyanide N atom hydrogen bonds to three water molecules, W137, W281 and W126 (2.87, 3.18 and 3.28 A, respectively) (see Fig.…”

Section: Resultsmentioning

confidence: 99%

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Crystal structure of the cyanide‐inhibited Xenopus laevis Cu,Zn superoxide dismutase at 98 K

et al. 1994

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The crystal structure of cyanide-inhibited X. laevis Cu,Zn superoxide dismutase has been studied and refined based on diffraction data collected at 98 K. The final R-factor for the 27,299 reflections in the 10.0-l .7 8, resolution range is 0.170. The cyanide anion, which is a competitive inhibitor expected to mimic the superoxide binding mode, binds directly to the active site copper atom, replacing the coordinated water molecule. Moreover, the anion establishes a strong electrostatic interaction with the guanidinium group of the conserved active site residue Arg14'. The coordination sphere of Cu" is partly altered with respect to the uninhibited enzyme: a displacement of 0.41 8, in subunit A, and 0.27 A in subunit B of the dimeric enzyme is observed for the Cu" ions. Only two ligands in the CL?' coordination sphere (His? and His"*) are significantly affected by cyanide binding, whereas virtually no rearrangement of the Zn" ligands is reported.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Crystal structure of the cyanide‐inhibited Xenopus laevis Cu,Zn superoxide dismutase at 98 K

et al. 1994

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“…SOD analysis Oxygen free radicals are normally neutralized by very efficient systems in the body. These include antioxidant enzymes like SOD (Osman, 1986; Salo et al , 1990). SOD scavenges the superoxide radicals by catalyzing the reactive species into dioxygen and oxygen peroxide, thereby protecting cells against the reactive oxygen species produced by the electric field or other mechanisms (Güler et al , 1996, 2005).…”

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confidence: 99%

Examination of static and 50 Hz electric field effects on tissues by using a hybrid genetic algorithm and neural network

Hardalaç¹,

Güler²

2008

Expert Systems

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The effects of electric fields on tissue are the main subject of many investigations. The importance of this subject comes from the electrical properties of the cell membrane and its sensitivity to changes in electrical conditions. Permeability of membranes to various ions can change by the effect of an electric field depending on their conductivity. The performances of cells and tissues change due to differences between the membrane's permeability to various ions and molecules. The aim of this study was to determine lipid peroxidation and superoxide dismutase (SOD) levels in spleen and testis tissues exposed to different intensities and exposure periods of static and 50 Hz alternating electric fields. The increase in SOD and thiobarbituric acid reactive substance levels of spleen and testis tissues was found to depend significantly on the type of electric field and the exposure period. The experimental results are applied to a hybrid genetic algorithm and neural network as learning data and the training of the feedforward neural network is realized. At the end of this training, without applying electric field to tissues, the determination of the effects of the electric field on tissues by using a computer is predicted by the neural network. After the experiments, the prediction of the hybrid genetic algorithm and neural network approach is on average 99.25%–99.99%.

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Examination of Electric Field Effects on Tissues by Using Back Propagation Neural Network

2005

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The aim of this study is to determine lipid peroxidation and antioxidant enzyme levels in spleen and testis tissues of guinea pigs which were exposed to different intensities and periods of DC (direct current) and AC (alternating current) electric fields. The experimental results are applied to neural networks as learning data and the training of the feed forward neural network is realized. At the end of this training; without applying electric field to the tissues, the determination of the effects of the electric field on tissues by using computer is predicted by the neural network. After the experiments, the prediction of the neural network is averagely 99%.

show abstract

Effect of Local Environment and Protein on the Mechanism of Action of Superoxide Dismutase

Cited by 8 publications

References 30 publications

Crystal structure of the cyanide‐inhibited Xenopus laevis Cu,Zn superoxide dismutase at 98 K

Crystal structure of the cyanide‐inhibited Xenopus laevis Cu,Zn superoxide dismutase at 98 K

Examination of static and 50 Hz electric field effects on tissues by using a hybrid genetic algorithm and neural network

Examination of Electric Field Effects on Tissues by Using Back Propagation Neural Network

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